Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QPR70_RS18675 Genome accession   NZ_CP126761
Coordinates   3874723..3875691 (-) Length   322 a.a.
NCBI ID   WP_112018250.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain Ehh_24     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3869723..3880691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPR70_RS18645 (QPR70_18620) - 3870588..3871085 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  QPR70_RS18650 (QPR70_18625) endA 3871180..3871887 (+) 708 WP_003860034.1 deoxyribonuclease I -
  QPR70_RS18655 (QPR70_18630) rsmE 3871939..3872670 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QPR70_RS18660 (QPR70_18635) gshB 3872702..3873649 (+) 948 WP_003860032.1 glutathione synthase -
  QPR70_RS18665 (QPR70_18640) - 3873724..3874284 (+) 561 WP_003860031.1 YqgE/AlgH family protein -
  QPR70_RS18670 (QPR70_18645) ruvX 3874284..3874700 (+) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  QPR70_RS18675 (QPR70_18650) pilT 3874723..3875691 (-) 969 WP_112018250.1 type IV pilus twitching motility protein PilT Machinery gene
  QPR70_RS18680 (QPR70_18655) - 3875709..3876410 (+) 702 WP_032610102.1 YggS family pyridoxal phosphate-dependent enzyme -
  QPR70_RS18685 (QPR70_18660) - 3876432..3876998 (+) 567 WP_003860024.1 YggT family protein -
  QPR70_RS18690 (QPR70_18665) yggU 3876995..3877291 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  QPR70_RS18695 (QPR70_18670) - 3877295..3877888 (+) 594 WP_022651831.1 XTP/dITP diphosphatase -
  QPR70_RS18700 (QPR70_18675) hemW 3877881..3879029 (+) 1149 WP_045332868.1 radical SAM family heme chaperone HemW -
  QPR70_RS18705 (QPR70_18680) - 3879086..3879448 (+) 363 WP_023295414.1 endonuclease domain-containing protein -
  QPR70_RS18710 (QPR70_18685) - 3879561..3880277 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  QPR70_RS18715 (QPR70_18690) - 3880334..3880660 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 35295.22 Da        Isoelectric Point: 6.0405

>NTDB_id=839797 QPR70_RS18675 WP_112018250.1 3874723..3875691(-) (pilT) [Enterobacter hormaechei strain Ehh_24]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RA

Nucleotide


Download         Length: 969 bp        

>NTDB_id=839797 QPR70_RS18675 WP_112018250.1 3874723..3875691(-) (pilT) [Enterobacter hormaechei strain Ehh_24]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCCTGGTGGGCAAATGGGCAGGTGGATTTTGCCGTTACCCTCGCAGACCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.097

99.068

0.506
  pilT Vibrio cholerae strain A1552

51.097

99.068

0.506
  pilT Legionella pneumophila strain Lp02

48.758

100

0.488
  pilT Legionella pneumophila strain ERS1305867

48.758

100

0.488
  pilT Pseudomonas stutzeri DSM 10701

48.125

99.379

0.478
  pilT Neisseria meningitidis 8013

47.664

99.689

0.475
  pilT Acinetobacter baumannii D1279779

47.5

99.379

0.472
  pilT Acinetobacter nosocomialis M2

47.5

99.379

0.472
  pilT Acinetobacter baumannii strain A118

47.5

99.379

0.472
  pilT Neisseria gonorrhoeae MS11

47.352

99.689

0.472
  pilT Pseudomonas aeruginosa PAK

47.188

99.379

0.469
  pilT Acinetobacter baylyi ADP1

46.894

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

100

0.438
  pilU Vibrio cholerae strain A1552

38.509

100

0.385
  pilU Pseudomonas stutzeri DSM 10701

38.318

99.689

0.382
  pilB Haemophilus influenzae 86-028NP

32.955

100

0.36