Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DE22_RS03880 Genome accession   NZ_CP123660
Coordinates   805559..806539 (+) Length   326 a.a.
NCBI ID   WP_001055662.1    Uniprot ID   A0A3V9S2X2
Organism   Salmonella enterica subsp. enterica serovar Kentucky strain CVM N38232     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 800559..811539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DE22_RS03850 (DE22_03850) - 801135..802142 (+) 1008 WP_000252200.1 DUF1202 domain-containing protein -
  DE22_RS03855 (DE22_03855) hemW 802234..803371 (-) 1138 Protein_758 radical SAM family heme chaperone HemW -
  DE22_RS03860 (DE22_03860) - 803364..803957 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  DE22_RS03865 (DE22_03865) yggU 803965..804255 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  DE22_RS03870 (DE22_03870) - 804252..804818 (-) 567 WP_001094848.1 YggT family protein -
  DE22_RS03875 (DE22_03875) - 804837..805541 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  DE22_RS03880 (DE22_03880) pilT 805559..806539 (+) 981 WP_001055662.1 type IV pilus twitching motility protein PilT Machinery gene
  DE22_RS03885 (DE22_03885) - 806671..807357 (+) 687 WP_000098323.1 IclR family transcriptional regulator -
  DE22_RS03890 (DE22_03890) ruvX 807404..807820 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  DE22_RS03895 (DE22_03895) - 807820..808383 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  DE22_RS03900 (DE22_03900) gshB 808599..809546 (-) 948 WP_000593248.1 glutathione synthase -
  DE22_RS03905 (DE22_03905) rsmE 809566..810297 (-) 732 WP_001675718.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DE22_RS03910 (DE22_03910) endA 810374..811081 (-) 708 WP_000286114.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36140.34 Da        Isoelectric Point: 7.5355

>NTDB_id=821898 DE22_RS03880 WP_001055662.1 805559..806539(+) (pilT) [Salmonella enterica subsp. enterica serovar Kentucky strain CVM N38232]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDVQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=821898 DE22_RS03880 WP_001055662.1 805559..806539(+) (pilT) [Salmonella enterica subsp. enterica serovar Kentucky strain CVM N38232]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACAGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGTGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(55-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3V9S2X2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.245

97.853

0.374
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365
  pilU Pseudomonas stutzeri DSM 10701

35.65

100

0.362