Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VA249_RS12180 Genome accession   NZ_AP024165
Coordinates   2656013..2657053 (-) Length   346 a.a.
NCBI ID   WP_128810138.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain 04Ya108     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2651013..2662053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VA249_RS12150 (VYA_23050) endA 2651107..2651802 (+) 696 WP_221068141.1 deoxyribonuclease I -
  VA249_RS12155 (VYA_23060) rsmE 2651938..2652669 (+) 732 WP_221069946.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VA249_RS12160 (VYA_23070) gshB 2652684..2653634 (+) 951 WP_221068142.1 glutathione synthase -
  VA249_RS12165 (VYA_23080) - 2653774..2654337 (+) 564 WP_221068143.1 YqgE/AlgH family protein -
  VA249_RS12170 (VYA_23090) ruvX 2654368..2654793 (+) 426 WP_221068144.1 Holliday junction resolvase RuvX -
  VA249_RS12175 (VYA_23100) pilU 2654879..2655985 (-) 1107 WP_221068145.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VA249_RS12180 (VYA_23110) pilT 2656013..2657053 (-) 1041 WP_128810138.1 type IV pilus twitching motility protein PilT Machinery gene
  VA249_RS12185 (VYA_23120) - 2657082..2657792 (+) 711 WP_221068146.1 YggS family pyridoxal phosphate-dependent enzyme -
  VA249_RS12190 (VYA_23130) proC 2657967..2658785 (+) 819 WP_221068147.1 pyrroline-5-carboxylate reductase -
  VA249_RS12195 (VYA_23140) - 2658838..2659395 (+) 558 WP_221068148.1 YggT family protein -
  VA249_RS12200 (VYA_23150) yggU 2659395..2659685 (+) 291 WP_221068149.1 DUF167 family protein YggU -
  VA249_RS12205 (VYA_23160) - 2659749..2660180 (+) 432 WP_211915945.1 DUF4426 domain-containing protein -
  VA249_RS12210 (VYA_23170) - 2660275..2660877 (+) 603 WP_221068150.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38504.97 Da        Isoelectric Point: 6.5577

>NTDB_id=83738 VA249_RS12180 WP_128810138.1 2656013..2657053(-) (pilT) [Vibrio alfacsensis strain 04Ya108]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFEMHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLDELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSHDEAEKKVGLEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=83738 VA249_RS12180 WP_128810138.1 2656013..2657053(-) (pilT) [Vibrio alfacsensis strain 04Ya108]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCATCAGATCTACATCTTTCTGCCGGTGTTCCCCCAAT
GGTACGTATTGATGGGGACGTAAGAAAGCTTGGCATACCAGCGTTTACACACCAAGAAGTGCATCGTCTCGTTTTTGAAA
TCATGAACGATGCACAACGCAGTGAATTCGAAGAAAAACTCGAAGTCGATTTTTCCTTTGAAATGCATAATGTCGGTCGT
TTCCGTGTGAACGCTTTCAATCAATCTCGAGGTTGTGCTGCTGTCTTTCGTACCATCCCAAGTAGCATTCCAACCTTGGA
TGAGTTAGAAGCGCCAGAAATTTTCAAAAAAATAGCGAATGCTGAAAAAGGTTTGGTGTTGGTGACAGGGCCAACGGGTT
CGGGTAAATCGACAACACTCGCTGCTATTGTTGACTACATTAACCGCAATCACAACAAGCATATCTTAACAATTGAAGAT
CCGATCGAGTTTGTTCATACCAATAACAAATGTTTGATCAACCAACGTGAAGTTCACCGTGATACCCACAGTTTCCAAAA
TGCACTGCGTAGCGCGCTCCGTGAAGACCCGGACGTGATTTTGGTCGGGGAAATGCGTGATAAGGAAACCATCAGCCTAG
CACTTACAGCCGCAGAAACCGGCCACCTTGTTTTTGGTACGCTGCACACAAGTTCAGCGGCGAAAACCATCGACCGTATT
ATTGACGTATTCCCAGGCAGCGATAAAGACATGGTACGCTCAATGCTGTCTGAATCACTGCGCTCTGTTATTGCCCAAAA
GCTATTAAAGCGAAATGGTGGCGGCCGGATTGCTTGTCATGAAATCATGATGGCAACCCCTGCTATTCGTAACCTTATTC
GCGAAGATAAAGTTGCTCAGATGTACTCTATTATTCAAACGGGTGCCGCTCATGGAATGCAAACCATGGAACAAAATGCG
CGTCAGTTGATGGCTCAAGGTATGGTTTCTCATGATGAGGCCGAAAAAAAGGTTGGCCTTGAAGCACAACAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

85.797

99.711

0.855
  pilT Vibrio cholerae strain A1552

85.797

99.711

0.855
  pilT Acinetobacter baumannii D1279779

71.856

96.532

0.694
  pilT Acinetobacter baumannii strain A118

71.856

96.532

0.694
  pilT Acinetobacter nosocomialis M2

71.557

96.532

0.691
  pilT Acinetobacter baylyi ADP1

71.212

95.376

0.679
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.399

0.676
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Legionella pneumophila strain Lp02

67.665

96.532

0.653
  pilT Legionella pneumophila strain ERS1305867

67.665

96.532

0.653
  pilT Neisseria meningitidis 8013

64.201

97.688

0.627
  pilT Neisseria gonorrhoeae MS11

63.905

97.688

0.624
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.28

100

0.494
  pilU Vibrio cholerae strain A1552

41.791

96.821

0.405
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.821

0.393
  pilU Acinetobacter baylyi ADP1

38.757

97.688

0.379

Multiple sequence alignment