Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QPX65_RS13150 Genome accession   NZ_CP126698
Coordinates   2758775..2759881 (-) Length   368 a.a.
NCBI ID   WP_011079463.1    Uniprot ID   A0A3Q0L3V4
Organism   Vibrio vulnificus strain VV2018     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2753775..2764881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPX65_RS13125 (QPX65_13125) - 2754350..2755684 (-) 1335 WP_065090836.1 hypothetical protein -
  QPX65_RS13130 (QPX65_13130) rsmE 2755921..2756652 (+) 732 WP_011079467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QPX65_RS13135 (QPX65_13135) gshB 2756667..2757620 (+) 954 WP_285454591.1 glutathione synthase -
  QPX65_RS13140 (QPX65_13140) - 2757675..2758238 (+) 564 WP_011079465.1 YqgE/AlgH family protein -
  QPX65_RS13145 (QPX65_13145) ruvX 2758260..2758682 (+) 423 WP_011079464.1 Holliday junction resolvase RuvX -
  QPX65_RS13150 (QPX65_13150) pilU 2758775..2759881 (-) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QPX65_RS13155 (QPX65_13155) pilT 2759900..2760937 (-) 1038 WP_011079462.1 type IV pilus twitching motility protein PilT Machinery gene
  QPX65_RS13160 (QPX65_13160) - 2760962..2761672 (+) 711 WP_011079461.1 YggS family pyridoxal phosphate-dependent enzyme -
  QPX65_RS13165 (QPX65_13165) proC 2761707..2762525 (+) 819 WP_285454596.1 pyrroline-5-carboxylate reductase -
  QPX65_RS13170 (QPX65_13170) - 2762567..2763124 (+) 558 WP_013571095.1 YggT family protein -
  QPX65_RS13175 (QPX65_13175) yggU 2763124..2763414 (+) 291 WP_060530713.1 DUF167 family protein YggU -
  QPX65_RS13180 (QPX65_13180) - 2763457..2763888 (+) 432 WP_285454601.1 DUF4426 domain-containing protein -
  QPX65_RS13185 (QPX65_13185) - 2763899..2764501 (+) 603 WP_072603529.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41245.35 Da        Isoelectric Point: 6.7296

>NTDB_id=839656 QPX65_RS13150 WP_011079463.1 2758775..2759881(-) (pilU) [Vibrio vulnificus strain VV2018]
MDLNKILEGMITLKASDLYITVGAPVLFRVDGELRPQGEKLSQIEVAQLLDGAMDEERRADFRKSRESNFAIVRDSGRFR
VSAFYQRELPGAVIRRIETNIPTFEELKLPHVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEVLLNSPRVSDLIRRGDLHELKMTMAKSKEVGMQTFDQSLY
TLVVEGKISEEDAMHSADSANDLRLMLKTQRGDISSPGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=839656 QPX65_RS13150 WP_011079463.1 2758775..2759881(-) (pilU) [Vibrio vulnificus strain VV2018]
ATGGATTTAAACAAGATTTTAGAAGGGATGATCACGCTTAAAGCGTCGGATCTTTATATCACGGTTGGTGCCCCCGTGCT
GTTTCGTGTCGATGGCGAGCTGCGCCCGCAAGGCGAAAAATTATCGCAAATTGAAGTCGCTCAACTGCTGGATGGGGCGA
TGGACGAAGAGCGGCGCGCAGACTTTCGCAAAAGCCGCGAGTCAAACTTTGCCATTGTGCGAGATTCGGGGCGTTTTCGT
GTCAGTGCCTTCTACCAACGAGAGCTTCCTGGTGCGGTGATTCGCCGCATTGAAACCAACATTCCAACCTTTGAGGAACT
TAAGCTGCCTCATGTTTTGCAAGACTTAGCGATTGCCAAACGTGGATTGGTGCTGGTGGTGGGCGCGACGGGCTCTGGTA
AATCGACCACCATGGCTGCGATGACAGGATATCGAAATACCCACCGTAGTGGACACATTTTGACGGTGGAAGATCCGATT
GAATTTGTCCACGAACATAAACGCTGTATTGTCACCCAGCGTGAAGTCGGTTTGGATACGGAAAGCTATGAAGTCGCACT
AAAGAACTCGCTGCGTCAAGCGCCAGATATGATTCTAATTGGTGAGATTCGCAGTCGGGAAACCATGGAATACGCCATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCGACACTGCATGCCAATAACGCCAACCAAGCACTGGAGCGCATCTTACAC
TTGGTGCCTAAAGAACAAAAAGAGCAGTTTTTGTTTGACCTTTCCATGAACTTAAAAGGGGTAGTTGGTCAGCAGTTGAT
TCGTGATAAAAATGGTAAAGGTCGTCACGGTGTGTTCGAGGTGTTACTTAACAGCCCGCGCGTGTCGGATTTGATCCGTC
GTGGCGATTTGCATGAGCTGAAAATGACTATGGCGAAGTCGAAAGAAGTCGGTATGCAGACCTTTGACCAATCACTCTAT
ACATTAGTAGTGGAAGGCAAAATCAGCGAAGAAGACGCCATGCACAGTGCCGATTCGGCCAACGATTTACGTTTGATGCT
GAAGACGCAGCGAGGTGATATTAGTTCCCCCGGCTCATTGGCGAACGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3V4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

100

0.842
  pilU Pseudomonas stutzeri DSM 10701

59.429

95.109

0.565
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.753

88.043

0.394
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.761

0.361
  pilT Vibrio cholerae strain A1552

39.82

90.761

0.361