Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P4S50_RS02890 Genome accession   NZ_CP120733
Coordinates   677122..678171 (+) Length   349 a.a.
NCBI ID   WP_277733002.1    Uniprot ID   -
Organism   Tepidibacter hydrothermalis strain SWIR-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 672122..683171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4S50_RS02875 (P4S50_02875) - 672452..674476 (+) 2025 WP_277732999.1 methyl-accepting chemotaxis protein -
  P4S50_RS02880 (P4S50_02880) - 674727..675410 (+) 684 WP_277733000.1 DUF881 domain-containing protein -
  P4S50_RS02885 (P4S50_02885) - 675430..677112 (+) 1683 WP_277733001.1 GspE/PulE family protein -
  P4S50_RS02890 (P4S50_02890) pilT 677122..678171 (+) 1050 WP_277733002.1 type IV pilus twitching motility protein PilT Machinery gene
  P4S50_RS02895 (P4S50_02895) - 678183..679394 (+) 1212 WP_277733003.1 type II secretion system F family protein -
  P4S50_RS02900 (P4S50_02900) - 679691..680080 (+) 390 WP_277733004.1 competence type IV pilus major pilin ComGC -
  P4S50_RS02905 (P4S50_02905) - 680149..680895 (+) 747 WP_277733005.1 prepilin peptidase -
  P4S50_RS02910 (P4S50_02910) pilM 680914..681972 (+) 1059 WP_277733006.1 type IV pilus assembly protein PilM -
  P4S50_RS02915 (P4S50_02915) - 681983..682543 (+) 561 WP_277733007.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38828.83 Da        Isoelectric Point: 6.7783

>NTDB_id=808468 P4S50_RS02890 WP_277733002.1 677122..678171(+) (pilT) [Tepidibacter hydrothermalis strain SWIR-1]
MNIYDLLEKTIELGASDLHITVGFPPVMRINGKLSKYGEDILLPDDNLSLVKQILDEEKFEKLEEKGELDTSVSYHKLGR
FRVNIFKQRGTYCMAIRSVALEIPSMEKLGLPPVIKELAKKQRGLVLVTGPTGSGKSTTLASVIDYINKNRSEHILTLED
PIEYLHKHNKSIVNQREIGTDSKSFANALRSSLRQDPDVILVGEMRDLETISIALTAAETGHLVFSTLHTIGAAKTIDRI
IDVFPSHQQDQILVQLSAVLEGVISQQLISKEDGSGRVAALEVMVATTAIRNLIREGKTHQIQTSIQTGMKFGMQTMDNE
LRNLYAKGIISKEALLTYAVDKDMVSRYI

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=808468 P4S50_RS02890 WP_277733002.1 677122..678171(+) (pilT) [Tepidibacter hydrothermalis strain SWIR-1]
ATGAACATATATGATTTACTTGAAAAAACAATAGAATTAGGGGCATCAGATCTTCATATAACAGTTGGATTTCCACCTGT
TATGAGGATAAATGGAAAGCTTAGTAAGTATGGGGAAGATATATTATTGCCAGATGATAATTTGAGTTTGGTAAAGCAAA
TACTAGATGAAGAAAAATTTGAAAAATTAGAAGAAAAAGGTGAATTGGATACATCAGTTTCTTATCATAAATTAGGAAGA
TTTAGAGTAAATATATTTAAACAAAGAGGAACATATTGTATGGCTATAAGATCTGTAGCTTTAGAAATACCAAGTATGGA
AAAACTAGGTCTTCCACCTGTTATAAAGGAATTAGCTAAAAAACAAAGAGGCCTTGTACTTGTAACTGGACCTACAGGAA
GCGGAAAGTCAACGACATTGGCATCCGTTATTGATTACATAAATAAAAATAGAAGTGAGCACATATTAACATTAGAAGAT
CCTATAGAATATCTTCATAAACACAATAAAAGTATAGTTAATCAAAGAGAAATAGGTACTGACTCTAAGTCATTTGCAAA
TGCTTTAAGATCATCTTTAAGACAGGATCCTGATGTAATATTAGTTGGAGAGATGAGAGATTTAGAAACAATATCAATTG
CTCTAACAGCAGCAGAAACAGGACACTTAGTATTCTCAACACTTCATACTATAGGTGCTGCTAAAACTATAGATAGAATA
ATAGATGTTTTTCCATCTCACCAGCAAGATCAAATATTAGTACAGTTATCTGCAGTTCTTGAAGGAGTTATATCACAGCA
GTTGATTTCAAAAGAAGATGGAAGTGGAAGAGTTGCTGCACTTGAGGTAATGGTAGCAACTACAGCAATAAGAAATCTTA
TAAGAGAAGGAAAAACACATCAAATACAAACTTCTATACAAACGGGGATGAAGTTTGGAATGCAGACTATGGATAATGAA
CTAAGGAACTTATATGCAAAAGGAATTATATCAAAAGAAGCATTGTTGACTTATGCAGTAGATAAGGATATGGTCAGTAG
ATATATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

53.488

98.567

0.527
  pilT Legionella pneumophila strain ERS1305867

53.488

98.567

0.527
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.537

95.989

0.504
  pilT Vibrio cholerae strain A1552

52.537

95.989

0.504
  pilT Acinetobacter baumannii D1279779

51.327

97.135

0.499
  pilT Acinetobacter baumannii strain A118

51.327

97.135

0.499
  pilT Acinetobacter nosocomialis M2

51.032

97.135

0.496
  pilT Acinetobacter baylyi ADP1

51.964

94.842

0.493
  pilT Pseudomonas aeruginosa PAK

51.351

95.415

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.991

99.427

0.487
  pilT Pseudomonas stutzeri DSM 10701

50.299

95.702

0.481
  pilT Neisseria meningitidis 8013

47.11

99.14

0.467
  pilT Neisseria gonorrhoeae MS11

47.11

99.14

0.467
  pilU Pseudomonas stutzeri DSM 10701

43.658

97.135

0.424
  pilU Acinetobacter baylyi ADP1

42.353

97.421

0.413
  pilU Vibrio cholerae strain A1552

41.888

97.135

0.407