Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QP512_RS02540 Genome accession   NZ_CP127407
Coordinates   568373..569500 (-) Length   375 a.a.
NCBI ID   WP_043398800.1    Uniprot ID   A0AA42X8C5
Organism   Stenotrophomonas sp. 57     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 563373..574500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP512_RS02520 - 563762..564751 (+) 990 WP_286070856.1 fumarylacetoacetate hydrolase family protein -
  QP512_RS02525 maiA 564766..565449 (+) 684 WP_286070857.1 maleylacetoacetate isomerase -
  QP512_RS02530 - 565483..566979 (-) 1497 WP_286070858.1 UdgX family uracil-DNA binding protein -
  QP512_RS02535 - 566960..568243 (-) 1284 WP_286070859.1 putative DNA modification/repair radical SAM protein -
  QP512_RS02540 pilU 568373..569500 (-) 1128 WP_043398800.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QP512_RS02545 - 569622..569987 (+) 366 WP_019660954.1 DUF4398 domain-containing protein -
  QP512_RS02550 - 569984..570868 (+) 885 WP_286070860.1 hypothetical protein -
  QP512_RS02555 - 571193..571429 (+) 237 WP_005407964.1 YdcH family protein -
  QP512_RS02560 - 571730..573100 (+) 1371 WP_286070861.1 pyridoxal-phosphate dependent enzyme -
  QP512_RS02565 - 573224..574408 (+) 1185 WP_286070862.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41384.67 Da        Isoelectric Point: 6.3741

>NTDB_id=844120 QP512_RS02540 WP_043398800.1 568373..569500(-) (pilU) [Stenotrophomonas sp. 57]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=844120 QP512_RS02540 WP_043398800.1 568373..569500(-) (pilU) [Stenotrophomonas sp. 57]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCGCCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTACCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATTGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGTGGCGAGGTCGGCATGGTCATCCGTGCCATCCGCAGCCGCATTCCGAGCATTGA
AGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACGATGGAGGCGG
CCATCGCCTTCGCCGAAACCGGCCACCTTTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCACACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGGGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCCGCCACCGAAGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GTCGTGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGGCTCGCTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCACCATCGAGCAGGAGGAGGCCCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCACGATCCGTACGCGGATTTCTCGGCCGGCGCGCCGAGCATCACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363