Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QNH20_RS19830 Genome accession   NZ_CP126110
Coordinates   4045228..4046277 (-) Length   349 a.a.
NCBI ID   WP_283919691.1    Uniprot ID   -
Organism   Neobacillus sp. WH10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4040228..4051277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH20_RS19805 (QNH20_19805) - 4040856..4041701 (-) 846 WP_283919686.1 PilN domain-containing protein -
  QNH20_RS19810 (QNH20_19810) pilM 4041701..4042690 (-) 990 WP_283919687.1 pilus assembly protein PilM -
  QNH20_RS19815 (QNH20_19815) - 4042706..4043446 (-) 741 WP_283919688.1 A24 family peptidase -
  QNH20_RS19820 (QNH20_19820) - 4043458..4043907 (-) 450 WP_283919689.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QNH20_RS19825 (QNH20_19825) - 4044030..4045235 (-) 1206 WP_283919690.1 type II secretion system F family protein -
  QNH20_RS19830 (QNH20_19830) pilT 4045228..4046277 (-) 1050 WP_283919691.1 type IV pilus twitching motility protein PilT Machinery gene
  QNH20_RS19835 (QNH20_19835) - 4046290..4047951 (-) 1662 WP_283923456.1 ATPase, T2SS/T4P/T4SS family -
  QNH20_RS19840 (QNH20_19840) - 4047968..4049278 (-) 1311 WP_283919692.1 G5 domain-containing protein -
  QNH20_RS19845 (QNH20_19845) - 4049349..4050149 (-) 801 WP_283919693.1 hypothetical protein -
  QNH20_RS19850 (QNH20_19850) - 4050364..4050819 (+) 456 WP_283919694.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38878.97 Da        Isoelectric Point: 8.4502

>NTDB_id=836453 QNH20_RS19830 WP_283919691.1 4045228..4046277(-) (pilT) [Neobacillus sp. WH10]
MKEKIENILRAALEFKASDIHITVGAPPVFRIHGEIKRYGKDVLQPVDTEEIAKLIVPENMWDLFKEKGELDFSYALQGV
ARFRVNAFHQRKSISLALRVVPSKTPTLEELDLPEIISKLVEKPQGLILVTGPTGSGKSTTLASMIDYMNKTMRKHIITL
EDPIEYLHKHGNSIIDQREVGFDTKSYANGLKGALRQDPDVILVGEMRDLETMSIAITAAETGHLVLGTLHTSSAPTTIN
RIIDVFPSGQQPQIRTQLASVLVGVISQRLFQTADRKGRKAATEILVNNPAIANLIRNEKIHQIQSTMQTSRAQGMHTLE
MSIKDLLDRGLIHNEAAAQYLHEKMMLNG

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=836453 QNH20_RS19830 WP_283919691.1 4045228..4046277(-) (pilT) [Neobacillus sp. WH10]
ATGAAGGAAAAAATCGAAAATATACTGAGAGCAGCGCTTGAATTTAAAGCTTCTGATATTCATATAACAGTAGGAGCACC
GCCTGTATTTCGGATACATGGTGAAATAAAACGATATGGTAAAGATGTATTGCAGCCTGTAGACACAGAGGAAATTGCTA
AACTAATCGTACCTGAAAACATGTGGGACTTATTTAAAGAGAAAGGTGAGCTCGATTTCTCTTATGCCCTTCAAGGTGTA
GCGCGCTTCCGTGTCAATGCATTCCATCAACGGAAAAGTATCTCCCTGGCCTTAAGGGTAGTTCCGTCTAAAACTCCGAC
TTTGGAAGAGTTAGATTTACCAGAAATCATCTCCAAACTAGTGGAGAAACCACAGGGACTGATCCTTGTTACAGGACCAA
CGGGAAGCGGTAAGTCAACAACTCTTGCATCAATGATTGATTATATGAACAAAACGATGCGCAAGCATATCATTACACTT
GAAGATCCAATTGAATATCTACATAAACATGGAAATTCAATAATCGACCAACGCGAAGTCGGTTTTGACACAAAATCGTA
TGCTAATGGATTAAAGGGGGCACTTCGCCAAGACCCAGATGTCATTCTTGTCGGTGAGATGCGTGATTTAGAAACGATGT
CAATCGCAATCACAGCAGCAGAAACAGGACATCTTGTGTTAGGAACACTCCATACTTCAAGTGCGCCAACAACCATCAAC
CGTATCATTGATGTCTTTCCATCAGGACAGCAGCCGCAAATACGGACCCAGCTTGCTTCGGTATTGGTTGGCGTCATTTC
GCAGCGCTTATTTCAGACAGCAGATAGAAAAGGCAGAAAAGCAGCAACAGAAATACTAGTCAACAATCCTGCGATTGCCA
ATTTAATTCGAAATGAAAAAATACACCAAATCCAAAGTACGATGCAAACGTCCCGTGCTCAGGGAATGCATACGTTAGAA
ATGAGCATTAAAGATTTACTGGATCGCGGTCTTATTCACAATGAAGCGGCAGCACAATATTTGCATGAAAAGATGATGTT
AAATGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.744

93.983

0.496
  pilT Vibrio cholerae strain A1552

48.837

98.567

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.837

98.567

0.481
  pilT Neisseria gonorrhoeae MS11

48.961

96.562

0.473
  pilT Neisseria meningitidis 8013

48.665

96.562

0.47
  pilT Acinetobacter baylyi ADP1

48.494

95.129

0.461
  pilT Pseudomonas aeruginosa PAK

48.466

93.41

0.453
  pilT Acinetobacter baumannii D1279779

47.447

95.415

0.453
  pilT Acinetobacter nosocomialis M2

47.447

95.415

0.453
  pilT Acinetobacter baumannii strain A118

47.447

95.415

0.453
  pilT Pseudomonas stutzeri DSM 10701

48.16

93.41

0.45
  pilT Legionella pneumophila strain Lp02

45.345

95.415

0.433
  pilT Legionella pneumophila strain ERS1305867

45.345

95.415

0.433
  pilU Pseudomonas stutzeri DSM 10701

42.515

95.702

0.407
  pilU Acinetobacter baylyi ADP1

42.331

93.41

0.395
  pilU Vibrio cholerae strain A1552

41.975

92.837

0.39