Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMK55_RS11440 Genome accession   NZ_CP125378
Coordinates   2522865..2523899 (-) Length   344 a.a.
NCBI ID   WP_102358360.1    Uniprot ID   A0A2N7R3R8
Organism   Pseudomonas sp. P8_229     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2517865..2528899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK55_RS11410 (QMK55_11405) ruvX 2517952..2518389 (+) 438 WP_003229101.1 Holliday junction resolvase RuvX -
  QMK55_RS11415 (QMK55_11410) pyrR 2518470..2518976 (+) 507 WP_102358364.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  QMK55_RS11420 (QMK55_11415) - 2519004..2520008 (+) 1005 WP_102358363.1 aspartate carbamoyltransferase catalytic subunit -
  QMK55_RS11425 (QMK55_11420) - 2520005..2521276 (+) 1272 WP_102358362.1 dihydroorotase -
  QMK55_RS11430 (QMK55_11425) - 2521429..2521860 (-) 432 WP_102358361.1 NINE protein -
  QMK55_RS11435 (QMK55_11430) - 2522111..2522737 (+) 627 WP_003229112.1 C40 family peptidase -
  QMK55_RS11440 (QMK55_11435) pilT 2522865..2523899 (-) 1035 WP_102358360.1 type IV pilus twitching motility protein PilT Machinery gene
  QMK55_RS11445 (QMK55_11440) - 2523956..2524642 (+) 687 WP_102358359.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMK55_RS11450 (QMK55_11445) proC 2524676..2525494 (+) 819 WP_102358358.1 pyrroline-5-carboxylate reductase -
  QMK55_RS11455 (QMK55_11450) - 2525505..2526095 (+) 591 WP_102358357.1 YggT family protein -
  QMK55_RS11460 (QMK55_11455) - 2526092..2526379 (+) 288 WP_320329206.1 DUF167 domain-containing protein -
  QMK55_RS11465 (QMK55_11460) metX 2526558..2527697 (+) 1140 WP_320329207.1 homoserine O-succinyltransferase MetX -
  QMK55_RS11470 (QMK55_11465) metW 2527705..2528325 (+) 621 WP_003229126.1 methionine biosynthesis protein MetW -
  QMK55_RS11475 (QMK55_11470) - 2528352..2528786 (+) 435 WP_102358354.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38103.80 Da        Isoelectric Point: 7.0158

>NTDB_id=831622 QMK55_RS11440 WP_102358360.1 2522865..2523899(-) (pilT) [Pseudomonas sp. P8_229]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KELMTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=831622 QMK55_RS11440 WP_102358360.1 2522865..2523899(-) (pilT) [Pseudomonas sp. P8_229]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGTGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACACCTCGAAACGGATTTCTCGTTCGAAGTCCCCGGCGTGGCGCGT
TTTCGGGTCAATGCGTTCAACCAGAACCGTGGTGCCGGGGCGGTGTTCCGCACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGGCATGGGCGATGTCTTTCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGTT
CCGGCAAGTCGACCACGCTGGCGGCGATGATCGATTACCTCAACACCCATCGACATCACCATATCCTCACCATTGAAGAC
CCGATCGAATTCGTCCACGAATCGCGCAAATGCCTGATCAACCAGCGCGAGGTACACCGCGACACCCGCAGCTTTGCCAC
CGCGCTGCGTTCGGCGCTGCGCGAAGACCCGGACGTGATTCTGGTGGGTGAGATGCGTGATCTGGAAACCATTCGCCTGG
CGCTGACTGCCGCCGAAACCGGGCATCTGGTGTTCGGCACGCTGCACACCACTTCAGCGGCGAAAACCATCGACCGGGTG
GTGGATGTATTCCCGGGGGATGAGAAGTCGATGGTGCGTTCGATGCTCTCGGAGTCGTTGCTGGCAGTGGTCTCGCAGAC
CCTGATCAAGAAGATCGGCGGCGGACGGGTGGCGGCGCACGAGATCATGCTGGGCACATCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACCGGCGGGTCGTTGGGGATGCAGACACTCGACATGTGCCTG
AAGGAGCTGATGACCAAAGGCTTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGTACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7R3R8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

39.42

100

0.395