Detailed information NTG0837535

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: U8327_RS06340
• Genome accession: NZ_CP141765
• Coordinates: 1308088..1309068 (-)
• Length: 326 a.a.
• NCBI ID: WP_071986503.1
• Uniprot ID: -
• Organism: Escherichia coli strain HYM1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1303088..1314068

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U8327_RS06310 (U8327_06310)	yggI	1303812..1304309 (+)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-
U8327_RS06315 (U8327_06315)	endA	1304404..1305111 (+)	708	WP_000286497.1	deoxyribonuclease I	-
U8327_RS06320 (U8327_06320)	rsmE	1305191..1305922 (+)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
U8327_RS06325 (U8327_06325)	gshB	1305935..1306885 (+)	951	WP_000593273.1	glutathione synthase	-
U8327_RS06330 (U8327_06330)	yqgE	1306994..1307557 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
U8327_RS06335 (U8327_06335)	ruvX	1307557..1307973 (+)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
U8327_RS06340 (U8327_06340)	pilT	1308088..1309068 (-)	981	WP_071986503.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
U8327_RS06345 (U8327_06345)	yggS	1309086..1309790 (+)	705	WP_000997806.1	pyridoxal phosphate homeostasis protein	-
U8327_RS06350 (U8327_06350)	yggT	1309808..1310374 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
U8327_RS06355 (U8327_06355)	yggU	1310371..1310661 (+)	291	WP_001277220.1	DUF167 family protein YggU	-
U8327_RS06360 (U8327_06360)	rdgB	1310669..1311262 (+)	594	WP_001174754.1	XTP/dITP diphosphatase	-
U8327_RS06365 (U8327_06365)	hemW	1311255..1312391 (+)	1137	WP_000239962.1	radical SAM family heme chaperone HemW	-
U8327_RS06370 (U8327_06370)	-	1312704..1313690 (+)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35968.21 Da
• Isoelectric Point: 5.9975

>NTDB_id=837535 U8327_RS06340 WP_071986503.1 1308088..1309068(-) (pilT) [Escherichia coli strain HYM1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGVILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASRRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINIPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=837535 U8327_RS06340 WP_071986503.1 1308088..1309068(-) (pilT) [Escherichia coli strain HYM1]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCGTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCCAGCCGGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACATTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTAGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	48.93	100	0.491
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.93	100	0.491
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter nosocomialis M2	46.789	100	0.469
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.789	100	0.469
pilT	Acinetobacter baumannii strain A118	46.789	100	0.469
pilT	Pseudomonas stutzeri DSM 10701	45.872	100	0.46
pilT	Pseudomonas aeruginosa PAK	45.566	100	0.457
pilT	Legionella pneumophila strain Lp02	44.648	100	0.448
pilT	Legionella pneumophila strain ERS1305867	44.648	100	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	39.21	100	0.396
pilU	Pseudomonas stutzeri DSM 10701	36.667	100	0.371
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362