Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QA656_RS12650 Genome accession   NZ_CP121696
Coordinates   2790514..2791566 (-) Length   350 a.a.
NCBI ID   WP_096043513.1    Uniprot ID   -
Organism   Clostridium botulinum strain BT-22100019     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2785514..2796566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QA656_RS12630 (QA656_12605) ftsZ 2786018..2787127 (-) 1110 WP_003484662.1 cell division protein FtsZ -
  QA656_RS12635 (QA656_12610) ftsA 2787150..2788403 (-) 1254 WP_096043515.1 cell division protein FtsA -
  QA656_RS12640 (QA656_12615) - 2788552..2789826 (-) 1275 WP_096043514.1 glycosyltransferase family 2 protein -
  QA656_RS12645 (QA656_12620) - 2790269..2790403 (+) 135 WP_045896738.1 hypothetical protein -
  QA656_RS12650 (QA656_12625) pilT 2790514..2791566 (-) 1053 WP_096043513.1 type IV pilus twitching motility protein PilT Machinery gene
  QA656_RS12655 (QA656_12630) aroE 2791568..2792344 (-) 777 WP_096043512.1 shikimate dehydrogenase -
  QA656_RS12660 (QA656_12635) sigK 2792482..2793186 (-) 705 WP_003484649.1 RNA polymerase sporulation sigma factor SigK -
  QA656_RS12665 (QA656_12640) - 2793283..2794953 (-) 1671 WP_096043511.1 penicillin-binding protein 2 -
  QA656_RS12670 (QA656_12645) udk 2795039..2795659 (-) 621 WP_096043510.1 uridine kinase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39053.12 Da        Isoelectric Point: 8.6835

>NTDB_id=813984 QA656_RS12650 WP_096043513.1 2790514..2791566(-) (pilT) [Clostridium botulinum strain BT-22100019]
MKQLKELLEKTINFGASDLHLSVGIEPTIRINGQLSKIFKEKLTPENTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREVISYDTALNYSVDKDLITKIINL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=813984 QA656_RS12650 WP_096043513.1 2790514..2791566(-) (pilT) [Clostridium botulinum strain BT-22100019]
TTGAAACAATTAAAAGAATTATTGGAAAAAACTATTAACTTTGGCGCTTCAGATTTACATTTATCCGTAGGGATAGAACC
AACTATAAGAATAAATGGTCAATTAAGCAAAATATTCAAAGAAAAGCTCACACCAGAAAATACTAATAATTATGCTAAAG
AAATATTAGGTGAAGAATTTGAGGATTTATTAAAAAAAGGAGAAATAGATACCTCCTATTCTATATCAGGCTTAGGTAGA
TTTAGAGTAAGTGTGTTTAAACAAAGGGGAAGTATAGCTATTTCTTTAAGGGTTGTAAGTTTAAAGATTCCTAACTTAAA
TGAATTAAAATTACCTGATATAGTAAAGGAATTAACAAATAAAAAAAGAGGCCTTATTTTAGTAACAGGTCCTACAGGTT
CAGGTAAAAGCACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACCTTAGAAGAT
CCAATAGAATTTTTACATAAACATAACAAATGTATAATAAATCAAAGAGAGATAGGTAGGGATACTTTTAGTTATAAAAG
TGCTTTAAAAGCAGCATTAAGAGAAGATCCGGATGTAATATTAATAGGAGAGCTTAGAGACTTAGAAGATATTTCTATAG
CTTTAACAGCTGCAGAAACAGGGCATTTAGTATTTTCTACTTTGCATACTATAGGAGGGGCTAAAACTATAGATAGAATT
GTTAATGTATTTCCGCCACATCAACAGGAACAGATAAGAATACAAGTAGCAAGTGTGTTAGAAGGAATAATAAGTCAACA
ATTAGTACATAAAATTGATGGAGGAAGAATAGCAGCCTTAGAGACTATGGTAACTACAAATGCCATAAGAAATATGATAA
GGGAAGGAAAAACTTATCAAATAGAATCTTCCATACAAACTGGATCCAAATATGGAATGAAAACTATGGATATGTCTTTA
GCAGAGTTATATAAAAGAGAAGTTATAAGTTATGATACTGCATTAAATTATTCAGTAGACAAAGATTTAATTACAAAGAT
AATTAATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.751

95.143

0.483
  pilT Pseudomonas aeruginosa PAK

49.405

96

0.474
  pilT Legionella pneumophila strain Lp02

48.116

98.571

0.474
  pilT Legionella pneumophila strain ERS1305867

48.116

98.571

0.474
  pilT Acinetobacter baumannii D1279779

49.55

95.143

0.471
  pilT Acinetobacter nosocomialis M2

49.55

95.143

0.471
  pilT Acinetobacter baumannii strain A118

49.55

95.143

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.81

96

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

94.286

0.469
  pilT Vibrio cholerae strain A1552

49.697

94.286

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.977

99.429

0.457
  pilT Neisseria gonorrhoeae MS11

47.761

95.714

0.457
  pilT Neisseria meningitidis 8013

47.761

95.714

0.457
  pilU Acinetobacter baylyi ADP1

40.828

96.571

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.896

95.714

0.391
  pilU Vibrio cholerae strain A1552

38.348

96.857

0.371