Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QNH48_RS23435 Genome accession   NZ_CP126115
Coordinates   4735836..4736885 (-) Length   349 a.a.
NCBI ID   WP_283955869.1    Uniprot ID   A0AA95N7M8
Organism   Neobacillus sp. YX16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4730836..4741885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH48_RS23410 (QNH48_23410) - 4731383..4732198 (-) 816 WP_283952206.1 PilN domain-containing protein -
  QNH48_RS23415 (QNH48_23415) pilM 4732195..4733190 (-) 996 WP_283952207.1 pilus assembly protein PilM -
  QNH48_RS23420 (QNH48_23420) - 4733206..4733967 (-) 762 WP_283952208.1 A24 family peptidase -
  QNH48_RS23425 (QNH48_23425) - 4733968..4734477 (-) 510 WP_283955868.1 type II secretion system protein -
  QNH48_RS23430 (QNH48_23430) - 4734638..4735843 (-) 1206 WP_283952209.1 type II secretion system F family protein -
  QNH48_RS23435 (QNH48_23435) pilT 4735836..4736885 (-) 1050 WP_283955869.1 type IV pilus twitching motility protein PilT Machinery gene
  QNH48_RS23440 (QNH48_23440) - 4736896..4738551 (-) 1656 WP_283952210.1 ATPase, T2SS/T4P/T4SS family -
  QNH48_RS23445 (QNH48_23445) - 4738576..4739862 (-) 1287 WP_283952211.1 G5 domain-containing protein -
  QNH48_RS23450 (QNH48_23450) - 4739881..4740597 (-) 717 WP_283952212.1 hypothetical protein -
  QNH48_RS23455 (QNH48_23455) - 4740829..4741257 (+) 429 WP_283952213.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QNH48_RS23460 (QNH48_23460) - 4741269..4741706 (+) 438 WP_283952214.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38760.77 Da        Isoelectric Point: 8.4278

>NTDB_id=836656 QNH48_RS23435 WP_283955869.1 4735836..4736885(-) (pilT) [Neobacillus sp. YX16]
MKERIDNILRAAVEFKASDIHITVGVPPVVRINGELKRYGQEKLMPADTEEMARAIIPENMWDLFVEKGELDFSYGVHGS
SRFRVNAYHQRKSVAMALRVVSSTVPSIQDLDLPAIVPKLVEKPQGLILVTGPTGSGKSTTLASMIDYMNRTMRKHIVTL
EDPIEYLHRHGNSIIDQREVGFDTQSYANGLKSALRQDPDVILVGEMRDLETMGIAITAAETGHLVLGTLHTSSAPSTIN
RIIDVFPPAQQPQIRVQLASVLVGVISQRLFPTVDKKGRKAATEIMVNNPAIANLIRNEKIHQIQSTMQTSRAQGMHTLE
MSIRDLLDRNLIQKEAAAKYLQEKAMLDG

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=836656 QNH48_RS23435 WP_283955869.1 4735836..4736885(-) (pilT) [Neobacillus sp. YX16]
GTGAAAGAAAGAATTGATAATATTTTACGTGCAGCAGTTGAATTCAAAGCATCAGATATTCATATTACGGTTGGTGTTCC
TCCAGTCGTTCGTATAAACGGAGAATTAAAACGCTATGGCCAGGAAAAACTAATGCCCGCTGACACAGAAGAAATGGCAA
GAGCGATAATTCCTGAGAACATGTGGGATCTTTTTGTTGAAAAAGGTGAACTTGATTTCTCCTATGGTGTCCATGGTAGT
TCTCGATTTCGTGTAAATGCCTATCATCAACGTAAAAGTGTCGCCATGGCATTGAGGGTGGTTTCCTCAACCGTACCATC
GATTCAGGATTTGGATTTACCGGCCATTGTCCCAAAGCTAGTTGAAAAGCCACAAGGTCTTATTTTAGTTACGGGTCCGA
CAGGAAGTGGTAAATCTACTACACTTGCTTCAATGATTGATTACATGAACCGAACAATGAGGAAACATATTGTTACTCTT
GAAGACCCAATAGAATATCTCCATCGCCATGGAAACTCTATTATTGACCAACGTGAAGTTGGGTTTGATACACAGTCCTA
TGCAAATGGTTTAAAGTCAGCTCTTAGACAGGACCCGGACGTGATTCTTGTCGGAGAAATGCGTGATTTAGAAACAATGG
GGATTGCGATAACGGCTGCAGAAACGGGTCACTTGGTACTAGGAACACTCCACACTTCAAGTGCACCTTCAACCATCAAC
AGGATCATTGATGTATTTCCGCCAGCGCAACAGCCGCAAATACGTGTTCAACTCGCTTCTGTATTGGTTGGTGTTATATC
TCAGCGTTTATTTCCAACCGTTGATAAAAAAGGCAGAAAAGCGGCAACTGAAATTATGGTTAATAATCCGGCCATAGCCA
ATCTCATTCGAAATGAAAAGATTCATCAAATTCAAAGTACCATGCAAACCTCCCGTGCCCAAGGAATGCATACACTTGAA
ATGAGTATAAGAGACCTGCTGGATAGAAACCTAATCCAAAAAGAAGCAGCAGCGAAATATTTACAAGAGAAGGCGATGCT
GGATGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.134

93.983

0.49
  pilT Vibrio cholerae strain A1552

47.093

98.567

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.093

98.567

0.464
  pilT Pseudomonas stutzeri DSM 10701

46.377

98.854

0.458
  pilT Neisseria gonorrhoeae MS11

46.608

97.135

0.453
  pilT Legionella pneumophila strain Lp02

45.773

98.281

0.45
  pilT Legionella pneumophila strain ERS1305867

45.773

98.281

0.45
  pilT Neisseria meningitidis 8013

46.313

97.135

0.45
  pilT Acinetobacter nosocomialis M2

46.547

95.415

0.444
  pilT Acinetobacter baumannii D1279779

46.547

95.415

0.444
  pilT Acinetobacter baumannii strain A118

46.547

95.415

0.444
  pilT Pseudomonas aeruginosa PAK

45.161

97.708

0.441
  pilT Acinetobacter baylyi ADP1

46.201

94.269

0.436
  pilU Pseudomonas stutzeri DSM 10701

41.742

95.415

0.398
  pilU Acinetobacter baylyi ADP1

40.95

96.562

0.395
  pilU Vibrio cholerae strain A1552

40.541

95.415

0.387