Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   QI031_RS28095 Genome accession   NZ_CP124543
Coordinates   6412239..6414251 (-) Length   670 a.a.
NCBI ID   WP_281482846.1    Uniprot ID   A0AAJ6NS12
Organism   Halotia branconii CENA392     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6407239..6419251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QI031_RS28070 (QI031_28070) - 6407473..6408495 (+) 1023 WP_281482842.1 saccharopine dehydrogenase-like oxidoreductase -
  QI031_RS28075 (QI031_28075) - 6408564..6408941 (-) 378 WP_281482843.1 four helix bundle protein -
  QI031_RS28080 (QI031_28080) - 6408978..6409535 (-) 558 WP_281486144.1 hypothetical protein -
  QI031_RS28085 (QI031_28085) - 6409608..6410831 (-) 1224 WP_281482844.1 type II secretion system F family protein -
  QI031_RS28090 (QI031_28090) pilT 6411030..6412154 (-) 1125 WP_281482845.1 type IV pilus twitching motility protein PilT Machinery gene
  QI031_RS28095 (QI031_28095) pilB/pilB1 6412239..6414251 (-) 2013 WP_281482846.1 GspE/PulE family protein Machinery gene
  QI031_RS28100 (QI031_28100) grpE 6414782..6415528 (+) 747 WP_281482847.1 nucleotide exchange factor GrpE -
  QI031_RS28105 (QI031_28105) dnaK 6415813..6417774 (+) 1962 WP_281482848.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74953.71 Da        Isoelectric Point: 4.9969

>NTDB_id=826382 QI031_RS28095 WP_281482846.1 6412239..6414251(-) (pilB/pilB1) [Halotia branconii CENA392]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGFVNTDQMRQALIESRKSGRPLTEVLESLTGQQLSPEFLRQYKKQQLFELK
ILYGVEFLDPEVNDVGNTMVGSLIETLIPVDICRRHRLVPLSKNEDQNPPYVLVAMVDPDNLEASDDLNRILRPQNLALQ
RMVITQEDYQQLINQYLDELAVRQKHLEQEKFTDINQDLENLGSLDIQDAPEEMEADLGSAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIIANLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDMVAKPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSDCRVPYTPTVEELARYGLSASQDVGVTFYKANSLTLESIAEAKAKNQVCPTCNG
VGYKGRCGVYEVMRVTENLQTLINQEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKR
KSGLTCRTCDATLQPEWLDCPYCMTSRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=826382 QI031_RS28095 WP_281482846.1 6412239..6414251(-) (pilB/pilB1) [Halotia branconii CENA392]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACAAGAACAGAGTTTTCGCCCTTCGGCAACAAATTAGT
ACAGTCTGGTTTTGTCAATACCGACCAAATGAGACAGGCGCTGATTGAAAGTCGCAAGTCTGGCAGACCCCTGACGGAAG
TGTTGGAGTCGCTCACCGGACAGCAGCTATCACCTGAGTTCCTCAGGCAGTACAAGAAACAGCAGCTATTTGAACTTAAA
ATACTATACGGCGTTGAATTTCTCGATCCGGAAGTCAATGACGTTGGCAATACGATGGTGGGGAGCCTGATTGAAACCCT
GATCCCGGTAGATATTTGTCGTCGTCATCGCTTAGTGCCACTATCAAAAAATGAAGACCAAAACCCACCATACGTTTTGG
TGGCAATGGTCGACCCGGATAATCTAGAAGCTTCGGATGATTTGAACCGCATCTTGCGCCCCCAAAACTTGGCATTGCAG
CGCATGGTGATTACGCAAGAAGATTATCAACAGCTGATCAATCAATATTTAGATGAGTTGGCTGTGCGGCAAAAACATCT
GGAACAAGAAAAGTTTACAGATATTAATCAGGACTTAGAAAACCTCGGCAGTCTTGATATCCAAGACGCACCTGAGGAAA
TGGAAGCTGACCTTGGTTCAGCAATGAAGGGTGCAGAAGATGCCCCAGTCATCAACCTCGTCAACAGAATCCTGGCTAAA
GCTTTGCATGAGAAGGTTTCTGATATTCATATCGAACCGCAAGAAGAAAACTTACGCATTCGCTTTCGCAAGGATGGGGT
ATTGCGTGAAGCTTTCGATCCCCTGCCGAAAAAAATCATCCCAGCAGTGACAGCTCGATTTAAAATCATTGCTAACCTAG
ACATTGCCGAACGGCGTTTACCTCAAGACGGACGTATTCGGCGGATGTTTGAGGGACGTAAAGTGGATTTCCGGGTGAAT
ACTTTGCCCAGTCGCTACGGGGAAAAAGTAGTACTGCGGATTTTGGATAACTCCTCCACTCAATTGGGATTGAATAAGTT
AATTACCGATCCAGAGACTTTACAAATTGTCCAGGATATGGTAGCTAAACCTTTCGGTTTGATTTTGGTAACTGGACCGA
CTGGTTCAGGGAAAACAACCTCACTGTATTCAGCGCTCTCTGAAAAGAATGATCCTGGAATTAATATTAGTACTGTAGAA
GACCCGATTGAGTATAGCTTGCCAGGGATTACTCAAGTACAGGTGATTCGGGAAAAAGGACTGGATTTTGCGACGGCGTT
GCGGGCATTTTTGCGACAAGATCCGGATGTGCTGCTTGTAGGTGAAACGCGAGACAAAGAAACAGCAAAAACAGCAATTG
AGGCTGCTTTAACTGGTCACTTAGTATTAACTACCTTACATACTAATGATGCCCCAGGAGCGATCGCTCGTCTAGGAGAA
ATGGGCATTGAACCGTTCATGGTTTCCAGTTCCCTGATTGGGGTTTTAGCACAGCGGCTAGTGCGGCGTGTATGTTCCGA
TTGCCGTGTTCCCTATACCCCCACGGTGGAAGAACTAGCTCGCTATGGTCTATCTGCTTCTCAAGATGTGGGAGTAACCT
TCTACAAAGCTAACAGCCTGACATTAGAAAGCATTGCGGAAGCTAAAGCCAAAAATCAGGTATGTCCAACATGTAACGGC
GTTGGTTACAAAGGTCGCTGTGGTGTTTATGAAGTCATGCGCGTCACTGAAAACCTGCAAACTTTAATCAACCAAGAAGC
ACCAACAGAACGCATTAAAGAAGTAGCTGTGGAAGAAGGCATGAAAACCTTGTTGGCCTACAGTCTGGACTTAGTACGTC
AAGGTGCTACCACCCTTGAAGAAGTGGAACGGGTGACGTTTACCGACACAGGTTTAGAAGCCGAGCTAAAAGCCAAACGC
AAGAGTGGTCTAACCTGCCGAACTTGTGATGCCACACTACAACCAGAATGGCTGGATTGTCCTTATTGCATGACATCTCG
GTTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.774

100

0.66