Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P8596_RS16690 Genome accession   NZ_CP122397
Coordinates   3190794..3191837 (-) Length   347 a.a.
NCBI ID   WP_279724385.1    Uniprot ID   -
Organism   Rossellomorea sp. DA94     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3185794..3196837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8596_RS16660 (P8596_16660) - 3185850..3186635 (-) 786 WP_279724380.1 hypothetical protein -
  P8596_RS16665 (P8596_16665) - 3186632..3187249 (-) 618 WP_279724381.1 pilus assembly protein PilN -
  P8596_RS16670 (P8596_16670) pilM 3187249..3188211 (-) 963 WP_279724382.1 pilus assembly protein PilM -
  P8596_RS16675 (P8596_16675) - 3188227..3188970 (-) 744 WP_279727019.1 prepilin peptidase -
  P8596_RS16680 (P8596_16680) - 3189000..3189470 (-) 471 WP_279724383.1 type II secretion system protein -
  P8596_RS16685 (P8596_16685) - 3189582..3190793 (-) 1212 WP_279724384.1 type II secretion system F family protein -
  P8596_RS16690 (P8596_16690) pilT 3190794..3191837 (-) 1044 WP_279724385.1 type IV pilus twitching motility protein PilT Machinery gene
  P8596_RS16695 (P8596_16695) - 3191847..3193508 (-) 1662 WP_279724386.1 ATPase, T2SS/T4P/T4SS family -
  P8596_RS16700 (P8596_16700) - 3193530..3194915 (-) 1386 WP_279724387.1 G5 domain-containing protein -
  P8596_RS16705 (P8596_16705) - 3194943..3195677 (-) 735 WP_279724388.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38581.60 Da        Isoelectric Point: 7.6698

>NTDB_id=815358 P8596_RS16690 WP_279724385.1 3190794..3191837(-) (pilT) [Rossellomorea sp. DA94]
MKEKVDYLLKSAFELGASDIHLTVGSPPIFRIHGDLKRYGKENLLPDHTELMAKAIISETMYPAFEEVGELDFSYGIPGV
SRFRVNAFKQRSCVSLAIRVIPTSIPTMEDLFLPHALKAIAEKPQGLFLVTGPTGSGKSTTLASIVDYMNRKMRKHIITL
EDPIEYLHKHGASIIDQREVGFDTRSFSKGLRSALRQDPDIILVGEMRDLETIHTAITAAETGHLVLATLHTSSAPATIE
RIVDVFPPEQQSQIRVQLASVLVGILSQRLFATVDKKGRRAATELLMNNAAIANLIRSEKVHQIQNVMQTSKSSGMHIMS
DSVMRLYESGIISKEMAFPYSREEIPQ

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=815358 P8596_RS16690 WP_279724385.1 3190794..3191837(-) (pilT) [Rossellomorea sp. DA94]
TTGAAAGAAAAAGTTGATTATCTTCTTAAATCCGCTTTCGAGCTGGGGGCGTCGGATATCCATTTGACCGTGGGTTCACC
GCCTATATTCCGCATACACGGGGATTTAAAAAGATACGGGAAAGAGAACCTGTTGCCCGATCATACAGAGTTAATGGCAA
AAGCCATCATTTCAGAAACGATGTATCCTGCTTTTGAAGAAGTGGGTGAGCTGGATTTTTCCTATGGGATTCCCGGCGTG
TCAAGATTCAGGGTGAATGCATTTAAGCAGCGATCATGTGTATCCTTGGCTATACGTGTGATTCCAACTTCCATTCCGAC
AATGGAAGATCTGTTTCTTCCACATGCATTGAAGGCCATTGCCGAAAAACCTCAGGGACTGTTCCTTGTAACAGGGCCAA
CGGGAAGCGGGAAGTCTACCACACTCGCTTCCATCGTCGATTACATGAACCGGAAGATGAGGAAGCATATCATCACCCTG
GAAGACCCAATCGAGTACCTGCATAAACACGGTGCATCGATCATCGATCAGCGGGAAGTGGGATTTGATACAAGATCATT
CTCAAAAGGATTGAGAAGTGCCCTGAGGCAGGATCCGGATATCATTCTTGTAGGAGAGATGCGTGACCTGGAGACCATCC
ATACGGCCATCACGGCAGCTGAGACCGGTCACCTTGTACTTGCGACCCTTCACACATCCAGTGCGCCTGCCACCATCGAG
CGTATCGTAGACGTCTTCCCTCCTGAGCAACAGTCGCAAATCCGTGTTCAGTTGGCCTCGGTCCTTGTCGGGATCCTTTC
CCAAAGGCTATTTGCCACCGTGGATAAAAAAGGGAGAAGGGCCGCGACGGAGCTCCTGATGAATAATGCGGCGATCGCCA
ATCTGATCCGTTCTGAAAAGGTCCACCAGATTCAAAATGTCATGCAGACGTCTAAAAGTTCAGGCATGCACATCATGAGC
GACAGTGTGATGAGACTATATGAGAGTGGCATTATATCTAAGGAAATGGCGTTTCCTTACAGCAGAGAGGAGATTCCTCA
GTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.829

94.524

0.49
  pilT Pseudomonas aeruginosa PAK

48.105

98.847

0.476
  pilT Pseudomonas stutzeri DSM 10701

49.401

96.254

0.476
  pilT Legionella pneumophila strain Lp02

47.447

95.965

0.455
  pilT Legionella pneumophila strain ERS1305867

47.447

95.965

0.455
  pilT Acinetobacter nosocomialis M2

47.273

95.101

0.45
  pilT Acinetobacter baumannii D1279779

47.273

95.101

0.45
  pilT Acinetobacter baumannii strain A118

47.273

95.101

0.45
  pilT Acinetobacter baylyi ADP1

46.97

95.101

0.447
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.646

94.524

0.441
  pilT Vibrio cholerae strain A1552

46.646

94.524

0.441
  pilT Neisseria meningitidis 8013

46.417

92.507

0.429
  pilT Neisseria gonorrhoeae MS11

46.106

92.507

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.617

96.254

0.401
  pilU Vibrio cholerae strain A1552

40.994

92.795

0.38
  pilU Acinetobacter baylyi ADP1

39.571

93.948

0.372