Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U0530_RS25240 Genome accession   NZ_CP139853
Coordinates   5209839..5210819 (-) Length   326 a.a.
NCBI ID   WP_074447042.1    Uniprot ID   -
Organism   Escherichia coli strain Nord122     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5204839..5215819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0530_RS25210 (U0530_25210) yggI 5205504..5206001 (+) 498 Protein_4927 SprT family zinc-dependent metalloprotease -
  U0530_RS25215 (U0530_25215) endA 5206096..5206803 (+) 708 WP_023149797.1 deoxyribonuclease I -
  U0530_RS25220 (U0530_25220) rsmE 5206883..5207614 (+) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  U0530_RS25225 (U0530_25225) gshB 5207627..5208577 (+) 951 WP_000593273.1 glutathione synthase -
  U0530_RS25230 (U0530_25230) yqgE 5208686..5209249 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  U0530_RS25235 (U0530_25235) ruvX 5209249..5209665 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  U0530_RS25240 (U0530_25240) pilT 5209839..5210819 (-) 981 WP_074447042.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U0530_RS25245 (U0530_25245) yggS 5210837..5211541 (+) 705 WP_000997804.1 pyridoxal phosphate homeostasis protein -
  U0530_RS25250 (U0530_25250) yggT 5211559..5212125 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  U0530_RS25255 (U0530_25255) yggU 5212122..5212412 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  U0530_RS25260 (U0530_25260) rdgB 5212420..5213013 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  U0530_RS25265 (U0530_25265) hemW 5213006..5214142 (+) 1137 WP_000239953.1 radical SAM family heme chaperone HemW -
  U0530_RS25270 (U0530_25270) yggM 5214267..5215214 (-) 948 WP_000745231.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36027.22 Da        Isoelectric Point: 5.8348

>NTDB_id=832026 U0530_RS25240 WP_074447042.1 5209839..5210819(-) (pilT) [Escherichia coli strain Nord122]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAYILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAGLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=832026 U0530_RS25240 WP_074447042.1 5209839..5210819(-) (pilT) [Escherichia coli strain Nord122]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGTATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGGGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

31.481

100

0.365