Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P8F83_RS05035 Genome accession   NZ_CP121166
Coordinates   978883..979938 (+) Length   351 a.a.
NCBI ID   WP_315109926.1    Uniprot ID   -
Organism   Clostridium intestinale strain PC17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 973883..984938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8F83_RS05015 (P8F83_05020) - 974453..975676 (+) 1224 WP_326513708.1 U32 family peptidase -
  P8F83_RS05020 (P8F83_05025) udk 975688..976308 (+) 621 WP_073015857.1 uridine kinase -
  P8F83_RS05025 (P8F83_05030) - 976360..977964 (+) 1605 WP_326513709.1 penicillin-binding transpeptidase domain-containing protein -
  P8F83_RS05030 (P8F83_05035) sigK 978042..978740 (+) 699 WP_275299588.1 RNA polymerase sporulation sigma factor SigK -
  P8F83_RS05035 (P8F83_05040) pilT 978883..979938 (+) 1056 WP_315109926.1 type IV pilus twitching motility protein PilT Machinery gene
  P8F83_RS05040 (P8F83_05045) - 980114..981388 (+) 1275 WP_326513710.1 glycosyltransferase family 2 protein -
  P8F83_RS05045 (P8F83_05050) - 981723..981908 (+) 186 WP_326513711.1 hypothetical protein -
  P8F83_RS05050 (P8F83_05055) ftsZ 981967..983064 (+) 1098 WP_238904672.1 cell division protein FtsZ -
  P8F83_RS05055 (P8F83_05060) - 983235..984038 (+) 804 WP_326513712.1 sigma-E processing peptidase SpoIIGA -
  P8F83_RS05060 (P8F83_05065) sigE 984054..984761 (+) 708 WP_021802195.1 RNA polymerase sporulation sigma factor SigE -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38831.93 Da        Isoelectric Point: 7.0351

>NTDB_id=810769 P8F83_RS05035 WP_315109926.1 978883..979938(+) (pilT) [Clostridium intestinale strain PC17]
MYVLVELLSKTLELGASDLHLTVGIPPVIRVNGGLIKIGSSNLTPVDTEKFAKEILEEKFTEYSQVGEYDTSYSIAGLGR
FRVNVFKQRNSDALALRVIALKIPTLQELSHPPVLKELTEKKRGLVLVTGPTGSGKSTTLAAMLNEINHNREAHVITLED
PIEFLHKHDKCIINQREIGKDSNSYQNALRAILREDPDVILVGEMRDLETIAIAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQQQIRIQLSAVLQGIVSQQLVPTADKQSRVAALEVMITTPGIQNIIREGKTHQIESSIQTGSKYGMKTMDMA
LAELYRKGIITQETALSYAVDRETIKRIMLL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=810769 P8F83_RS05035 WP_315109926.1 978883..979938(+) (pilT) [Clostridium intestinale strain PC17]
ATGTACGTTTTAGTTGAACTGTTAAGTAAAACATTAGAGTTAGGGGCATCAGATTTGCATCTTACAGTAGGAATTCCACC
TGTAATTAGAGTTAATGGTGGATTGATTAAAATAGGAAGTTCTAATCTAACTCCTGTAGACACCGAAAAATTTGCAAAAG
AAATACTAGAAGAAAAATTCACAGAATATAGTCAAGTGGGTGAATATGATACATCTTATTCAATTGCTGGATTAGGTAGA
TTTAGAGTGAATGTATTTAAACAAAGAAATTCAGATGCATTAGCACTTAGAGTTATTGCATTAAAGATACCTACACTTCA
AGAGCTTAGTCATCCACCTGTATTAAAAGAGTTAACTGAGAAAAAGAGAGGATTAGTTCTAGTTACTGGGCCAACTGGAA
GTGGTAAATCTACAACTTTGGCTGCCATGCTTAATGAGATAAATCATAATAGAGAAGCTCATGTAATAACTTTAGAAGAT
CCTATAGAATTTTTACATAAACATGATAAGTGTATAATTAATCAAAGAGAAATAGGAAAAGATTCAAATAGTTATCAAAA
TGCTTTAAGAGCTATATTAAGAGAGGACCCTGATGTTATTCTAGTTGGAGAGATGAGAGATCTTGAAACTATAGCTATAG
CAATAACAGCTGCTGAAACAGGGCATTTAGTCTTTTCAACATTACATACTATTGGAGCTGCAAAGACGATAGATAGAATA
GTAGATGTTTTCCCTCCGCACCAACAACAACAAATAAGAATTCAATTGTCAGCAGTGTTACAAGGAATTGTTTCGCAGCA
ATTGGTACCAACAGCTGATAAACAAAGTAGAGTAGCAGCATTAGAAGTTATGATCACTACCCCAGGAATTCAAAATATAA
TAAGAGAAGGAAAAACCCATCAAATTGAATCATCAATACAAACTGGAAGCAAATATGGAATGAAGACCATGGATATGGCT
TTAGCTGAGTTATATAGAAAAGGAATTATTACTCAAGAGACAGCTTTAAGTTATGCCGTTGATAGAGAGACTATTAAAAG
AATTATGTTGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

51.176

96.866

0.496
  pilT Legionella pneumophila strain ERS1305867

51.176

96.866

0.496
  pilT Acinetobacter baylyi ADP1

50.437

97.721

0.493
  pilT Pseudomonas aeruginosa PAK

50.595

95.726

0.484
  pilT Acinetobacter nosocomialis M2

48.98

97.721

0.479
  pilT Acinetobacter baumannii D1279779

48.98

97.721

0.479
  pilT Acinetobacter baumannii strain A118

48.98

97.721

0.479
  pilT Pseudomonas stutzeri DSM 10701

49.55

94.872

0.47
  pilT Neisseria meningitidis 8013

48.105

97.721

0.47
  pilT Neisseria gonorrhoeae MS11

47.813

97.721

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.11

98.575

0.464
  pilT Vibrio cholerae strain A1552

48.78

93.447

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.78

93.447

0.456
  pilU Pseudomonas stutzeri DSM 10701

43.931

98.575

0.433
  pilU Vibrio cholerae strain A1552

40.23

99.145

0.399
  pilU Acinetobacter baylyi ADP1

40.233

97.721

0.393