Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NSMS1_RS20685 Genome accession   NZ_AP023441
Coordinates   4770436..4772448 (+) Length   670 a.a.
NCBI ID   WP_224086630.1    Uniprot ID   -
Organism   Nostoc sp. MS1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4765436..4777448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSMS1_RS20670 (NSMS1_40570) dnaJ 4765479..4766606 (-) 1128 WP_224086627.1 molecular chaperone DnaJ -
  NSMS1_RS20675 (NSMS1_40580) dnaK 4767011..4768981 (-) 1971 WP_224086628.1 molecular chaperone DnaK -
  NSMS1_RS20680 (NSMS1_40590) grpE 4769290..4770033 (-) 744 WP_224086629.1 nucleotide exchange factor GrpE -
  NSMS1_RS20685 (NSMS1_40600) pilB/pilB1 4770436..4772448 (+) 2013 WP_224086630.1 GspE/PulE family protein Machinery gene
  NSMS1_RS20690 (NSMS1_40610) pilT 4772525..4773649 (+) 1125 WP_224095295.1 type IV pilus twitching motility protein PilT Machinery gene
  NSMS1_RS20695 (NSMS1_40620) - 4773726..4774937 (+) 1212 WP_224086631.1 type II secretion system F family protein -
  NSMS1_RS20700 (NSMS1_40630) - 4774937..4775491 (+) 555 WP_224095296.1 hypothetical protein -
  NSMS1_RS20705 (NSMS1_40640) - 4775574..4776083 (-) 510 WP_224086632.1 YceD family protein -
  NSMS1_RS20710 (NSMS1_40650) - 4776199..4776744 (-) 546 WP_224086633.1 protein jag -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74826.65 Da        Isoelectric Point: 5.2545

>NTDB_id=83258 NSMS1_RS20685 WP_224086630.1 4770436..4772448(+) (pilB/pilB1) [Nostoc sp. MS1]
MTQSSPQRRSTALTTRTEFSPFGTKLVQSGYVNTEQMRQALIESRKSGRPLTEVLESITGRQLSPEFLRQYKKQQLFELK
ILYGVESLDPEVNSVGNTMVGNLIETLIPVDICRRHRLVPLSRNDEHNPPYVLVAMVSPDNLEASDDLNRILRPQGLSLQ
RMVITQEDYQQLINQYLDEMAVRQKHLEQEKFTDINQDLENLGNLDLEDAPDEMEADLGSAMKGAEDAPVINLVNRILAK
ALHEKVSDIHVEPQEENMRIRFRKDGVLREAFPPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVCLRILDNSSTQLGLDKLITDPETLNIVKDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLMRRVCGECKIAYNPTPEELARYGMTASGEVSVTFYKANTVPSEAIAEAKAKNELCPACNG
AGYRGRCGVYEVMKISENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKAKR
KSGLTCRTCTAQLQPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=83258 NSMS1_RS20685 WP_224086630.1 4770436..4772448(+) (pilB/pilB1) [Nostoc sp. MS1]
ATGACTCAATCGTCACCACAACGGCGCAGCACCGCTCTAACTACGAGAACAGAGTTTTCACCTTTCGGTACTAAATTAGT
ACAATCTGGCTACGTTAATACCGAACAGATGAGACAAGCGCTGATTGAAAGTCGCAAGTCTGGTAGACCCTTAACAGAAG
TTTTGGAGTCTATCACTGGACGACAGCTATCACCTGAGTTCCTCAGACAATATAAGAAACAGCAATTATTTGAATTAAAA
ATCCTATACGGTGTTGAATCTCTCGATCCAGAGGTCAACAGTGTTGGTAACACGATGGTGGGTAATTTAATTGAAACCCT
CATCCCAGTGGATATCTGCCGCCGTCACCGTTTAGTACCATTATCTAGAAATGATGAACATAACCCGCCCTATGTGTTAG
TGGCGATGGTTTCTCCAGATAATTTGGAAGCGTCGGATGACTTAAACCGCATCTTGCGCCCTCAAGGTTTATCATTACAG
CGTATGGTAATTACCCAGGAAGATTACCAACAGCTAATTAACCAATATTTGGATGAAATGGCTGTACGGCAAAAGCACCT
GGAACAAGAAAAATTTACAGATATTAATCAGGATTTAGAAAATCTCGGCAATCTCGACCTAGAAGATGCGCCAGACGAGA
TGGAAGCTGATTTAGGTTCAGCAATGAAAGGTGCGGAAGATGCGCCGGTCATTAATCTTGTCAATAGAATTTTGGCAAAA
GCATTGCACGAGAAGGTTTCTGATATCCATGTGGAACCGCAAGAAGAAAATATGCGTATTCGCTTCCGCAAGGATGGTGT
ACTAAGGGAGGCTTTTCCACCTCTACCGAAAAAAATTATCCCTGCGGTAACGGCGAGATTTAAAATTATTTCTAACTTAG
ATATTGCCGAGCGACGCTTACCCCAAGATGGACGTATTCGCCGGATGTTTGAAGGACGTAAGGTAGACTTCCGAGTAAAT
ACCTTACCTAGCCGCTATGGAGAAAAGGTTTGTCTGCGGATTTTGGACAACTCTTCTACCCAATTGGGCTTAGATAAGTT
GATTACTGACCCAGAAACTTTAAATATTGTTAAAGATATGGTCAGTAAGCCCTTCGGCTTGATTCTGGTAACGGGGCCGA
CTGGTTCAGGTAAGACGACTTCGCTTTATTCAGCGCTATCGGAGAAGAACTCTCCAGGGATTAACATTAGTACGGTAGAA
GACCCGATTGAATATAGTTTGCCAGGTATTACCCAGGTACAGGTAATTCGGGAAAAGGGTCTGGATTTTGCTACGGCGTT
ACGAGCATTTTTGCGACAAGACCCGGATGTACTACTGGTGGGTGAAACGCGAGATAAGGAAACAGCGAAAACAGCCATTG
AAGCGGCTTTAACGGGTCACTTGGTATTAACTACATTACATACCAACGATGCCCCTGGCGCGATCGCTCGTTTGGGAGAA
ATGGGTATTGAACCATTCATGGTTTCCAGTTCTCTGATTGGGGTATTGGCACAACGTCTCATGCGGCGTGTATGTGGTGA
GTGTAAGATTGCTTACAACCCCACACCCGAAGAACTTGCCCGTTATGGGATGACTGCTTCTGGTGAAGTGTCGGTTACAT
TCTATAAGGCTAACACCGTACCATCAGAAGCGATCGCTGAAGCTAAAGCCAAAAATGAGCTTTGCCCAGCTTGCAATGGT
GCTGGCTACAGAGGACGTTGCGGTGTTTATGAAGTCATGAAGATTTCCGAAAATCTGCAAACTCTCATCAACGAAGAAGC
ACCTACAGAACGTATCAAAGAAGTAGCTGTAGAAGAAGGGATGAAAACCTTGTTGGCTTATAGCTTAGACCTAGTGCGCC
AAGGTTCCACCACCTTAGAAGAAGTAGAACGGGTGACATTCACAGACACAGGTTTAGAAGCTGAGTTAAAAGCCAAACGC
AAGAGTGGTCTTACCTGTCGAACTTGTACTGCCCAACTACAACCAGAATGGCTAGATTGTCCCTACTGTATGACACCGAG
ATTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.476

100

0.657
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.636

95.97

0.361

Multiple sequence alignment