Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QNH29_RS23325 Genome accession   NZ_CP126093
Coordinates   4704002..4705051 (-) Length   349 a.a.
NCBI ID   WP_283861675.1    Uniprot ID   A0AA95L4X7
Organism   Neobacillus sp. DY30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4699002..4710051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH29_RS23300 (QNH29_23300) - 4699558..4700337 (-) 780 WP_283861671.1 PilN domain-containing protein -
  QNH29_RS23305 (QNH29_23305) pilM 4700337..4701332 (-) 996 WP_283861672.1 pilus assembly protein PilM -
  QNH29_RS23310 (QNH29_23310) - 4701349..4702110 (-) 762 WP_283861673.1 A24 family peptidase -
  QNH29_RS23315 (QNH29_23315) - 4702148..4702654 (-) 507 WP_349654870.1 type II secretion system protein -
  QNH29_RS23320 (QNH29_23320) - 4702795..4704009 (-) 1215 WP_283861674.1 type II secretion system F family protein -
  QNH29_RS23325 (QNH29_23325) pilT 4704002..4705051 (-) 1050 WP_283861675.1 type IV pilus twitching motility protein PilT Machinery gene
  QNH29_RS23330 (QNH29_23330) - 4705062..4706723 (-) 1662 WP_283861676.1 ATPase, T2SS/T4P/T4SS family -
  QNH29_RS23335 (QNH29_23335) - 4706748..4708046 (-) 1299 WP_283861677.1 hypothetical protein -
  QNH29_RS23340 (QNH29_23340) - 4708073..4709035 (-) 963 WP_283861679.1 hypothetical protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38783.91 Da        Isoelectric Point: 7.5086

>NTDB_id=835484 QNH29_RS23325 WP_283861675.1 4704002..4705051(-) (pilT) [Neobacillus sp. DY30]
MREKIDNILRAAIEFKASDIHLTVGVPPIFRINGDIKRYGKDILLPDDTEEIAKLTIPESMYDLFKEKGELDYSYGVPGV
SRFRCNAYHQRKSVSLALRVVASKTPTIEDLKLPPIIPQLCEKPQGLILVTGPTGSGKSTTLASMIDYMNRTMRKHIITL
EDPIEYLHKHGNCIIDQREVGFDTKSYANGLKGALRQDPDVILVGEMRDLETIGIAITAAETGHLVFGTLHTSSAPATIN
RIIDVFPPEQQPQIRVQLASVLVGVISQRLFPTTDGKGRRAATEIMVNNPAIANLIRSEKIHQIQSTMQTSRAQGMHTLE
MSIKELIDKGIIQTEAATKYLQEKMMLDG

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=835484 QNH29_RS23325 WP_283861675.1 4704002..4705051(-) (pilT) [Neobacillus sp. DY30]
ATGAGAGAAAAAATCGATAATATACTGCGTGCAGCGATTGAATTTAAAGCATCGGATATCCACTTAACTGTTGGTGTGCC
CCCGATTTTCCGTATAAACGGGGACATTAAACGCTATGGTAAAGACATTCTACTTCCAGATGATACGGAAGAAATAGCAA
AATTAACGATTCCTGAAAGTATGTATGATTTATTTAAAGAAAAAGGCGAGCTTGACTATTCCTATGGAGTTCCAGGGGTA
TCCCGTTTCCGTTGCAATGCGTATCATCAAAGAAAAAGTGTATCACTAGCACTAAGGGTGGTTGCATCAAAAACACCAAC
GATTGAGGACTTAAAGTTGCCGCCAATTATCCCACAGCTTTGTGAAAAACCACAGGGGCTAATTTTAGTTACTGGTCCAA
CGGGGAGCGGGAAATCTACTACGCTTGCTTCTATGATTGATTATATGAACCGGACCATGCGAAAGCACATTATTACATTA
GAGGACCCAATTGAATATCTGCATAAGCATGGCAATTGCATTATAGATCAGCGTGAAGTTGGTTTTGATACGAAGTCCTA
TGCAAATGGCCTTAAGGGTGCATTACGTCAAGACCCTGATGTCATTCTTGTCGGTGAGATGCGTGACCTTGAAACAATTG
GGATTGCGATCACTGCTGCGGAAACAGGGCACCTTGTTTTCGGGACGCTCCATACTTCGAGTGCTCCTGCAACTATAAAC
CGTATTATCGATGTTTTTCCACCAGAACAGCAGCCACAAATCCGTGTTCAGTTGGCTTCAGTTTTAGTAGGAGTAATTTC
ACAGCGTTTATTTCCTACAACAGATGGAAAAGGGCGTCGTGCTGCTACTGAAATTATGGTAAATAATCCGGCAATTGCGA
ACTTAATCCGAAGTGAAAAAATACATCAAATTCAAAGCACCATGCAAACTTCCCGTGCCCAGGGTATGCACACACTTGAA
ATGAGTATTAAAGAATTAATTGACAAAGGAATAATTCAAACGGAAGCTGCGACCAAATATCTACAAGAAAAGATGATGCT
TGATGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.524

93.983

0.484
  pilT Acinetobacter baylyi ADP1

48.83

97.994

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.384

98.567

0.467
  pilT Vibrio cholerae strain A1552

47.384

98.567

0.467
  pilT Neisseria gonorrhoeae MS11

47.522

98.281

0.467
  pilT Pseudomonas aeruginosa PAK

49.24

94.269

0.464
  pilT Pseudomonas stutzeri DSM 10701

49.846

93.123

0.464
  pilT Neisseria meningitidis 8013

47.23

98.281

0.464
  pilT Acinetobacter baumannii strain A118

48.348

95.415

0.461
  pilT Acinetobacter baumannii D1279779

48.348

95.415

0.461
  pilT Acinetobacter nosocomialis M2

48.348

95.415

0.461
  pilT Legionella pneumophila strain Lp02

46.334

97.708

0.453
  pilT Legionella pneumophila strain ERS1305867

46.334

97.708

0.453
  pilU Pseudomonas stutzeri DSM 10701

42.216

95.702

0.404
  pilU Acinetobacter baylyi ADP1

40.95

96.562

0.395
  pilU Vibrio cholerae strain A1552

40.123

92.837

0.372