Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMK52_RS09010 Genome accession   NZ_CP125376
Coordinates   2046488..2047522 (+) Length   344 a.a.
NCBI ID   WP_005792266.1    Uniprot ID   -
Organism   Pseudomonas sp. P9_2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2041488..2052522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK52_RS08980 (QMK52_08980) - 2041833..2042267 (-) 435 WP_005792275.1 DUF4426 domain-containing protein -
  QMK52_RS08985 (QMK52_08985) metW 2042289..2042909 (-) 621 WP_005792273.1 methionine biosynthesis protein MetW -
  QMK52_RS08990 (QMK52_08990) metX 2042917..2044056 (-) 1140 WP_005792271.1 homoserine O-succinyltransferase MetX -
  QMK52_RS08995 (QMK52_08995) - 2044278..2044865 (-) 588 WP_003195087.1 YggT family protein -
  QMK52_RS09000 (QMK52_09000) proC 2044891..2045709 (-) 819 WP_005792268.1 pyrroline-5-carboxylate reductase -
  QMK52_RS09005 (QMK52_09005) - 2045735..2046430 (-) 696 WP_005792267.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMK52_RS09010 (QMK52_09010) pilT 2046488..2047522 (+) 1035 WP_005792266.1 type IV pilus twitching motility protein PilT Machinery gene
  QMK52_RS09015 (QMK52_09015) - 2047671..2048309 (-) 639 WP_005792265.1 C40 family peptidase -
  QMK52_RS09020 (QMK52_09020) - 2048559..2048987 (+) 429 WP_320404022.1 TM2 domain-containing protein -
  QMK52_RS09025 (QMK52_09025) - 2049107..2050378 (-) 1272 WP_005792263.1 dihydroorotase -
  QMK52_RS09030 (QMK52_09030) - 2050375..2051379 (-) 1005 WP_005792261.1 aspartate carbamoyltransferase catalytic subunit -
  QMK52_RS09035 (QMK52_09035) pyrR 2051404..2051910 (-) 507 WP_005792260.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  QMK52_RS09040 (QMK52_09040) ruvX 2052043..2052480 (-) 438 WP_005792259.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37484.42 Da        Isoelectric Point: 7.2665

>NTDB_id=831566 QMK52_RS09010 WP_005792266.1 2046488..2047522(+) (pilT) [Pseudomonas sp. P9_2]
MDITQLLTASVSRGACDLHLSAGLAPMLRVDGEVWPMDEPVLNAAQLVDLVSPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQNRGIGAVFRAIPTEVQSLEDLGLGEVFQRMAQFPRGLVLVTGPTGSGKSTTLAAMIDYLNRHRRQHILTLED
PIEFIHRPKLALITQRQVHRDTHSFCVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGLVSREEAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=831566 QMK52_RS09010 WP_005792266.1 2046488..2047522(+) (pilT) [Pseudomonas sp. P9_2]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGTGGCGCCTGTGACCTGCATTTGTCGGCCGGCCTGGCGCCGAT
GCTGCGAGTCGATGGCGAGGTCTGGCCGATGGATGAGCCGGTGCTGAACGCAGCGCAACTGGTCGATCTGGTGAGCCCTT
TGTTGAATAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAATTGCCGGGAGTCGCACGC
TTTCGGGCGAACGTGTTTCGCCAGAATCGCGGTATAGGCGCGGTGTTTCGCGCCATCCCGACCGAGGTCCAGAGCCTGGA
AGACCTTGGGTTGGGCGAGGTCTTCCAGCGTATGGCTCAGTTCCCCCGTGGCCTGGTGCTGGTCACCGGGCCTACGGGTT
CGGGCAAGTCCACGACGCTGGCGGCGATGATCGATTATCTGAACCGGCACCGGCGCCAGCACATCCTCACGCTTGAAGAC
CCCATCGAATTTATCCACAGGCCGAAACTGGCCCTGATCACCCAACGCCAGGTGCACCGCGACACCCACAGCTTCTGCGT
GGCCCTGCGCTCGGCCCTGAGGGAAGACCCTGATGTGATCCTGGTAGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CCTTGACGGCAGCTGAGACCGGGCACCTGGTGTTTGGCACCCTGCACACTTCATCGGCTGCAAAGACTGTAGACAGGCTG
GTGGACGTCTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCGATGTTGTCGGAGTCGTTGCAGGCGGTGGTGTCTCAGGT
ACTGGTGAAGAAGGTCGGCGGCGGCCGTGTGGCCGCCCATGAAATCATGCTGGGCACGCCGGCGATTCGCAATCTGATCC
GTGAGGACAAGGTGGCGCAGATGGTTTCGGCAATTCAGACCGGTGGCGCGTTGGGGATGAAGACGCTGGATATGAGTTTG
AAGGCGTTGGTCGGCGCGGGGCTGGTCAGCCGGGAAGAGGCGCGGGAGAAGGCGAGGGTGCCTGCAGATATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.093

100

0.721
  pilT Pseudomonas stutzeri DSM 10701

70.349

100

0.703
  pilT Acinetobacter baumannii D1279779

68.915

99.128

0.683
  pilT Acinetobacter baumannii strain A118

68.915

99.128

0.683
  pilT Acinetobacter nosocomialis M2

68.915

99.128

0.683
  pilT Acinetobacter baylyi ADP1

67.742

99.128

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.798

97.965

0.625
  pilT Vibrio cholerae strain A1552

63.798

97.965

0.625
  pilT Legionella pneumophila strain Lp02

63.609

98.256

0.625
  pilT Legionella pneumophila strain ERS1305867

63.609

98.256

0.625
  pilT Neisseria meningitidis 8013

61.176

98.837

0.605
  pilT Neisseria gonorrhoeae MS11

60.882

98.837

0.602
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

100

0.488
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381