Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P5663_RS05870 Genome accession   NZ_CP120590
Coordinates   1137913..1138953 (+) Length   346 a.a.
NCBI ID   WP_144594034.1    Uniprot ID   -
Organism   Priestia flexa strain PRO116     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1132913..1143953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5663_RS05855 (P5663_05855) - 1133981..1134766 (+) 786 WP_277717297.1 PRC-barrel domain-containing protein -
  P5663_RS05860 (P5663_05860) - 1134783..1136222 (+) 1440 WP_277717298.1 VanW family protein -
  P5663_RS05865 (P5663_05865) - 1136235..1137901 (+) 1667 Protein_1071 GspE/PulE family protein -
  P5663_RS05870 (P5663_05870) pilT 1137913..1138953 (+) 1041 WP_144594034.1 type IV pilus twitching motility protein PilT Machinery gene
  P5663_RS05875 (P5663_05875) - 1138953..1140167 (+) 1215 WP_277717299.1 type II secretion system F family protein -
  P5663_RS05880 (P5663_05880) - 1140504..1140959 (+) 456 WP_277717300.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P5663_RS05885 (P5663_05885) - 1140968..1141723 (+) 756 WP_082802707.1 prepilin peptidase -
  P5663_RS05890 (P5663_05890) pilM 1141737..1142729 (+) 993 WP_025908541.1 type IV pilus biogenesis protein PilM -
  P5663_RS05895 (P5663_05895) - 1142726..1143352 (+) 627 WP_138116582.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38216.97 Da        Isoelectric Point: 7.6293

>NTDB_id=806368 P5663_RS05870 WP_144594034.1 1137913..1138953(+) (pilT) [Priestia flexa strain PRO116]
MRSKVEHLMRSAFQLKASDVHLTAGVPPIFRINGALKRYGKENLVPEELEEMAKNIIDPIRWGDFKEKGELDFSYAVEGL
SRFRVNAYHQRGSISIAIRIVPTKIPSLEDLKLPEVLKTIATKSQGLVLVTGPTGSGKSTTLASMIDFMNRTASRHIITL
EDPIEYLHRHGSCIIDQREVGFDTHSFANGLRASLRQDPDVILVGELRDLETIHTAITAAETGHLVLATLHTSSAPATIN
RIIDVFPAAQQEQIRVQLSSVLVSVISQRLLPTTDLQNRTCATEILMNNPAVSNLIRSEKIHQIPNVMQTSRSLGMHTLA
QSVNELLQAGVITKKVAEPYVNEAVS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=806368 P5663_RS05870 WP_144594034.1 1137913..1138953(+) (pilT) [Priestia flexa strain PRO116]
ATGAGAAGCAAAGTGGAACATTTAATGAGAAGTGCATTCCAGCTAAAGGCGTCAGACGTACATTTAACTGCAGGCGTCCC
GCCGATTTTTCGCATCAACGGTGCTTTAAAGCGCTATGGAAAAGAAAATTTAGTTCCAGAAGAGCTTGAAGAGATGGCAA
AGAATATTATTGATCCAATAAGATGGGGAGACTTCAAAGAAAAAGGCGAGCTCGATTTTTCATACGCTGTGGAAGGATTA
TCGCGCTTTCGCGTCAACGCTTATCATCAGCGCGGTTCAATTTCAATTGCCATTCGAATTGTGCCTACGAAGATTCCGAG
TTTAGAAGATTTAAAGCTTCCGGAAGTATTAAAAACAATTGCGACGAAATCTCAGGGACTTGTTCTTGTGACTGGACCAA
CGGGAAGTGGGAAGTCAACAACGCTTGCGTCTATGATCGATTTTATGAATCGTACGGCAAGTCGTCATATTATTACGCTA
GAAGATCCGATTGAGTACTTACATCGCCACGGTAGCTGCATTATTGATCAGCGGGAAGTGGGGTTTGATACACACAGCTT
TGCCAACGGTCTGCGCGCTTCGCTTCGTCAGGATCCTGACGTTATTCTAGTGGGAGAGCTTCGCGACCTAGAAACGATTC
ATACTGCCATTACGGCAGCTGAAACCGGACACTTAGTGCTCGCTACGCTGCATACGTCAAGCGCACCGGCAACGATTAAC
CGTATTATTGACGTATTCCCAGCTGCGCAGCAGGAGCAAATCCGCGTCCAGCTCTCTTCGGTGCTTGTGAGCGTCATTTC
ACAGCGTTTGCTGCCAACGACGGACTTGCAAAATCGCACGTGCGCAACGGAAATTTTAATGAATAATCCAGCTGTATCCA
ACCTGATTCGCAGTGAAAAAATTCATCAAATTCCAAACGTTATGCAAACAAGTCGTTCTCTTGGCATGCATACACTCGCT
CAGTCGGTCAATGAGCTTTTGCAAGCGGGTGTGATTACGAAAAAAGTAGCGGAACCGTATGTCAACGAGGCGGTTAGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.672

95.087

0.491
  pilT Legionella pneumophila strain ERS1305867

47.536

99.711

0.474
  pilT Legionella pneumophila strain Lp02

47.536

99.711

0.474
  pilT Acinetobacter nosocomialis M2

48.171

94.798

0.457
  pilT Acinetobacter baumannii D1279779

48.171

94.798

0.457
  pilT Acinetobacter baumannii strain A118

48.171

94.798

0.457
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.171

94.798

0.457
  pilT Vibrio cholerae strain A1552

48.171

94.798

0.457
  pilT Neisseria meningitidis 8013

48.916

93.353

0.457
  pilT Pseudomonas aeruginosa PAK

47.561

94.798

0.451
  pilT Pseudomonas stutzeri DSM 10701

47.416

95.087

0.451
  pilT Acinetobacter baylyi ADP1

47.256

94.798

0.448
  pilT Neisseria gonorrhoeae MS11

47.826

93.064

0.445
  pilU Pseudomonas stutzeri DSM 10701

43.413

96.532

0.419
  pilU Acinetobacter baylyi ADP1

45.833

90.173

0.413
  pilU Vibrio cholerae strain A1552

42.724

93.353

0.399