Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P7V53_RS00620 Genome accession   NZ_CP123228
Coordinates   126430..127473 (-) Length   347 a.a.
NCBI ID   WP_280153539.1    Uniprot ID   -
Organism   Piscinibacter sp. XHJ-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 121430..132473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7V53_RS00590 - 122020..122388 (+) 369 WP_280153533.1 YraN family protein -
  P7V53_RS00595 - 122457..123053 (+) 597 WP_280153534.1 phosphoheptose isomerase -
  P7V53_RS00600 - 123050..123676 (+) 627 WP_280153535.1 BON domain-containing protein -
  P7V53_RS00605 - 123677..124573 (-) 897 WP_280153536.1 NAD(P)-dependent oxidoreductase -
  P7V53_RS00610 pilU 124632..125768 (-) 1137 WP_280153537.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P7V53_RS00615 - 125788..126417 (-) 630 WP_280153538.1 cyclic nucleotide-binding domain-containing protein -
  P7V53_RS00620 pilT 126430..127473 (-) 1044 WP_280153539.1 type IV pilus twitching motility protein PilT Machinery gene
  P7V53_RS00625 - 127536..128222 (+) 687 WP_280153540.1 YggS family pyridoxal phosphate-dependent enzyme -
  P7V53_RS00630 - 128250..129143 (-) 894 WP_280153541.1 DMT family transporter -
  P7V53_RS00635 - 129246..130154 (+) 909 WP_280153542.1 AraC family transcriptional regulator -
  P7V53_RS00640 - 130106..130588 (-) 483 WP_280153543.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38108.81 Da        Isoelectric Point: 7.0669

>NTDB_id=820093 P7V53_RS00620 WP_280153539.1 126430..127473(-) (pilT) [Piscinibacter sp. XHJ-5]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLEHKQVHDMVYDIMNDSQRKSYEETLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLAAPKVFADLALKPRGMVLVTGPTGSGKSTTLAAMVNHLNENEYGHVLTVED
PIEFVHESKKCLINQREVGPHTLSFANALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRAMLSESLVAVISQTLCKLKDGSGRVAAHEIMLGTAAIRNLIRENKVAQMYSAIQTGNSMGMQTLDQN
LADLVRRNIISPAEARGKAKFPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=820093 P7V53_RS00620 WP_280153539.1 126430..127473(-) (pilT) [Piscinibacter sp. XHJ-5]
ATGGACATCACCCAACTGCTGGCGTTCTCGGTCAAGAACAAGGCGTCGGACCTGCACCTGTCAGCCGGATTGCCCCCGAT
GATCCGGGTGCACGGCGACGTGCGCCGCATCAACGTCGACCCGCTCGAGCACAAGCAGGTGCACGACATGGTGTACGACA
TCATGAACGACTCGCAGCGCAAGTCGTACGAAGAGACGCTGGAGTGCGACTTCTCCTTCGAGATCCAGGGCCTCGCGCGC
TTTCGCGTCAACGCGTTCAATCAGAACCGCGGCGCCGGCGCGGTGTTCCGGACCATTCCGTCGAAGATCCTCACCCTCGA
GCAGCTGGCCGCACCCAAGGTGTTCGCCGACCTGGCCCTGAAGCCGCGCGGCATGGTCCTGGTGACCGGCCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCGCCATGGTGAACCACCTCAACGAGAACGAATACGGCCACGTGCTGACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGTCCGCACACGCTGAGCTTCGCCAA
TGCGCTGCGCTCGGCGCTGCGCGAGGATCCCGATGCCATCCTGGTCGGCGAAATGCGCGACCTCGAGACCATCCGCCTGG
CCCTCACGGCGGCGGAAACCGGGCACCTGGTGTTCGGCACGCTGCATACGTCAAGCGCCGCGAAGACGGTGGACCGCATC
GTCGACGTCTTCCCCGCGGCCGAGAAGGAGATGGTTCGCGCCATGCTGTCGGAGTCGCTGGTGGCGGTGATCTCCCAGAC
GCTGTGCAAGCTGAAGGACGGCTCCGGGCGAGTGGCGGCGCACGAGATCATGCTGGGCACCGCCGCCATCCGCAACCTCA
TCCGCGAGAACAAGGTCGCCCAGATGTACTCCGCGATCCAGACCGGCAACAGCATGGGAATGCAGACGCTCGACCAGAAC
CTTGCCGACCTGGTTCGCCGCAACATCATCTCCCCTGCGGAGGCGCGCGGCAAAGCCAAGTTCCCGGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

71.386

97.695

0.697
  pilT Legionella pneumophila strain ERS1305867

71.386

97.695

0.697
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.864

97.406

0.651
  pilT Vibrio cholerae strain A1552

66.864

97.406

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401