Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QI031_RS28090 Genome accession   NZ_CP124543
Coordinates   6411030..6412154 (-) Length   374 a.a.
NCBI ID   WP_281482845.1    Uniprot ID   A0AAJ6P9D3
Organism   Halotia branconii CENA392     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6406030..6417154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QI031_RS28060 (QI031_28060) - 6406624..6406947 (-) 324 WP_281482840.1 type II toxin-antitoxin system PemK/MazF family toxin -
  QI031_RS28065 (QI031_28065) - 6406941..6407201 (-) 261 WP_281482841.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  QI031_RS28070 (QI031_28070) - 6407473..6408495 (+) 1023 WP_281482842.1 saccharopine dehydrogenase-like oxidoreductase -
  QI031_RS28075 (QI031_28075) - 6408564..6408941 (-) 378 WP_281482843.1 four helix bundle protein -
  QI031_RS28080 (QI031_28080) - 6408978..6409535 (-) 558 WP_281486144.1 hypothetical protein -
  QI031_RS28085 (QI031_28085) - 6409608..6410831 (-) 1224 WP_281482844.1 type II secretion system F family protein -
  QI031_RS28090 (QI031_28090) pilT 6411030..6412154 (-) 1125 WP_281482845.1 type IV pilus twitching motility protein PilT Machinery gene
  QI031_RS28095 (QI031_28095) pilB/pilB1 6412239..6414251 (-) 2013 WP_281482846.1 GspE/PulE family protein Machinery gene
  QI031_RS28100 (QI031_28100) grpE 6414782..6415528 (+) 747 WP_281482847.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40810.11 Da        Isoelectric Point: 7.4414

>NTDB_id=826381 QI031_RS28090 WP_281482845.1 6411030..6412154(-) (pilT) [Halotia branconii CENA392]
MEMMIEDLMEQMIEMGGSDLHLSAGLPPYFRISGKLTPIGDQVLSADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREMSDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVSKKNPKPGEYGRVMAQEILIVTPAVSNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADLYKAGTISFEAAMSKTSKPDEIQRLIGTAAPQAGAKPGVGAAKAH

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=826381 QI031_RS28090 WP_281482845.1 6411030..6412154(-) (pilT) [Halotia branconii CENA392]
ATGGAAATGATGATTGAAGACTTGATGGAGCAGATGATTGAAATGGGTGGCTCGGATTTACATTTATCCGCAGGTTTGCC
TCCCTACTTCCGTATCAGTGGCAAACTCACTCCCATAGGCGACCAAGTATTGAGTGCTGACCAGTGCCAAAGACTAATTT
TTAGTATGCTTAACAATACCCAGCGTAAAACCCTAGAGCAGACTTGGGAGTTGGATTGTTCTTATGGTGTTAAAGGTTTA
GCTCGTTTCCGGGTCAACGTCTATAAAGAACGTGGTGCTTATGCTGCTTGTTTACGGGCGTTAAGTTCTAAAATTCCTAA
CTTTGAAAAATTAGGTCTGCCAGATGTAGTGCGGGAAATGTCAGATAAACCCAGAGGACTGATTCTAGTGACAGGGCCTA
CTGGTTCTGGTAAAACTACTACCCTAGCGGCCATGATTGACTTGATTAACCGCACCAAAGCTGAGCATATCTTAACAGTA
GAAGACCCAATTGAATTTGTTTATGAACCCATCAAAAGTTTAGTCCACCAGCGACAGTTGGGTGAAGATACTAAAAGTTT
TGCTAATGCCTTAAAAGCAGCTCTACGGGAAGATCCAGACATTATTCTAGTGGGAGAAATGCGGGATTTGGAAACAATTT
CTTTGGCGATTTCGGCGGCGGAAACAGGACACTTGGTATTTGGTACACTGCATACCAGTTCTGCTGCACAAACCGTTGAC
CGGATTATTGATGTTTTCCCTCATGAAAGACAAACCCAAGTGCGGGTGCAGTTGTCTAACTCATTAGTGGCGGTATTTAG
CCAAACTTTGGTATCCAAGAAAAACCCGAAACCAGGTGAGTATGGTCGGGTAATGGCTCAAGAAATTCTGATTGTAACTC
CCGCTGTTTCTAACTTGATTCGAGAAGGTAAAACATCTCAAATCTACTCAGCAATTCAAACTGGTGGCAAATTGGGAATG
CAGACTTTAGAAAAAGTTTTGGCTGATTTGTACAAAGCCGGAACTATCTCTTTTGAAGCGGCAATGTCTAAAACTTCCAA
ACCAGATGAAATTCAACGTCTGATTGGTACTGCCGCACCACAAGCAGGTGCAAAACCCGGTGTAGGGGCAGCTAAAGCAC
ATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.301

93.316

0.516
  pilT Pseudomonas stutzeri DSM 10701

54.441

93.316

0.508
  pilT Legionella pneumophila strain Lp02

53.026

92.781

0.492
  pilT Acinetobacter baylyi ADP1

53.026

92.781

0.492
  pilT Legionella pneumophila strain ERS1305867

53.026

92.781

0.492
  pilT Acinetobacter baumannii strain A118

52.161

92.781

0.484
  pilT Acinetobacter nosocomialis M2

52.161

92.781

0.484
  pilT Acinetobacter baumannii D1279779

52.161

92.781

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.167

96.257

0.473
  pilT Neisseria gonorrhoeae MS11

51.622

90.642

0.468
  pilT Neisseria meningitidis 8013

51.327

90.642

0.465
  pilT Vibrio cholerae strain A1552

49.415

91.444

0.452
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.444

0.452
  pilU Pseudomonas stutzeri DSM 10701

38.781

96.524

0.374
  pilU Acinetobacter baylyi ADP1

38.81

94.385

0.366