Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SE698_RS17450 Genome accession   NZ_CP138428
Coordinates   3647523..3648503 (-) Length   326 a.a.
NCBI ID   WP_319054045.1    Uniprot ID   -
Organism   Escherichia coli strain 6EVA     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3642523..3653503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE698_RS17420 yggI 3643186..3643683 (+) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -
  SE698_RS17425 endA 3643778..3644485 (+) 708 WP_000286500.1 deoxyribonuclease I -
  SE698_RS17430 rsmE 3644565..3645296 (+) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SE698_RS17435 gshB 3645309..3646259 (+) 951 WP_000593274.1 glutathione synthase -
  SE698_RS17440 yqgE 3646368..3646931 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  SE698_RS17445 ruvX 3646931..3647347 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  SE698_RS17450 pilT 3647523..3648503 (-) 981 WP_319054045.1 type IV pilus twitching motility protein PilT Machinery gene
  SE698_RS17455 yggS 3648521..3649225 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  SE698_RS17460 yggT 3649243..3649809 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  SE698_RS17465 yggU 3649806..3650096 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  SE698_RS17470 rdgB 3650104..3650697 (+) 594 WP_001174752.1 XTP/dITP diphosphatase -
  SE698_RS17475 hemW 3650690..3651826 (+) 1137 WP_000239939.1 radical SAM family heme chaperone HemW -
  SE698_RS17480 yggM 3652068..3653075 (-) 1008 WP_000745221.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35972.15 Da        Isoelectric Point: 5.7980

>NTDB_id=826478 SE698_RS17450 WP_319054045.1 3647523..3648503(-) (pilT) [Escherichia coli strain 6EVA]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENSLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=826478 SE698_RS17450 WP_319054045.1 3647523..3648503(-) (pilT) [Escherichia coli strain 6EVA]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGTTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATAGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362