Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VNTUMSATTG_RS16610 Genome accession   NZ_AP024087
Coordinates   3626294..3627331 (-) Length   345 a.a.
NCBI ID   WP_004398311.1    Uniprot ID   A0AAV2VQ50
Organism   Vibrio nigripulchritudo strain TUMSAT-TG-2018     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3621294..3632331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNTUMSATTG_RS16580 (VNTUMSATTG_32390) - 3621529..3622230 (+) 702 WP_200766203.1 endonuclease -
  VNTUMSATTG_RS16585 (VNTUMSATTG_32400) rsmE 3622336..3623067 (+) 732 WP_022593808.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VNTUMSATTG_RS16590 (VNTUMSATTG_32410) gshB 3623082..3624029 (+) 948 WP_022549509.1 glutathione synthase -
  VNTUMSATTG_RS16595 (VNTUMSATTG_32420) - 3624056..3624619 (+) 564 WP_004409298.1 YqgE/AlgH family protein -
  VNTUMSATTG_RS16600 (VNTUMSATTG_32430) ruvX 3624672..3625097 (+) 426 WP_022591347.1 Holliday junction resolvase RuvX -
  VNTUMSATTG_RS16605 (VNTUMSATTG_32440) pilU 3625179..3626285 (-) 1107 WP_004398310.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VNTUMSATTG_RS16610 (VNTUMSATTG_32450) pilT 3626294..3627331 (-) 1038 WP_004398311.1 type IV pilus twitching motility protein PilT Machinery gene
  VNTUMSATTG_RS16615 (VNTUMSATTG_32460) - 3627355..3628065 (+) 711 WP_004398312.1 YggS family pyridoxal phosphate-dependent enzyme -
  VNTUMSATTG_RS16620 (VNTUMSATTG_32470) proC 3628126..3628944 (+) 819 WP_004398313.1 pyrroline-5-carboxylate reductase -
  VNTUMSATTG_RS16625 (VNTUMSATTG_32480) - 3628974..3629531 (+) 558 WP_004398314.1 YggT family protein -
  VNTUMSATTG_RS16630 (VNTUMSATTG_32490) yggU 3629531..3629821 (+) 291 WP_004398315.1 DUF167 family protein YggU -
  VNTUMSATTG_RS16635 (VNTUMSATTG_32500) - 3629845..3630276 (+) 432 WP_200766204.1 DUF4426 domain-containing protein -
  VNTUMSATTG_RS16640 (VNTUMSATTG_32510) - 3630343..3630942 (+) 600 WP_022549504.1 XTP/dITP diphosphatase -
  VNTUMSATTG_RS16645 (VNTUMSATTG_32520) hemW 3630949..3632124 (+) 1176 WP_200766205.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38005.26 Da        Isoelectric Point: 6.2687

>NTDB_id=83397 VNTUMSATTG_RS16610 WP_004398311.1 3626294..3627331(-) (pilT) [Vibrio nigripulchritudo strain TUMSAT-TG-2018]
MDIAELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLGVPAFTHADVHRLVFDIMNDAQRAEFEEKLETDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTEVPSLEQISAPDIFTQISNYEKGLVLVTGPTGSGKSTTLAAMVDHINRNHNKHILTIED
PIEFVHQSNKCLINQREVHRDTHSFNAALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGTDKDMVRSMLSESLRAVISQKLLKRLGGGRAACHEIMLATPAIRNLIREDKVAQMYSVIQTGGAMGMQTTEQHA
KQLIAQGVVDADEVRTKLESDSMGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=83397 VNTUMSATTG_RS16610 WP_004398311.1 3626294..3627331(-) (pilT) [Vibrio nigripulchritudo strain TUMSAT-TG-2018]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCTGGAGTTCCTCCAAT
GGTCCGCGTAGACGGCGATGTACGTAAGTTGGGCGTACCTGCTTTTACTCATGCTGATGTTCACCGGTTGGTTTTCGATA
TTATGAACGACGCGCAACGCGCTGAGTTCGAAGAAAAACTGGAAACGGACTTCTCTTTTGAACTGCCTAATGTGGGTCGC
TTCCGTGTGAACGCTTTTAATCAGTCACGCGGGTGCTCGGCGGTATTTCGAACCATTCCAACCGAAGTGCCAAGCCTGGA
GCAAATCAGTGCGCCTGATATTTTTACTCAAATTTCTAACTATGAGAAAGGGTTAGTGCTGGTTACCGGTCCTACGGGTT
CGGGTAAATCCACCACACTTGCCGCCATGGTTGACCACATCAACCGCAATCACAACAAACACATTCTTACGATCGAAGAT
CCGATTGAGTTTGTACACCAAAGTAATAAATGCCTGATCAACCAACGTGAAGTTCATCGTGATACGCACAGCTTTAATGC
AGCGCTACGAAGCGCACTGCGTGAAGACCCGGATGTGATTCTGGTGGGTGAGCTTCGTGACAAGGAAACCATCAGCCTTG
CATTGACGGCTGCGGAAACGGGTCATTTGGTGTTTGGTACGTTGCACACCAGCAGTGCAGCCAAAACCGTCGACCGTATT
ATCGATGTGTTCCCGGGCACCGACAAAGACATGGTTCGATCAATGCTCTCTGAATCGCTACGTGCGGTTATTTCTCAAAA
GCTGCTTAAGCGTTTGGGTGGTGGGCGTGCTGCCTGTCATGAAATCATGCTGGCAACACCTGCGATTCGAAACCTGATTC
GTGAAGACAAAGTCGCTCAGATGTATTCGGTTATCCAGACAGGTGGCGCCATGGGGATGCAGACCACAGAGCAGCATGCT
AAACAGCTGATTGCTCAAGGGGTGGTCGATGCTGACGAGGTACGCACCAAACTTGAATCCGATTCTATGGGCTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

85.797

100

0.858
  pilT Vibrio cholerae strain A1552

85.797

100

0.858
  pilT Acinetobacter baumannii D1279779

72.917

97.391

0.71
  pilT Acinetobacter baumannii strain A118

72.917

97.391

0.71
  pilT Acinetobacter nosocomialis M2

72.619

97.391

0.707
  pilT Acinetobacter baylyi ADP1

70

98.551

0.69
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Pseudomonas aeruginosa PAK

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

69.301

95.362

0.661
  pilT Legionella pneumophila strain ERS1305867

69.301

95.362

0.661
  pilT Neisseria meningitidis 8013

63.689

100

0.641
  pilT Neisseria gonorrhoeae MS11

63.401

100

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.599

96.812

0.49
  pilU Vibrio cholerae strain A1552

41.846

94.203

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment