Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QFW87_RS27675 Genome accession   NZ_CP125618
Coordinates   6026045..6027079 (-) Length   344 a.a.
NCBI ID   WP_008152619.1    Uniprot ID   -
Organism   Pseudomonas sp. PMCC200344     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6021045..6032079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QFW87_RS27645 ruvX 6021272..6021709 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  QFW87_RS27650 pyrR 6021793..6022296 (+) 504 WP_033060467.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  QFW87_RS27655 - 6022324..6023328 (+) 1005 WP_007897943.1 aspartate carbamoyltransferase catalytic subunit -
  QFW87_RS27660 - 6023325..6024596 (+) 1272 WP_008152614.1 dihydroorotase -
  QFW87_RS27665 - 6024693..6025124 (-) 432 WP_283182027.1 TM2 domain-containing protein -
  QFW87_RS27670 - 6025374..6025994 (+) 621 WP_283182028.1 C40 family peptidase -
  QFW87_RS27675 pilT 6026045..6027079 (-) 1035 WP_008152619.1 type IV pilus twitching motility protein PilT Machinery gene
  QFW87_RS27680 - 6027135..6027833 (+) 699 WP_283182030.1 YggS family pyridoxal phosphate-dependent enzyme -
  QFW87_RS27685 proC 6027867..6028685 (+) 819 WP_283182031.1 pyrroline-5-carboxylate reductase -
  QFW87_RS27690 - 6028696..6029286 (+) 591 WP_283182032.1 YggT family protein -
  QFW87_RS27695 - 6029291..6029587 (+) 297 WP_283189331.1 DUF167 domain-containing protein -
  QFW87_RS27700 - 6029821..6030939 (-) 1119 WP_283182018.1 IS481 family transposase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37959.61 Da        Isoelectric Point: 7.2157

>NTDB_id=831933 QFW87_RS27675 WP_008152619.1 6026045..6027079(-) (pilT) [Pseudomonas sp. PMCC200344]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVQALIYDIMNDTQRVDFEKHLETDFSFDVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLSMDDLGMGEVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDHLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KELVSKGLISREHAREKARTPDGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=831933 QFW87_RS27675 WP_008152619.1 6026045..6027079(-) (pilT) [Pseudomonas sp. PMCC200344]
ATGGATATCACTGAGCTGCTGGCTTTCAGCGCGAAACAGGGCGCGTCCGACTTGCATCTGTCTGCCGGCCTGCCACCGAT
GATTCGGGTCGACGGCGATGTGCGGCGCATCAACCTGCCGGCCTTGGACCACAAGCAGGTGCAGGCGCTGATCTACGACA
TCATGAACGACACGCAGCGGGTGGACTTCGAAAAGCACCTGGAGACCGACTTCTCCTTTGACGTGCCCGGCGTGGCGCGT
TTTCGGGTTAACGCGTTCAACCACAATCGCGGTGCTGGCGCGGTGTTCCGGACCATTCCGTCCAAGGTCCTGAGCATGGA
CGACCTCGGTATGGGGGAGGTGTTTCGCAAGATTACCGAAGCGCCGCGGGGGCTGGTGCTGGTGACCGGTCCGACCGGTT
CCGGCAAATCCACCACCCTGGCGGCGATGATCGATCACCTGAACACCCATCGTCATCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTCCACGAGTCGCGCAAATGTTTGATCAACCAGCGTGAAGTCCATCGCGATACACGCAGCTTCGCCAC
GGCGCTGCGCTCGGCCCTGCGGGAGGACCCGGACGTGATTCTGGTGGGGGAGATGCGGGATCTCGAGACCATTCGCCTGG
CGTTGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACGCTACACACGACATCAGCGGCGAAAACTATCGACCGGGTG
GTGGACGTGTTTCCGGGGGACGAGAAGTCGATGGTTCGTTCGATGCTTTCCGAGTCGTTACTGGCGGTGGTGTCGCAGAC
GTTGGTCAAGAAAATCGGCGGCGGGCGGATCGCGGCCCACGAAATCATGCTCGGCACGTCGGCGATCCGTAACCTGATCC
GCGAAGATAAAGTGGCGCAGATGTACTCGTCGATTCAGACCGGGGGAAACCTGGGGATGCAGACGCTGGATATGTGCTTG
AAGGAGCTGGTGAGCAAGGGCTTGATCAGCCGCGAGCATGCGCGGGAGAAGGCGCGCACGCCGGATGGTTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

86.337

100

0.863
  pilT Pseudomonas aeruginosa PAK

85.465

100

0.855
  pilT Acinetobacter nosocomialis M2

78.198

100

0.782
  pilT Acinetobacter baumannii D1279779

77.907

100

0.779
  pilT Acinetobacter baumannii strain A118

77.907

100

0.779
  pilT Acinetobacter baylyi ADP1

76.163

100

0.762
  pilT Legionella pneumophila strain Lp02

70.93

100

0.709
  pilT Legionella pneumophila strain ERS1305867

70.93

100

0.709
  pilT Neisseria meningitidis 8013

65.507

100

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.965

0.657
  pilT Vibrio cholerae strain A1552

67.062

97.965

0.657
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.65

99.709

0.395
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392