Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMK51_RS02050 Genome accession   NZ_CP125375
Coordinates   451450..452484 (+) Length   344 a.a.
NCBI ID   WP_320393024.1    Uniprot ID   -
Organism   Pseudomonas sp. P9_31     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446450..457484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK51_RS02020 (QMK51_02020) metW 446909..447529 (-) 621 WP_027926558.1 methionine biosynthesis protein MetW -
  QMK51_RS02025 (QMK51_02025) metX 447537..448676 (-) 1140 WP_320393023.1 homoserine O-succinyltransferase MetX -
  QMK51_RS02030 (QMK51_02030) - 448956..449249 (-) 294 WP_258698646.1 DUF167 domain-containing protein -
  QMK51_RS02035 (QMK51_02035) - 449254..449844 (-) 591 WP_258698647.1 YggT family protein -
  QMK51_RS02040 (QMK51_02040) proC 449855..450673 (-) 819 WP_258698648.1 pyrroline-5-carboxylate reductase -
  QMK51_RS02045 (QMK51_02045) - 450707..451393 (-) 687 WP_258698649.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMK51_RS02050 (QMK51_02050) pilT 451450..452484 (+) 1035 WP_320393024.1 type IV pilus twitching motility protein PilT Machinery gene
  QMK51_RS02055 (QMK51_02055) - 452534..453154 (-) 621 WP_320393025.1 C40 family peptidase -
  QMK51_RS02060 (QMK51_02060) - 453405..453836 (+) 432 WP_320393026.1 TM2 domain-containing protein -
  QMK51_RS02065 (QMK51_02065) - 453947..455218 (-) 1272 WP_258698653.1 dihydroorotase -
  QMK51_RS02070 (QMK51_02070) - 455215..456219 (-) 1005 WP_007897943.1 aspartate carbamoyltransferase catalytic subunit -
  QMK51_RS02075 (QMK51_02075) pyrR 456247..456750 (-) 504 WP_258698654.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  QMK51_RS02080 (QMK51_02080) ruvX 456840..457286 (-) 447 WP_018928712.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37984.66 Da        Isoelectric Point: 7.0157

>NTDB_id=831529 QMK51_RS02050 WP_320393024.1 451450..452484(+) (pilT) [Pseudomonas sp. P9_31]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKAVHDLIYDIMNDLQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGEVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNSHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRIAAHEIMLGTAAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KELVTKGVISREHAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=831529 QMK51_RS02050 WP_320393024.1 451450..452484(+) (pilT) [Pseudomonas sp. P9_31]
ATGGATATCACTGAGCTGCTGGCTTTCAGCGCTAAACAGGGCGCGTCCGACCTGCACCTGTCCGCCGGCCTGCCACCGAT
GATTCGTGTCGACGGCGATGTGAGGCGCATCAACCTGCCGGCGCTGGACCACAAGGCGGTGCATGATCTGATCTACGACA
TCATGAACGACCTCCAGCGGGTGGACTTCGAGAAGCATCTTGAAACCGACTTTTCCTTCGAAGTGCCCGGCGTGGCGCGT
TTTCGGGTCAATGCCTTCAACCAGAATCGCGGTGCGGGCGCGGTATTCCGGACCATTCCGTCCAAGGTCCTGAGCATGGA
CGACCTGGGCATGGGGGAAGTGTTTCGCAAGATTACCGACGCGCCCCGGGGCCTGGTGCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCCACCACGCTGGCAGCGATGATCGATTACCTGAACAGCCATCGCCATCACCACATCCTCACCATCGAAGAC
CCCATCGAGTTCGTCCACGAATCGCGTAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGCTTCGCCAC
TGCCCTGCGCTCTGCCCTGCGCGAAGACCCGGACGTGATTCTGGTGGGGGAGATGCGTGATCTGGAGACTATCCGCTTGG
CGTTGACTGCCGCCGAGACCGGCCACCTGGTGTTTGGCACACTGCACACCACGTCGGCGGCGAAAACCATCGACCGGGTG
GTGGACGTGTTTCCGGGCGACGAGAAATCGATGGTGCGTTCGATGCTCTCCGAGTCATTGCTGGCGGTGGTGTCCCAGAC
ATTGATCAAGAAAATCGGCGGCGGGCGGATTGCCGCCCACGAAATCATGCTCGGCACCGCGGCGATTCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGAGGGTCGCTGGGGATGCAGACGCTGGATATGTGCCTG
AAAGAACTGGTGACCAAGGGCGTGATCAGCCGCGAACATGCGCGGGAGAAGGCGCGGTCGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Pseudomonas aeruginosa PAK

86.919

100

0.869
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

72.093

100

0.721
  pilT Legionella pneumophila strain ERS1305867

72.093

100

0.721
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

65.507

100

0.657
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.401
  pilU Vibrio cholerae strain A1552

40

100

0.401