Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LDN84_RS21430 Genome accession   NZ_AP024238
Coordinates   4644954..4645997 (-) Length   347 a.a.
NCBI ID   WP_223905769.1    Uniprot ID   -
Organism   Rhodoferax lithotrophicus strain MIZ03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4639954..4650997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDN84_RS21400 (MIZ03_4513) rsmI 4640020..4640961 (-) 942 WP_223905757.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  LDN84_RS21405 (MIZ03_4514) - 4640964..4641356 (+) 393 WP_223905759.1 YraN family protein -
  LDN84_RS21410 (MIZ03_4515) - 4641444..4642043 (+) 600 WP_223905761.1 phosphoheptose isomerase -
  LDN84_RS21415 (MIZ03_4516) - 4642040..4642705 (+) 666 WP_223905763.1 BON domain-containing protein -
  LDN84_RS21420 (MIZ03_4517) - 4642838..4643755 (-) 918 WP_223905765.1 NAD(P)-dependent oxidoreductase -
  LDN84_RS21425 (MIZ03_4518) pilU 4643803..4644939 (-) 1137 WP_223905767.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LDN84_RS21430 (MIZ03_4519) pilT 4644954..4645997 (-) 1044 WP_223905769.1 type IV pilus twitching motility protein PilT Machinery gene
  LDN84_RS21435 (MIZ03_4520) - 4646061..4646753 (+) 693 WP_223905771.1 YggS family pyridoxal phosphate-dependent enzyme -
  LDN84_RS21440 (MIZ03_4521) - 4646768..4647229 (-) 462 WP_223905773.1 Lrp/AsnC ligand binding domain-containing protein -
  LDN84_RS21445 (MIZ03_4522) - 4647233..4649812 (-) 2580 WP_223905775.1 EAL domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38387.12 Da        Isoelectric Point: 6.8807

>NTDB_id=84232 LDN84_RS21430 WP_223905769.1 4644954..4645997(-) (pilT) [Rhodoferax lithotrophicus strain MIZ03]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLDHKQVHAMVYDIMNDSQRKHFEEFLEIDFSFEIDGLAR
FRVNAFNQQRGAGAVFRTIPSKILTLEQLNCPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEFGHILTVED
PIEFVHESKKCLVNQREVGPHTQSFAAALRSALREDPDCILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTRDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=84232 LDN84_RS21430 WP_223905769.1 4644954..4645997(-) (pilT) [Rhodoferax lithotrophicus strain MIZ03]
GTGGATATCACCCAGCTGCTGGCCTTCAGCGTGAAAAACAAAGCATCCGACTTGCACCTCTCCGCCGGGTTGCCGCCGAT
GATCCGGGTGCACGGTGACGTGCGGCGCATCAACGTGGAGCCACTGGACCACAAACAGGTGCATGCCATGGTGTATGACA
TCATGAACGACTCTCAGCGCAAGCATTTTGAAGAGTTTCTGGAGATTGACTTTTCGTTCGAGATTGACGGGCTGGCGCGT
TTCCGGGTGAATGCGTTCAACCAGCAGCGCGGCGCTGGCGCGGTGTTCCGAACGATTCCCAGCAAGATTTTGACGCTGGA
GCAACTCAACTGCCCGAAGATTTTTGGCGAGCTGGCCCTGAAACCGCGTGGTCTGGTGCTGGTGACCGGCCCCACTGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGGTGAACTACCTCAATGAGACCGAGTTTGGCCACATTCTGACGGTGGAAGAC
CCGATTGAATTTGTCCACGAGTCCAAAAAATGCCTGGTTAACCAGCGTGAGGTTGGGCCACACACCCAGAGTTTCGCCGC
CGCTTTGCGCTCAGCCTTGCGCGAAGACCCGGACTGCATTCTGGTCGGTGAAATGCGTGACCTGGAAACCATCCGCCTGG
CCATGACGGCTGCGGAAACCGGTCACCTGGTGTTTGGCACCTTGCACACCAGCAGCGCGGCCAAGACGATTGACCGGATC
ATTGACGTGTTTCCGGCGGAAGAAAAAGAGATGGTGCGCGCCATGCTCTCGGAGTCGCTGCAGGCGGTGATTTCACAAAC
CCTGTGTAAAACCAGAGACGGTCAGGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACCAGTGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCGCAGATGTATTCCAGCATCCAGACCGGCAACAGCGTGGGTATGCAGACGCTGGATCAGAAC
CTGACCGACCTGGTGCGGCGCAACATCATCAGTCCGGCCGAAGCACGCTCCAAAGCCAAAATTCCTGACAACTTCCCGGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.631

97.695

0.729
  pilT Legionella pneumophila strain ERS1305867

74.631

97.695

0.729
  pilT Neisseria meningitidis 8013

68.235

97.983

0.669
  pilT Neisseria gonorrhoeae MS11

67.941

97.983

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.542

0.412
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412

Multiple sequence alignment