Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QP552_RS02530 Genome accession   NZ_CP127408
Coordinates   569873..571000 (-) Length   375 a.a.
NCBI ID   WP_004141526.1    Uniprot ID   A0A854E1G1
Organism   Stenotrophomonas sp. 59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 564873..576000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP552_RS02510 - 565289..566278 (+) 990 WP_164137462.1 fumarylacetoacetate hydrolase family protein -
  QP552_RS02515 maiA 566293..566976 (+) 684 WP_286074881.1 maleylacetoacetate isomerase -
  QP552_RS02520 - 567010..568500 (-) 1491 WP_286074882.1 UdgX family uracil-DNA binding protein -
  QP552_RS02525 - 568481..569764 (-) 1284 WP_286074884.1 putative DNA modification/repair radical SAM protein -
  QP552_RS02530 pilU 569873..571000 (-) 1128 WP_004141526.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QP552_RS02535 - 571122..571487 (+) 366 WP_005407961.1 DUF4398 domain-containing protein -
  QP552_RS02540 - 571484..572368 (+) 885 WP_005407962.1 hypothetical protein -
  QP552_RS02545 - 572693..572929 (+) 237 WP_005407964.1 YdcH family protein -
  QP552_RS02550 - 573231..574601 (+) 1371 WP_049397548.1 pyridoxal-phosphate dependent enzyme -
  QP552_RS02555 - 574725..575900 (+) 1176 WP_106550209.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41398.70 Da        Isoelectric Point: 6.3741

>NTDB_id=844139 QP552_RS02530 WP_004141526.1 569873..571000(-) (pilU) [Stenotrophomonas sp. 59]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLLNTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=844139 QP552_RS02530 WP_004141526.1 569873..571000(-) (pilU) [Stenotrophomonas sp. 59]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCACTGGGCAATACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTACCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCCCGATGCCGGTCGT
TTCCGCGTCAATGTGTTCAAGCAGCGCGGTGAAGTCGGCATGGTCATCCGCGCCATCCGCAGCCGGATTCCGAGCATCGA
AGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGTGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCATCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGGAAACAATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGCATCCCTGGAAGAGGGCATGGAGAGTTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCACCATCGAGCAGGAGGAAGCGCTGCGCGCCGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCACGATCCCTACGCGGATTTCTCGGCCGGCGCGCCAAGCATCACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854E1G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

95.733

0.488
  pilU Vibrio cholerae strain A1552

49.435

94.4

0.467
  pilU Acinetobacter baylyi ADP1

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.04

99.467

0.448
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.42

90.133

0.373
  pilT Acinetobacter nosocomialis M2

41.42

90.133

0.373
  pilT Acinetobacter baumannii D1279779

41.42

90.133

0.373
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

41.141

88.8

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.8

0.365
  pilT Neisseria gonorrhoeae MS11

38.905

92.533

0.36
  pilT Neisseria meningitidis 8013

38.905

92.533

0.36