Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QFB85_RS02770 Genome accession   NZ_CP123607
Coordinates   586662..587684 (+) Length   340 a.a.
NCBI ID   WP_280631215.1    Uniprot ID   -
Organism   Serratia marcescens strain RMCH-M16-N     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 581662..592684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QFB85_RS02730 (QFB85_02730) - 581961..582204 (+) 244 Protein_528 DUF4160 domain-containing protein -
  QFB85_RS02735 (QFB85_02735) - 582188..582436 (+) 249 WP_004937422.1 DUF2442 domain-containing protein -
  QFB85_RS02740 (QFB85_02740) hemW 582473..583615 (-) 1143 WP_048796774.1 radical SAM family heme chaperone HemW -
  QFB85_RS02745 (QFB85_02745) - 583608..584201 (-) 594 WP_154871791.1 XTP/dITP diphosphatase -
  QFB85_RS02750 (QFB85_02750) yggU 584220..584510 (-) 291 WP_004948014.1 DUF167 family protein YggU -
  QFB85_RS02755 (QFB85_02755) - 584507..585061 (-) 555 WP_004937436.1 YggT family protein -
  QFB85_RS02760 (QFB85_02760) proC 585093..585914 (-) 822 WP_004937440.1 pyrroline-5-carboxylate reductase -
  QFB85_RS02765 (QFB85_02765) - 585934..586641 (-) 708 WP_015379048.1 YggS family pyridoxal phosphate-dependent enzyme -
  QFB85_RS02770 (QFB85_02770) pilT 586662..587684 (+) 1023 WP_280631215.1 type IV pilus twitching motility protein PilT Machinery gene
  QFB85_RS02775 (QFB85_02775) ruvX 587681..588103 (-) 423 WP_074055092.1 Holliday junction resolvase RuvX -
  QFB85_RS02780 (QFB85_02780) - 588103..588666 (-) 564 WP_004937452.1 YqgE/AlgH family protein -
  QFB85_RS02785 (QFB85_02785) gshB 588800..589750 (-) 951 WP_016930084.1 glutathione synthase -
  QFB85_RS02790 (QFB85_02790) rsmE 589761..590492 (-) 732 WP_280631216.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QFB85_RS02795 (QFB85_02795) endA 590562..591257 (-) 696 WP_015379043.1 deoxyribonuclease I -
  QFB85_RS02800 (QFB85_02800) - 591359..591871 (-) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36371.71 Da        Isoelectric Point: 7.5735

>NTDB_id=821510 QFB85_RS02770 WP_280631215.1 586662..587684(+) (pilT) [Serratia marcescens strain RMCH-M16-N]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCNGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLLLVTGATGSGKSTTLAAMIDEINRHQPRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=821510 QFB85_RS02770 WP_280631215.1 586662..587684(+) (pilT) [Serratia marcescens strain RMCH-M16-N]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTTTGCAACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAGTCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGTGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCAGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAGGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGAGTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.453

97.353

0.491
  pilT Neisseria gonorrhoeae MS11

50.151

97.353

0.488
  pilT Legionella pneumophila strain ERS1305867

49.096

97.647

0.479
  pilT Legionella pneumophila strain Lp02

49.096

97.647

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.503

100

0.453
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.319

95

0.374
  pilU Pseudomonas stutzeri DSM 10701

38.509

94.706

0.365