Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RZV12_RS21040 Genome accession   NZ_CP136552
Coordinates   4267359..4268339 (-) Length   326 a.a.
NCBI ID   WP_001349546.1    Uniprot ID   B7LFK8
Organism   Escherichia coli O104:H4 strain rpoS_ATGtoATA     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4262359..4273339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV12_RS21010 yggI 4263022..4263519 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  RZV12_RS21015 endA 4263614..4264321 (+) 708 WP_000286500.1 deoxyribonuclease I -
  RZV12_RS21020 rsmE 4264401..4265132 (+) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RZV12_RS21025 gshB 4265145..4266095 (+) 951 WP_000593273.1 glutathione synthase -
  RZV12_RS21030 yqgE 4266204..4266767 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  RZV12_RS21035 ruvX 4266767..4267183 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  RZV12_RS21040 pilT 4267359..4268339 (-) 981 WP_001349546.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZV12_RS21045 yggS 4268357..4269061 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  RZV12_RS21050 yggT 4269079..4269645 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  RZV12_RS21055 yggU 4269642..4269932 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  RZV12_RS21060 rdgB 4269940..4270533 (+) 594 WP_001174743.1 XTP/dITP diphosphatase -
  RZV12_RS21065 hemW 4270526..4271662 (+) 1137 WP_000239939.1 radical SAM family heme chaperone HemW -
  RZV12_RS21070 yggM 4271817..4272824 (-) 1008 WP_000745217.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35942.12 Da        Isoelectric Point: 5.7980

>NTDB_id=815065 RZV12_RS21040 WP_001349546.1 4267359..4268339(-) (pilT) [Escherichia coli O104:H4 strain rpoS_ATGtoATA]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=815065 RZV12_RS21040 WP_001349546.1 4267359..4268339(-) (pilT) [Escherichia coli O104:H4 strain rpoS_ATGtoATA]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365