Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QNN06_RS24625 Genome accession   NZ_CP126144
Coordinates   5007770..5008819 (-) Length   349 a.a.
NCBI ID   WP_284036111.1    Uniprot ID   -
Organism   Neobacillus sp. 114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5002770..5013819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNN06_RS24600 - 5003392..5004177 (-) 786 WP_284036107.1 PilN domain-containing protein -
  QNN06_RS24605 pilM 5004177..5005172 (-) 996 WP_284036108.1 pilus assembly protein PilM -
  QNN06_RS24610 - 5005189..5005965 (-) 777 WP_349654922.1 prepilin peptidase -
  QNN06_RS24615 - 5005952..5006425 (-) 474 WP_284036109.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QNN06_RS24620 - 5006560..5007777 (-) 1218 WP_284036110.1 type II secretion system F family protein -
  QNN06_RS24625 pilT 5007770..5008819 (-) 1050 WP_284036111.1 type IV pilus twitching motility protein PilT Machinery gene
  QNN06_RS24630 - 5008832..5010493 (-) 1662 WP_284036112.1 ATPase, T2SS/T4P/T4SS family -
  QNN06_RS24635 - 5010508..5011824 (-) 1317 WP_284036113.1 G5 domain-containing protein -
  QNN06_RS24640 - 5011876..5012703 (-) 828 WP_284036114.1 hypothetical protein -
  QNN06_RS24645 - 5012866..5013282 (+) 417 WP_284036115.1 type II secretion system protein -
  QNN06_RS24650 - 5013292..5013756 (+) 465 WP_284036116.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38661.68 Da        Isoelectric Point: 9.5413

>NTDB_id=836963 QNN06_RS24625 WP_284036111.1 5007770..5008819(-) (pilT) [Neobacillus sp. 114]
MKEKIEAILRAAIEYKASDIHLTVGVPPVFRINGEIKRYGTEKLMPVDTEAMAKAIIPEDKWDTFKEKGELDFSYGAPGI
SRFRVNSYHQRKSVSLALRAVASKTPTIEELDLPGIITKLVQKPQGLILVTGPTGSGKSTTLASMIDYMNRTMRKHIITL
EDPIEYLHRHGNSIIDQREVGYDTMNYASGLKAALRQDPDVILVGEMRDLETIGIAITAAETGHLVFGTLHTSSAPTTIN
RIIDVFPPAQQPQIRVQLASVLVGIISQRLFPTADKKGRRAATEILVNNPAIANLIRSEKIHQIQSTMQTSRAQGMHTLE
MSIRDLIGRGVIQQEAAAQYLQEKMMQNG

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=836963 QNN06_RS24625 WP_284036111.1 5007770..5008819(-) (pilT) [Neobacillus sp. 114]
ATGAAAGAAAAGATTGAGGCAATACTGCGTGCAGCGATTGAATATAAAGCTTCAGACATTCACTTAACTGTAGGTGTTCC
CCCTGTATTCAGAATTAATGGCGAAATTAAAAGGTACGGAACGGAAAAGTTAATGCCCGTTGATACCGAGGCAATGGCGA
AAGCGATTATTCCCGAGGATAAATGGGATACATTCAAGGAAAAGGGGGAGCTTGACTTTTCCTATGGTGCTCCTGGGATC
TCACGCTTCCGTGTCAATTCATATCATCAACGTAAAAGTGTGTCTTTAGCCCTTAGGGCAGTCGCTTCCAAAACACCTAC
AATAGAGGAATTGGATTTACCCGGCATCATTACTAAGTTAGTCCAAAAACCTCAAGGCCTTATTTTAGTAACAGGTCCGA
CAGGAAGCGGAAAATCTACCACGCTTGCTTCCATGATTGATTATATGAATAGAACGATGCGTAAACATATCATTACGCTT
GAAGACCCGATTGAATATCTTCATCGGCATGGAAATTCCATTATCGATCAGCGTGAAGTTGGGTATGATACGATGAACTA
TGCCAGCGGGTTAAAAGCAGCCCTTCGACAGGACCCTGATGTTATTTTGGTAGGGGAGATGCGCGACTTAGAAACCATTG
GTATTGCCATTACTGCGGCAGAAACGGGGCACTTGGTCTTTGGGACACTTCATACCTCAAGTGCACCAACAACGATTAAC
CGGATTATTGACGTATTTCCACCAGCCCAGCAGCCGCAAATTAGAGTTCAGTTGGCTTCTGTTCTCGTGGGAATCATATC
ACAGCGTTTGTTTCCAACCGCGGATAAGAAAGGACGTAGGGCTGCCACTGAAATCTTGGTAAACAATCCGGCTATTGCGA
ATTTAATTCGGAGTGAGAAAATCCATCAAATTCAAAGCACAATGCAAACGTCCCGTGCCCAGGGAATGCACACCCTTGAA
ATGAGTATCCGTGACTTGATTGGCCGTGGAGTCATACAGCAAGAGGCAGCAGCACAATACTTGCAGGAAAAGATGATGCA
AAATGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.439

93.983

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.507

97.708

0.464
  pilT Vibrio cholerae strain A1552

47.507

97.708

0.464
  pilT Acinetobacter baumannii strain A118

46.847

95.415

0.447
  pilT Acinetobacter baumannii D1279779

46.847

95.415

0.447
  pilT Acinetobacter nosocomialis M2

46.847

95.415

0.447
  pilT Neisseria gonorrhoeae MS11

45.723

97.135

0.444
  pilT Neisseria meningitidis 8013

45.428

97.135

0.441
  pilT Pseudomonas aeruginosa PAK

45.97

95.989

0.441
  pilT Acinetobacter baylyi ADP1

46.505

94.269

0.438
  pilT Pseudomonas stutzeri DSM 10701

45.075

95.989

0.433
  pilT Legionella pneumophila strain Lp02

43.582

95.989

0.418
  pilT Legionella pneumophila strain ERS1305867

43.582

95.989

0.418
  pilU Pseudomonas stutzeri DSM 10701

42.216

95.702

0.404
  pilU Acinetobacter baylyi ADP1

41.25

91.691

0.378
  pilU Vibrio cholerae strain A1552

40.123

92.837

0.372