Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QH639_RS23110 Genome accession   NZ_CP123966
Coordinates   4329926..4330972 (-) Length   348 a.a.
NCBI ID   WP_016991992.1    Uniprot ID   A0AAE8QS02
Organism   Lysinibacillus sp.     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4324926..4335972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QH639_RS23080 (QH639_23080) - 4325235..4325927 (-) 693 WP_131519508.1 hypothetical protein -
  QH639_RS23085 (QH639_23085) - 4325924..4326517 (-) 594 WP_281115337.1 hypothetical protein -
  QH639_RS23090 (QH639_23090) pilM 4326517..4327491 (-) 975 WP_131519506.1 pilus assembly protein PilM -
  QH639_RS23095 (QH639_23095) - 4327604..4328065 (-) 462 WP_131519505.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QH639_RS23100 (QH639_23100) - 4328122..4328598 (-) 477 WP_131519504.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QH639_RS23105 (QH639_23105) - 4328724..4329929 (-) 1206 WP_036080216.1 type II secretion system F family protein -
  QH639_RS23110 (QH639_23110) pilT 4329926..4330972 (-) 1047 WP_016991992.1 type IV pilus twitching motility protein PilT Machinery gene
  QH639_RS23115 (QH639_23115) - 4330984..4332648 (-) 1665 WP_103119054.1 GspE/PulE family protein -
  QH639_RS23120 (QH639_23120) - 4332672..4334000 (-) 1329 WP_131519503.1 G5 domain-containing protein -
  QH639_RS23125 (QH639_23125) - 4334538..4335002 (+) 465 WP_131519502.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QH639_RS23130 (QH639_23130) - 4335004..4335453 (+) 450 WP_131519501.1 type II secretion system protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38914.79 Da        Isoelectric Point: 7.0543

>NTDB_id=823338 QH639_RS23110 WP_016991992.1 4329926..4330972(-) (pilT) [Lysinibacillus sp.]
MTAYSIVELLTRAFEEKASDLHLTKGIPPVYRIHGQLEHYGTVPLETEDLKEMVKVILPVHKQQEFEDKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAKIPSIEALNMPQVLYKLAEKPQGLILVTGPTGSGKSTTLAAMIDHINETRSKHIIT
LEDPIEYLHTHKKSVINQREIGVDTMSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQSQIRVQLANVLQGIVSQRLFVRKDVKGRIAATEILVQAPAISNLIRNEKIHQIPSVMQTSKALGMHTL
EYSMQHLITTGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=823338 QH639_RS23110 WP_016991992.1 4329926..4330972(-) (pilT) [Lysinibacillus sp.]
ATGACAGCATATTCAATCGTTGAATTATTAACACGTGCATTTGAAGAAAAAGCATCTGACCTTCATTTAACGAAAGGTAT
TCCACCGGTATATCGTATCCATGGGCAACTGGAGCATTACGGCACAGTCCCACTTGAAACAGAGGATTTAAAGGAAATGG
TAAAGGTCATTCTTCCTGTCCATAAACAACAGGAATTTGAGGACAAGGGAGAAACGGACTTTAACTTTTCATTGCCTGGG
AAATGTCGATTCCGTGTCAATGCATACCATCAACGAAATGAAGTAACGATTGCAGCTCGTTTAATTGAGGCTAAAATTCC
TTCTATTGAAGCGCTCAATATGCCACAGGTGTTATATAAGCTTGCAGAAAAACCACAAGGCTTAATATTAGTGACAGGCC
CAACAGGCTCAGGGAAATCAACTACCTTGGCAGCCATGATAGATCATATCAATGAAACAAGATCTAAACATATTATTACG
TTAGAGGATCCAATTGAATATTTGCATACACATAAGAAGTCTGTCATCAATCAACGGGAGATTGGTGTCGATACAATGAG
CTTTGCCAATGGTTTACGTGCATCATTACGTCAGGATCCAGATATTATTTTAGTTGGGGAGATGCGAGATTATGAAACCA
TTTCAACGGCTATTACGGCTGCTGAAACAGGGCATTTAGTTATGGCTACACTGCATACAAGTAGTGCACCAACGACGATC
GACCGTATTATCGATGTATTTCCACCGCATCAGCAGAGTCAAATTCGTGTGCAGCTTGCGAATGTACTACAAGGGATTGT
TTCTCAGCGTTTATTTGTACGTAAGGATGTAAAAGGGCGTATTGCTGCGACAGAAATTTTAGTACAGGCACCTGCTATTT
CAAATTTAATTCGTAACGAAAAAATTCACCAAATTCCAAGTGTGATGCAGACAAGTAAAGCTTTGGGTATGCACACATTG
GAATATTCGATGCAGCACCTAATTACGACAGGTAAGATTTCACTAGAGGATGCAAGACCATATATGAACGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.083

96.552

0.503
  pilT Neisseria meningitidis 8013

50

95.977

0.48
  pilT Neisseria gonorrhoeae MS11

49.701

95.977

0.477
  pilT Acinetobacter baumannii D1279779

49.401

95.977

0.474
  pilT Acinetobacter nosocomialis M2

49.401

95.977

0.474
  pilT Acinetobacter baumannii strain A118

49.401

95.977

0.474
  pilT Pseudomonas aeruginosa PAK

49.102

95.977

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.503

95.977

0.466
  pilT Vibrio cholerae strain A1552

48.503

95.977

0.466
  pilT Legionella pneumophila strain Lp02

49.24

94.54

0.466
  pilT Legionella pneumophila strain ERS1305867

49.24

94.54

0.466
  pilT Pseudomonas stutzeri DSM 10701

48.204

95.977

0.463
  pilT Acinetobacter baylyi ADP1

47.904

95.977

0.46
  pilU Pseudomonas stutzeri DSM 10701

42.598

95.115

0.405
  pilU Vibrio cholerae strain A1552

42.236

92.529

0.391
  pilU Acinetobacter baylyi ADP1

40.06

95.402

0.382