Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QBS15_RS17315 Genome accession   NZ_CP121842
Coordinates   3745840..3746874 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 16702     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3740840..3751874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS15_RS17285 glnK 3741420..3741758 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS15_RS17290 arfB 3741960..3742376 (+) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS15_RS17295 - 3742409..3742900 (+) 492 WP_201914207.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS15_RS17300 - 3743261..3743686 (-) 426 WP_005340360.1 hypothetical protein -
  QBS15_RS17305 yaaA 3743881..3744654 (-) 774 WP_279461045.1 peroxide stress protein YaaA -
  QBS15_RS17310 pilU 3744702..3745811 (-) 1110 WP_129504783.1 type IVa pilus ATPase TapU Machinery gene
  QBS15_RS17315 pilT 3745840..3746874 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS15_RS17320 - 3746914..3747615 (+) 702 WP_139441948.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS15_RS17325 proC 3747758..3748582 (+) 825 WP_139441947.1 pyrroline-5-carboxylate reductase -
  QBS15_RS17330 - 3748594..3749145 (+) 552 WP_047436998.1 YggT family protein -
  QBS15_RS17335 yggU 3749145..3749447 (+) 303 WP_005340341.1 DUF167 family protein YggU -
  QBS15_RS17340 - 3749491..3749910 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -
  QBS15_RS17345 - 3750012..3751208 (-) 1197 WP_279461050.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=814617 QBS15_RS17315 WP_005340354.1 3745840..3746874(-) (pilT) [Aeromonas veronii strain ANYA 16702]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=814617 QBS15_RS17315 WP_005340354.1 3745840..3746874(-) (pilT) [Aeromonas veronii strain ANYA 16702]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGATCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACGGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATTTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTACTGGTGACCGGCCCTACCGGCT
CGGGTAAATCGACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAGGAT
CCCATCGAGTTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTTCATCGGGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTTTGG
CCATGACGGCCGCCGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGTGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATTC
GGGAGGACAAGATCGCCCAGCTTTACTCGGTGATCCAGACCGGGATGACCCACGGTATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGTGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTCGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407