Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JMG82_RS03655 Genome accession   NZ_AP023212
Coordinates   737673..738746 (+) Length   357 a.a.
NCBI ID   WP_201353581.1    Uniprot ID   -
Organism   Hydrogenimonas urashimensis strain SSM-sur55     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 732673..743746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMG82_RS03615 - 732869..733561 (-) 693 WP_201353573.1 class I SAM-dependent methyltransferase -
  JMG82_RS03620 hisG 733551..734168 (-) 618 WP_201353574.1 ATP phosphoribosyltransferase -
  JMG82_RS03625 - 734162..734803 (-) 642 WP_201353575.1 type III pantothenate kinase -
  JMG82_RS03630 - 734782..735096 (-) 315 WP_201353576.1 hypothetical protein -
  JMG82_RS03635 - 735108..736346 (-) 1239 WP_201353577.1 hypothetical protein -
  JMG82_RS03640 - 736343..736957 (-) 615 WP_201353578.1 hypothetical protein -
  JMG82_RS03645 gatC 737038..737328 (+) 291 WP_201353579.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  JMG82_RS03650 - 737332..737676 (+) 345 WP_201353580.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JMG82_RS03655 pilT 737673..738746 (+) 1074 WP_201353581.1 type IV pilus twitching motility protein PilT Machinery gene
  JMG82_RS03660 - 738889..740241 (+) 1353 WP_201353582.1 3-deoxy-7-phosphoheptulonate synthase class II -
  JMG82_RS03665 - 740370..741305 (+) 936 WP_201353583.1 GGDEF domain-containing protein -
  JMG82_RS03670 - 741313..741903 (+) 591 WP_201353584.1 CvpA family protein -
  JMG82_RS03675 - 741900..742385 (+) 486 WP_201353585.1 Fur family transcriptional regulator -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39568.49 Da        Isoelectric Point: 6.7838

>NTDB_id=81665 JMG82_RS03655 WP_201353581.1 737673..738746(+) (pilT) [Hydrogenimonas urashimensis strain SSM-sur55]
MSNVALDIRKLLKNVVAYKASDLHLVSRSEPQIRIDGRLVPLNLPKLEGEAIEQMCYSIITEKQKKSFEEHKELDFAIQI
PEVGRFRANFYRTMGDVAAAFRVIPTHTPSLDDLEAPQVYKKLIQREKGLILVTGPTGSGKSTTLAAMLNEINMSERKHI
ITIEDPIEFVHENHQSLFSHRNIGTDTDSFATALKYALREDPDVILIGEMRDKETIAAALTAAETGHLVFGTVHTNSAAQ
TVNRIIDVFSGDEQPQIRAQLSTSLVAVISQALLPKIGGGRIAVPEILISNPAIANLIREDKVHQIYSQMQLGQGESGMQ
TQTQVMVKLVKGHIIEKSEALRFSTRPDELLKALQHG

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=81665 JMG82_RS03655 WP_201353581.1 737673..738746(+) (pilT) [Hydrogenimonas urashimensis strain SSM-sur55]
ATGAGCAATGTCGCACTCGATATCCGAAAACTTCTGAAAAATGTCGTCGCCTACAAAGCGAGCGACCTGCACCTCGTCAG
CCGCAGCGAACCGCAGATCCGTATCGACGGACGGCTCGTGCCCCTGAACCTTCCGAAACTCGAGGGGGAGGCGATCGAGC
AGATGTGCTACTCGATCATCACCGAGAAGCAGAAAAAGTCGTTCGAAGAGCACAAAGAGCTCGACTTCGCGATCCAGATT
CCCGAAGTGGGCCGGTTCCGTGCCAACTTCTACCGAACCATGGGTGACGTTGCGGCGGCGTTCCGTGTCATTCCGACCCA
TACCCCCAGCCTCGACGATCTCGAAGCGCCCCAGGTTTACAAAAAACTGATCCAGCGCGAAAAGGGACTGATCCTGGTAA
CCGGCCCGACAGGAAGCGGTAAGTCGACGACCCTCGCCGCCATGCTCAACGAAATCAACATGAGTGAACGCAAACATATC
ATCACGATCGAAGATCCCATCGAATTCGTTCACGAGAACCACCAGTCGCTCTTCTCCCACAGGAACATCGGAACCGACAC
CGACTCTTTCGCCACGGCGCTCAAATATGCCCTCCGTGAAGACCCCGACGTCATCCTGATCGGCGAGATGCGGGACAAAG
AGACGATCGCGGCGGCTCTGACGGCGGCGGAAACGGGCCACCTCGTCTTCGGTACGGTCCATACCAATTCGGCGGCACAG
ACCGTCAACCGTATCATCGACGTTTTCAGCGGGGACGAACAGCCCCAGATCCGGGCCCAGCTCTCCACCTCTCTCGTGGC
GGTCATTTCCCAGGCGCTCCTGCCCAAGATCGGGGGCGGACGTATCGCGGTTCCCGAAATTCTCATCAGCAACCCCGCCA
TCGCCAACCTGATCCGCGAAGATAAGGTGCACCAGATCTACAGCCAGATGCAGCTTGGCCAGGGCGAAAGCGGCATGCAG
ACCCAGACGCAGGTAATGGTCAAGCTTGTCAAAGGCCACATCATCGAAAAGAGCGAAGCGCTCCGCTTCTCGACCCGTCC
CGACGAACTCCTCAAAGCGCTCCAGCACGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

52.367

94.678

0.496
  pilT Neisseria meningitidis 8013

52.071

94.678

0.493
  pilT Pseudomonas stutzeri DSM 10701

50.435

96.639

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.096

93.557

0.487
  pilT Vibrio cholerae strain A1552

52.096

93.557

0.487
  pilT Acinetobacter baumannii strain A118

50.289

96.919

0.487
  pilT Acinetobacter nosocomialis M2

50.289

96.919

0.487
  pilT Acinetobacter baumannii D1279779

50.289

96.919

0.487
  pilT Pseudomonas aeruginosa PAK

51.642

93.838

0.485
  pilT Legionella pneumophila strain ERS1305867

51.343

93.838

0.482
  pilT Legionella pneumophila strain Lp02

51.343

93.838

0.482
  pilT Acinetobacter baylyi ADP1

49.275

96.639

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.272

97.759

0.443
  pilU Acinetobacter baylyi ADP1

42.651

97.199

0.415
  pilU Pseudomonas stutzeri DSM 10701

39.71

96.639

0.384
  pilU Vibrio cholerae strain A1552

37.322

98.319

0.367

Multiple sequence alignment