Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JG736_RS03995 Genome accession   NZ_AP023059
Coordinates   728885..729946 (-) Length   353 a.a.
NCBI ID   WP_201338477.1    Uniprot ID   -
Organism   Nitratiruptor sp. YY08-13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 723885..734946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG736_RS03975 (NitYY0813_C0795) - 724642..725412 (-) 771 WP_201338473.1 SPOR domain-containing protein -
  JG736_RS03980 (NitYY0813_C0796) - 725488..726735 (-) 1248 WP_201338474.1 serine hydroxymethyltransferase -
  JG736_RS03985 (NitYY0813_C0797) lysS 726735..728246 (-) 1512 WP_370583674.1 lysine--tRNA ligase -
  JG736_RS03990 (NitYY0813_C0798) - 728246..728875 (-) 630 WP_201338476.1 CvpA family protein -
  JG736_RS03995 (NitYY0813_C0799) pilT 728885..729946 (-) 1062 WP_201338477.1 type IV pilus twitching motility protein PilT Machinery gene
  JG736_RS04000 (NitYY0813_C0800) gatC 729954..730247 (-) 294 WP_201338478.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  JG736_RS04005 (NitYY0813_C0801) - 730334..730939 (+) 606 WP_201338479.1 tetratricopeptide repeat protein -
  JG736_RS04010 (NitYY0813_C0802) - 730936..731937 (+) 1002 WP_201338480.1 hypothetical protein -
  JG736_RS04015 (NitYY0813_C0803) - 731934..732257 (+) 324 WP_201338481.1 hypothetical protein -
  JG736_RS04020 (NitYY0813_C0804) - 732244..732858 (+) 615 WP_201338482.1 type III pantothenate kinase -
  JG736_RS04025 (NitYY0813_C0805) hisG 732852..733469 (+) 618 WP_201338483.1 ATP phosphoribosyltransferase -
  JG736_RS04030 (NitYY0813_C0806) - 733459..734136 (+) 678 WP_201338484.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39541.79 Da        Isoelectric Point: 8.1113

>NTDB_id=80875 JG736_RS03995 WP_201338477.1 728885..729946(-) (pilT) [Nitratiruptor sp. YY08-13]
MVDINSLLKTVVAHKASDLHLVFGSEPQVRIDGRLIPLNLPVLTGKEIQTLCYSLLTDKQKKEFEENYELDFAFSIPNVG
RFRANYYKTLGEVAAAFRIIPQEIPSIDDLGQPPIFKDIIKREKGLILVTGPTGSGKSTTLAAMLNEINNVEHKHIITIE
DPVEFIHKNKKSLFSHRELGFDTKSFAVALKYALREDPDVILIGEMRDRETIKAALTAAETGHLVFGTLHTNSAPQTINR
IIDVFGGDEQPQIRAQLSTSLVAVISQALLPKIEGGRVAVYEILVNNPAIANLIRENKIHQIYSQMQLGRQQTGMQTQTK
VLQKLLLQKVIDKETAIKFSNRPEELMNFIKNL

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=80875 JG736_RS03995 WP_201338477.1 728885..729946(-) (pilT) [Nitratiruptor sp. YY08-13]
ATGGTAGATATCAACTCTTTACTCAAAACTGTTGTGGCACATAAAGCTTCAGATCTCCATTTGGTTTTTGGGAGTGAACC
GCAAGTAAGAATCGATGGAAGACTTATCCCACTCAATCTTCCTGTCTTAACTGGCAAAGAGATACAAACATTGTGCTACT
CTCTCTTAACTGATAAACAAAAGAAAGAGTTTGAAGAAAATTATGAGCTTGATTTTGCCTTCAGTATCCCCAATGTTGGA
CGTTTCCGTGCAAACTATTACAAAACTTTAGGAGAAGTTGCAGCTGCATTTCGTATTATTCCACAAGAAATTCCAAGTAT
TGATGATTTGGGACAGCCTCCTATATTTAAAGATATTATTAAACGAGAAAAGGGACTCATACTTGTCACTGGTCCTACTG
GAAGTGGTAAATCAACTACTCTCGCTGCAATGCTTAATGAAATTAACAATGTCGAGCATAAGCATATAATTACTATAGAA
GATCCTGTTGAGTTTATACATAAAAATAAAAAATCCCTCTTTTCCCACCGCGAGTTAGGATTTGATACAAAAAGTTTTGC
AGTCGCGCTCAAATATGCCTTGCGTGAAGATCCCGATGTCATATTGATTGGAGAGATGCGAGATCGTGAAACAATCAAAG
CAGCCCTCACTGCAGCTGAAACTGGTCACTTGGTATTTGGGACCCTCCATACCAACTCTGCACCTCAAACTATCAATAGA
ATTATAGATGTTTTTGGTGGTGATGAGCAACCACAAATAAGAGCACAACTCTCCACAAGTCTTGTGGCTGTTATATCCCA
AGCACTTTTGCCAAAAATTGAAGGTGGAAGAGTAGCTGTTTATGAAATCCTCGTCAACAATCCAGCTATCGCAAACCTTA
TTCGAGAAAACAAAATACACCAGATCTATTCACAAATGCAGTTAGGGCGGCAACAAACAGGTATGCAAACACAAACAAAA
GTTTTACAAAAACTCCTCTTACAAAAAGTAATAGACAAAGAGACTGCAATAAAATTCTCTAATAGACCAGAAGAGTTAAT
GAACTTTATAAAAAATCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

54.627

94.901

0.518
  pilT Legionella pneumophila strain ERS1305867

54.627

94.901

0.518
  pilT Acinetobacter baumannii D1279779

52.89

98.017

0.518
  pilT Neisseria gonorrhoeae MS11

52.89

98.017

0.518
  pilT Acinetobacter baumannii strain A118

52.89

98.017

0.518
  pilT Acinetobacter nosocomialis M2

52.89

98.017

0.518
  pilT Neisseria meningitidis 8013

52.601

98.017

0.516
  pilT Vibrio cholerae strain A1552

54.354

94.334

0.513
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.354

94.334

0.513
  pilT Acinetobacter baylyi ADP1

52.174

97.734

0.51
  pilT Pseudomonas stutzeri DSM 10701

51.895

97.167

0.504
  pilT Pseudomonas aeruginosa PAK

51.603

97.167

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.266

98.017

0.473
  pilU Acinetobacter baylyi ADP1

43.228

98.3

0.425
  pilU Pseudomonas stutzeri DSM 10701

39.601

99.433

0.394
  pilU Vibrio cholerae strain A1552

37.892

99.433

0.377

Multiple sequence alignment