Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QCD67_RS19255 Genome accession   NZ_CP122401
Coordinates   3958677..3959657 (+) Length   326 a.a.
NCBI ID   WP_206805629.1    Uniprot ID   -
Organism   Enterobacter roggenkampii strain EOBSR_19     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3953677..3964657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QCD67_RS19215 (QCD67_19215) - 3953744..3954070 (+) 327 WP_003862421.1 YggL family protein -
  QCD67_RS19220 (QCD67_19220) - 3954127..3954843 (+) 717 WP_008499762.1 DUF2884 domain-containing protein -
  QCD67_RS19225 (QCD67_19225) - 3954919..3955281 (-) 363 WP_071949300.1 endonuclease domain-containing protein -
  QCD67_RS19230 (QCD67_19230) hemW 3955345..3956487 (-) 1143 WP_206805628.1 radical SAM family heme chaperone HemW -
  QCD67_RS19235 (QCD67_19235) - 3956480..3957073 (-) 594 WP_032653325.1 XTP/dITP diphosphatase -
  QCD67_RS19240 (QCD67_19240) yggU 3957077..3957373 (-) 297 WP_008499758.1 DUF167 family protein YggU -
  QCD67_RS19245 (QCD67_19245) - 3957370..3957936 (-) 567 WP_008499757.1 YggT family protein -
  QCD67_RS19250 (QCD67_19250) - 3957958..3958659 (-) 702 WP_008499756.1 YggS family pyridoxal phosphate-dependent enzyme -
  QCD67_RS19255 (QCD67_19255) pilT 3958677..3959657 (+) 981 WP_206805629.1 type IV pilus twitching motility protein PilT Machinery gene
  QCD67_RS19260 (QCD67_19260) ruvX 3959668..3960084 (-) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  QCD67_RS19265 (QCD67_19265) - 3960084..3960644 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  QCD67_RS19270 (QCD67_19270) gshB 3960727..3961674 (-) 948 WP_008499751.1 glutathione synthase -
  QCD67_RS19275 (QCD67_19275) rsmE 3961694..3962425 (-) 732 WP_055322222.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QCD67_RS19280 (QCD67_19280) endA 3962477..3963184 (-) 708 WP_206805630.1 deoxyribonuclease I -
  QCD67_RS19285 (QCD67_19285) - 3963279..3963776 (-) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35479.45 Da        Isoelectric Point: 6.6962

>NTDB_id=815427 QCD67_RS19255 WP_206805629.1 3958677..3959657(+) (pilT) [Enterobacter roggenkampii strain EOBSR_19]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKAWLNDEQQGIWWANGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRAIPELLTNASGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLVPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=815427 QCD67_RS19255 WP_206805629.1 3958677..3959657(+) (pilT) [Enterobacter roggenkampii strain EOBSR_19]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCCGACGTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAACGGGCAGGTGGATTTTGCCGCGACGGTGGCAGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGAGTCTCTGTGACGCTGCGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGCAATCCCGGAACTGTTAACCAATGCGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATTCTCACGCTCGAAGATCCGGTGGAG
TTTATCTATCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATCGGCCAGCACAGCCCGTCATTTGCCGATGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTGATTTTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCGGAGACCGGGCACCTGGTGCTGGCGACGCTGCACACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAGCTGGTTCC
TGACTTACAGGACGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACCGCGGCGGCGGCAAATCTGATACGAGAAGGAA
AAACGTGGCAGCTGCCCGGCATTATTCAAACCGGACAGCAGGCAGGCATGCAGAATTTTGACCAAAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365