Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RS168_RS09295 Genome accession   NZ_CP135658
Coordinates   1854443..1855423 (-) Length   326 a.a.
NCBI ID   WP_001604836.1    Uniprot ID   -
Organism   Escherichia coli strain TUM10008     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1849443..1860423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS168_RS09265 (RS168_09265) yggI 1850167..1850664 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  RS168_RS09270 (RS168_09270) endA 1850759..1851466 (+) 708 WP_000286492.1 deoxyribonuclease I -
  RS168_RS09275 (RS168_09275) rsmE 1851546..1852277 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RS168_RS09280 (RS168_09280) gshB 1852290..1853240 (+) 951 WP_000593260.1 glutathione synthase -
  RS168_RS09285 (RS168_09285) yqgE 1853349..1853912 (+) 564 WP_001301052.1 YqgE/AlgH family protein -
  RS168_RS09290 (RS168_09290) ruvX 1853912..1854328 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  RS168_RS09295 (RS168_09295) pilT 1854443..1855423 (-) 981 WP_001604836.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RS168_RS09300 (RS168_09300) yggS 1855441..1856145 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  RS168_RS09305 (RS168_09305) yggT 1856163..1856729 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  RS168_RS09310 (RS168_09310) yggU 1856726..1857016 (+) 291 WP_097680476.1 DUF167 family protein YggU -
  RS168_RS09315 (RS168_09315) rdgB 1857024..1857617 (+) 594 WP_001174747.1 XTP/dITP diphosphatase -
  RS168_RS09320 (RS168_09320) hemW 1857610..1858746 (+) 1137 WP_115800943.1 radical SAM family heme chaperone HemW -
  RS168_RS09325 (RS168_09325) - 1859083..1859779 (+) 697 Protein_1832 IS1-like element IS1A family transposase -
  RS168_RS09330 (RS168_09330) - 1859796..1860401 (-) 606 Protein_1833 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36033.27 Da        Isoelectric Point: 5.7980

>NTDB_id=811289 RS168_RS09295 WP_001604836.1 1854443..1855423(-) (pilT) [Escherichia coli strain TUM10008]
MYMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINIPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=811289 RS168_RS09295 WP_001604836.1 1854443..1855423(-) (pilT) [Escherichia coli strain TUM10008]
ATGTATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCATTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTGGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACATTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTAGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.405

96.933

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

38.125

98.16

0.374
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362