Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P9841_RS00970 Genome accession   NZ_CP125655
Coordinates   215135..216310 (+) Length   391 a.a.
NCBI ID   WP_283320265.1    Uniprot ID   -
Organism   Cellulomonas sp. ES6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 210135..221310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P9841_RS00950 (P9841_00950) ruvX 210426..210989 (+) 564 WP_283320261.1 Holliday junction resolvase RuvX -
  P9841_RS00955 (P9841_00955) mltG 210986..212149 (+) 1164 WP_283320262.1 endolytic transglycosylase MltG -
  P9841_RS00960 (P9841_00960) - 212146..213039 (+) 894 WP_283320263.1 shikimate dehydrogenase -
  P9841_RS00965 (P9841_00965) - 213157..214830 (+) 1674 WP_283320264.1 ATPase, T2SS/T4P/T4SS family -
  P9841_RS00970 (P9841_00970) pilT 215135..216310 (+) 1176 WP_283320265.1 type IV pilus twitching motility protein PilT Machinery gene
  P9841_RS00975 (P9841_00975) - 216310..217536 (+) 1227 WP_283320266.1 type II secretion system F family protein -
  P9841_RS00980 (P9841_00980) - 217698..218099 (+) 402 WP_283320267.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P9841_RS00985 (P9841_00985) - 218177..218605 (+) 429 WP_283320268.1 hypothetical protein -
  P9841_RS00990 (P9841_00990) - 218602..219186 (+) 585 WP_283320269.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P9841_RS00995 (P9841_00995) - 219177..221057 (+) 1881 WP_283320270.1 hypothetical protein -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 42175.92 Da        Isoelectric Point: 6.8705

>NTDB_id=832106 P9841_RS00970 WP_283320265.1 215135..216310(+) (pilT) [Cellulomonas sp. ES6]
MGIQRVDGDLDLDGALAAMVQAGASDLHLTSGAAPTIRLAGALQPLEGFGGLEAESLRRTLYSVLTQKQREKFEADLELD
MSYSVRGLARFRVNLYQQRESLGAAFRVIPYEIKPLEQLGVPPVVANFAGLPRGLVLVTGPTGSGKSTTLAAIIDLANRT
RADHIMTVEDPIEFLHRHKRSLINQREVGQDTHSFASALKHVLRQDPDIILVGELRDLETISVALTAAETGHLVFATLHT
QDAAQTIDRIIDVFPAHQQAQVRTQLAGALQGVVCQTLCKRSDAQARVVATEVLIATSAVRNLIREGKTHQIYSAMQAGA
QQGMHTLDQHLAELVKQGRISYETGLEKSHHSEDFNRLTGRFSGGVQGSATVRADAPVFGSPVYGVGGGAF

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=832106 P9841_RS00970 WP_283320265.1 215135..216310(+) (pilT) [Cellulomonas sp. ES6]
ATGGGCATCCAGCGCGTCGACGGCGACCTGGATCTCGACGGAGCGCTGGCGGCGATGGTGCAAGCTGGCGCATCGGACCT
GCATCTCACCTCGGGCGCGGCACCGACGATCCGGCTCGCAGGTGCCCTGCAGCCCCTCGAGGGCTTCGGCGGCCTGGAGG
CCGAGTCGCTGCGCCGCACGCTGTACTCGGTCCTGACCCAGAAGCAGCGCGAGAAGTTCGAGGCCGACCTCGAGCTCGAC
ATGTCGTACTCCGTACGAGGGCTCGCCCGCTTCCGGGTCAACCTGTACCAGCAGCGTGAGTCGCTCGGCGCCGCCTTCCG
CGTCATCCCGTACGAGATCAAGCCGCTCGAACAGCTCGGCGTCCCGCCCGTGGTGGCGAACTTCGCCGGTCTGCCCCGTG
GGCTCGTGCTGGTCACCGGGCCGACAGGCTCCGGCAAGTCCACCACGCTCGCTGCCATCATCGATCTGGCGAACCGGACC
CGAGCCGACCACATCATGACGGTGGAAGACCCCATCGAGTTCCTCCACCGGCACAAGCGATCGCTCATCAACCAGCGTGA
GGTGGGACAGGACACGCACTCGTTCGCGTCGGCGCTGAAGCACGTGCTCCGGCAGGACCCGGACATCATCCTCGTCGGGG
AGCTGCGGGACCTGGAGACGATCTCGGTTGCTCTCACCGCGGCCGAGACCGGCCACCTCGTGTTCGCAACTCTCCACACG
CAGGACGCTGCTCAGACCATCGACCGCATCATCGACGTGTTCCCGGCCCACCAGCAGGCGCAGGTGCGGACCCAGCTCGC
CGGCGCGCTCCAGGGCGTGGTGTGCCAGACGCTGTGCAAGCGCTCCGATGCGCAGGCGCGGGTGGTCGCGACGGAGGTCC
TGATCGCCACGTCCGCCGTGCGGAACCTGATCCGCGAGGGCAAGACGCACCAGATCTACTCCGCGATGCAGGCCGGTGCG
CAGCAGGGCATGCACACGCTCGACCAGCACCTGGCGGAGCTGGTGAAGCAGGGACGGATCTCCTACGAGACCGGCCTGGA
GAAGAGCCACCACAGCGAGGACTTCAACCGCCTGACCGGGAGGTTCTCGGGCGGTGTCCAGGGATCGGCAACCGTGCGCG
CGGACGCGCCCGTGTTCGGTTCCCCGGTCTACGGGGTCGGGGGAGGTGCCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.133

95.908

0.481
  pilT Acinetobacter baumannii strain A118

53.179

88.491

0.471
  pilT Acinetobacter nosocomialis M2

53.179

88.491

0.471
  pilT Acinetobacter baumannii D1279779

53.179

88.491

0.471
  pilT Acinetobacter baylyi ADP1

52.89

88.491

0.468
  pilT Pseudomonas aeruginosa PAK

52.89

88.491

0.468
  pilT Legionella pneumophila strain Lp02

52.616

87.98

0.463
  pilT Legionella pneumophila strain ERS1305867

52.616

87.98

0.463
  pilT Pseudomonas stutzeri DSM 10701

51.445

88.491

0.455
  pilT Neisseria gonorrhoeae MS11

50.733

87.212

0.442
  pilT Neisseria meningitidis 8013

50.733

87.212

0.442
  pilT Vibrio cholerae strain A1552

49.848

84.143

0.419
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.848

84.143

0.419
  pilU Pseudomonas stutzeri DSM 10701

41.908

88.491

0.371
  pilU Vibrio cholerae strain A1552

39.56

93.095

0.368
  pilU Acinetobacter baylyi ADP1

40.286

89.514

0.361