Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F0321_RS17885 Genome accession   NZ_CP126610
Coordinates   3768988..3769968 (-) Length   326 a.a.
NCBI ID   WP_126329735.1    Uniprot ID   -
Organism   Enterobacter asburiae strain E2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3763988..3774968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0321_RS17855 (F0321_17865) - 3764864..3765361 (+) 498 WP_126329729.1 SprT family zinc-dependent metalloprotease -
  F0321_RS17860 (F0321_17870) endA 3765456..3766163 (+) 708 WP_126329731.1 deoxyribonuclease I -
  F0321_RS17865 (F0321_17875) rsmE 3766215..3766946 (+) 732 WP_024906558.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  F0321_RS17870 (F0321_17880) gshB 3766966..3767913 (+) 948 WP_029739222.1 glutathione synthase -
  F0321_RS17875 (F0321_17885) - 3768001..3768561 (+) 561 WP_126329733.1 YqgE/AlgH family protein -
  F0321_RS17880 (F0321_17890) ruvX 3768561..3768977 (+) 417 WP_023326041.1 Holliday junction resolvase RuvX -
  F0321_RS17885 (F0321_17895) pilT 3768988..3769968 (-) 981 WP_126329735.1 type IV pilus twitching motility protein PilT Machinery gene
  F0321_RS17890 (F0321_17900) - 3769986..3770687 (+) 702 WP_126329737.1 YggS family pyridoxal phosphate-dependent enzyme -
  F0321_RS17895 (F0321_17905) - 3770709..3771275 (+) 567 WP_023309130.1 YggT family protein -
  F0321_RS17900 (F0321_17910) yggU 3771272..3771568 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  F0321_RS17905 (F0321_17915) - 3771572..3772165 (+) 594 WP_048979374.1 XTP/dITP diphosphatase -
  F0321_RS17910 (F0321_17920) hemW 3772158..3773306 (+) 1149 WP_126329739.1 radical SAM family heme chaperone HemW -
  F0321_RS17915 (F0321_17925) - 3773342..3773506 (+) 165 Protein_3500 DUF559 domain-containing protein -
  F0321_RS17920 (F0321_17930) - 3773517..3774668 (-) 1152 Protein_3501 IS481 family transposase -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35519.56 Da        Isoelectric Point: 6.9108

>NTDB_id=839138 F0321_RS17885 WP_126329735.1 3768988..3769968(-) (pilT) [Enterobacter asburiae strain E2]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVAGGQRLRG
SAFKQMRGVSLTLRLLPRSCPQLSSLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=839138 F0321_RS17885 WP_126329735.1 3768988..3769968(-) (pilT) [Enterobacter asburiae strain E2]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGTAGATTAGGCCGGCTTGAGCCTGCGCCCTTTCCGCCTCCCGATGTCGGAGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGACAGGTGGATTTTGCCGCGACGGTGGCGGGAGGCCAGCGCCTGCGCGGG
AGTGCCTTTAAGCAGATGCGCGGAGTTTCTTTGACGCTGCGGCTGTTGCCACGTAGCTGCCCGCAGCTCTCTTCGCTGGG
CGCGCCGCGGGTAATCCCGGAACTGTTGTCAAATGACGCCGGGCTGATTCTGGTCACCGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTAAACCACCATGCTGATGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCTTGATCCAGCAGCGGGAGATAGGTCAGCACAGCCCGTCATTTGCCGAAGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGTTAACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGAGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAACTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGTTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain Lp02

49.689

98.773

0.491
  pilT Legionella pneumophila strain ERS1305867

49.689

98.773

0.491
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362