Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NSMS1_RS20690 Genome accession   NZ_AP023441
Coordinates   4772525..4773649 (+) Length   374 a.a.
NCBI ID   WP_224095295.1    Uniprot ID   -
Organism   Nostoc sp. MS1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4767525..4778649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSMS1_RS20680 (NSMS1_40590) grpE 4769290..4770033 (-) 744 WP_224086629.1 nucleotide exchange factor GrpE -
  NSMS1_RS20685 (NSMS1_40600) pilB/pilB1 4770436..4772448 (+) 2013 WP_224086630.1 GspE/PulE family protein Machinery gene
  NSMS1_RS20690 (NSMS1_40610) pilT 4772525..4773649 (+) 1125 WP_224095295.1 type IV pilus twitching motility protein PilT Machinery gene
  NSMS1_RS20695 (NSMS1_40620) - 4773726..4774937 (+) 1212 WP_224086631.1 type II secretion system F family protein -
  NSMS1_RS20700 (NSMS1_40630) - 4774937..4775491 (+) 555 WP_224095296.1 hypothetical protein -
  NSMS1_RS20705 (NSMS1_40640) - 4775574..4776083 (-) 510 WP_224086632.1 YceD family protein -
  NSMS1_RS20710 (NSMS1_40650) - 4776199..4776744 (-) 546 WP_224086633.1 protein jag -
  NSMS1_RS20715 (NSMS1_40660) yidC 4776748..4777890 (-) 1143 WP_224086634.1 membrane protein insertase YidC -
  NSMS1_RS20720 (NSMS1_40670) - 4778102..4778485 (-) 384 WP_224086635.1 PH domain-containing protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40956.38 Da        Isoelectric Point: 7.0974

>NTDB_id=83259 NSMS1_RS20690 WP_224095295.1 4772525..4773649(+) (pilT) [Nostoc sp. MS1]
MEMMIEDLMEQMIEMGGSDLHLSAGLPPYFRISGKLTPIGEEVLTADQCQRLIFSMLNNSQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREMCDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSASQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRIMAQEIMIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYKAGIISFEAAMSKTSKPDEIQRLIGGSAPPPAAGAKPGAVKAH

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=83259 NSMS1_RS20690 WP_224095295.1 4772525..4773649(+) (pilT) [Nostoc sp. MS1]
ATGGAAATGATGATAGAAGATTTGATGGAGCAAATGATTGAAATGGGTGGTTCGGATCTACACCTATCCGCAGGTTTACC
GCCTTATTTCCGCATTAGTGGGAAGCTTACACCAATAGGTGAAGAAGTACTCACTGCTGACCAATGCCAAAGGCTGATTT
TTAGTATGCTCAATAACAGCCAACGCAAAACCTTAGAACAAACTTGGGAGTTAGATTGTTCTTATGGTGTCAAGGGTTTG
GCTCGCTTCCGGGTGAATGTGTATAAAGAGCGTGGTGCTTACGCAGCTTGTTTACGGGCGCTGAGTTCTAAAATTCCTAA
CTTTGAAAAATTGGGTTTGCCTGATGTAGTACGGGAAATGTGCGACAAACCTAGAGGTTTGATTCTCGTAACCGGGCCAA
CAGGTTCTGGTAAAACGACCACTCTAGCGGCAATGATTGATTTAATTAACCGCACTAAAGCAGAGCATATTCTTACTGTA
GAAGACCCCATTGAATTTGTTTACGAACCAATTAAAAGCTTAGTTCACCAACGTCAATTAGGTGAAGATACAAAAAGTTT
TGCTAATGCTTTAAAAGCCGCGTTACGGGAAGATCCAGATATTATTCTGGTGGGTGAAATGCGTGATTTGGAAACAATTT
CCTTGGCAATTTCCGCAGCAGAAACCGGACACTTAGTCTTTGGGACACTACACACCAGTTCCGCCTCGCAAACTGTTGAC
CGGATTATTGACGTTTTCCCTCATGAAAGACAAACTCAAGTGCGTGTGCAATTATCTAACTCTTTAGTAGCGGTATTTAG
CCAAACTTTAGTACCTAAGAAAAACCCCAAACCGGGTGAATATGGTCGCATCATGGCGCAGGAAATTATGATTGTTACCC
CTGCTATTTCTAACCTAATTCGTGAAGGGAAAACGTCTCAAATTTACTCAGCAATTCAAACTGGTGGTAAGTTGGGGATG
CAAACTTTAGAGAAGGTTTTAGCTGATTATTACAAAGCAGGAATTATCTCTTTTGAAGCTGCGATGTCTAAAACCTCTAA
ACCAGATGAAATTCAACGTCTCATTGGTGGTTCTGCTCCACCACCCGCCGCAGGAGCTAAACCCGGAGCAGTCAAAGCGC
ACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.728

93.316

0.511
  pilT Pseudomonas stutzeri DSM 10701

54.155

93.316

0.505
  pilT Legionella pneumophila strain Lp02

53.314

92.781

0.495
  pilT Acinetobacter baylyi ADP1

53.314

92.781

0.495
  pilT Legionella pneumophila strain ERS1305867

53.314

92.781

0.495
  pilT Acinetobacter baumannii strain A118

52.161

92.781

0.484
  pilT Acinetobacter nosocomialis M2

52.161

92.781

0.484
  pilT Acinetobacter baumannii D1279779

52.161

92.781

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.956

98.128

0.471
  pilT Neisseria gonorrhoeae MS11

48.424

93.316

0.452
  pilT Vibrio cholerae strain A1552

49.415

91.444

0.452
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.444

0.452
  pilT Neisseria meningitidis 8013

48.138

93.316

0.449
  pilU Pseudomonas stutzeri DSM 10701

38.527

94.385

0.364
  pilU Acinetobacter baylyi ADP1

38.244

94.385

0.361

Multiple sequence alignment