Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PMI20_RS01110 Genome accession   NZ_CP124732
Coordinates   249506..250540 (-) Length   344 a.a.
NCBI ID   WP_007929982.1    Uniprot ID   -
Organism   Pseudomonas sp. GM17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 244506..255540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMI20_RS01080 (PMI20_001080) ruvX 244657..245094 (+) 438 WP_007929974.1 Holliday junction resolvase RuvX -
  PMI20_RS01085 (PMI20_001085) pyrR 245216..245722 (+) 507 WP_007929976.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PMI20_RS01090 (PMI20_001090) - 245749..246753 (+) 1005 WP_007929978.1 aspartate carbamoyltransferase catalytic subunit -
  PMI20_RS01095 (PMI20_001095) - 246750..248021 (+) 1272 WP_007929979.1 dihydroorotase -
  PMI20_RS01100 (PMI20_001100) - 248127..248546 (-) 420 WP_007929980.1 TM2 domain-containing protein -
  PMI20_RS01105 (PMI20_001105) - 248797..249429 (+) 633 WP_007929981.1 C40 family peptidase -
  PMI20_RS01110 (PMI20_001110) pilT 249506..250540 (-) 1035 WP_007929982.1 type IV pilus twitching motility protein PilT Machinery gene
  PMI20_RS01115 (PMI20_001115) - 250598..251296 (+) 699 WP_007929983.1 YggS family pyridoxal phosphate-dependent enzyme -
  PMI20_RS01120 (PMI20_001120) proC 251331..252149 (+) 819 WP_007929984.1 pyrroline-5-carboxylate reductase -
  PMI20_RS01125 (PMI20_001125) - 252160..252750 (+) 591 WP_007929986.1 YggT family protein -
  PMI20_RS01130 (PMI20_001130) - 252764..253057 (+) 294 WP_007929988.1 DUF167 domain-containing protein -
  PMI20_RS01135 (PMI20_001135) - 253267..254406 (+) 1140 WP_007921181.1 homoserine O-acetyltransferase -
  PMI20_RS01140 (PMI20_001140) metW 254414..255034 (+) 621 WP_007921185.1 methionine biosynthesis protein MetW -
  PMI20_RS01145 (PMI20_001145) - 255062..255496 (+) 435 WP_007921193.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37554.25 Da        Isoelectric Point: 7.1605

>NTDB_id=827774 PMI20_RS01110 WP_007929982.1 249506..250540(-) (pilT) [Pseudomonas sp. GM17]
MDITELLAFSVKQGASDLHLSAGLPPMIRIDGDIRRINLPVLEAQQVHELILASMTDPQRAEFKAALEIDFSFSVPGLAR
FRVNAFNHQRGGGAVFRSIAAKVPSLDELGMGEVFRRISEVPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRHQHILTIED
PIEFIHESKKCLINQREVHRDTRSFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRV
VDVFPAEEKAMVRSMLSESLQAVVSQALVKRIGGGRVAAHEIMLGSAAIRNLIREDKVAQMYSAIQTGGALGMQTLDMSL
KTLVDAGLVSREHAREKARAPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=827774 PMI20_RS01110 WP_007929982.1 249506..250540(-) (pilT) [Pseudomonas sp. GM17]
ATGGATATCACTGAGTTGCTGGCCTTCAGCGTCAAACAGGGCGCTTCGGACCTGCACCTCTCGGCCGGTTTGCCGCCGAT
GATTCGTATCGACGGCGATATTCGCCGGATCAACCTGCCGGTGCTGGAGGCGCAACAGGTGCACGAGCTGATCCTGGCCA
GCATGACTGATCCGCAGCGGGCCGAGTTCAAGGCCGCGCTGGAGATCGACTTTTCCTTCTCGGTGCCGGGTCTGGCGCGC
TTTCGGGTCAATGCCTTCAACCATCAGCGCGGCGGTGGCGCGGTCTTTCGCAGCATTGCGGCCAAGGTACCGAGCCTGGA
CGAACTGGGCATGGGCGAGGTGTTTCGCCGGATCAGCGAAGTGCCCCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGATCGATCACCTGAATCAGCACCGGCACCAGCACATCCTCACCATCGAGGAC
CCCATCGAGTTCATCCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACCCGCAGCTTCTCCAC
GGCCCTGCGCTCGGCCCTGCGCGAAGACCCGGATGTGATTCTGGTGGGGGAAATGCGCGATCTGGAAACCATCCGCTTGG
CGCTGACCGCCGCGGAGACCGGGCATCTGGTGTTCGGCACCCTGCACACCACGTCGGCGGCGAAAACCGTGGACCGGGTG
GTGGACGTGTTTCCGGCCGAGGAGAAGGCGATGGTTCGCTCCATGCTCTCGGAGTCGCTGCAGGCGGTGGTTTCCCAGGC
GCTGGTGAAGCGTATCGGCGGAGGCCGGGTAGCGGCTCACGAGATCATGCTGGGCAGCGCGGCGATCCGTAACCTGATCC
GCGAGGACAAAGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGGGCGCTGGGGATGCAGACCCTGGACATGAGCCTG
AAGACGCTGGTGGATGCGGGGTTGGTCAGCCGGGAGCACGCCCGGGAAAAGGCGCGGGCGCCGGACAGTATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

80.523

100

0.805
  pilT Pseudomonas stutzeri DSM 10701

77.616

100

0.776
  pilT Acinetobacter nosocomialis M2

72.965

100

0.73
  pilT Acinetobacter baumannii D1279779

72.965

100

0.73
  pilT Acinetobacter baumannii strain A118

72.965

100

0.73
  pilT Acinetobacter baylyi ADP1

72.093

100

0.721
  pilT Legionella pneumophila strain Lp02

67.456

98.256

0.663
  pilT Legionella pneumophila strain ERS1305867

67.456

98.256

0.663
  pilT Neisseria meningitidis 8013

64.928

100

0.651
  pilT Neisseria gonorrhoeae MS11

64.638

100

0.648
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.965

0.645
  pilT Vibrio cholerae strain A1552

65.875

97.965

0.645
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.877

99.419

0.506
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392