Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RKQ62_RS13500 Genome accession   NZ_CP134566
Coordinates   2781679..2782716 (+) Length   345 a.a.
NCBI ID   WP_391485223.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain XH1038     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2776679..2787716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKQ62_RS13490 (RKQ62_13535) - 2777062..2780658 (+) 3597 WP_002058592.1 AAA family ATPase -
  RKQ62_RS13495 (RKQ62_13540) - 2780860..2781552 (-) 693 WP_001108520.1 YggS family pyridoxal phosphate-dependent enzyme -
  RKQ62_RS13500 (RKQ62_13545) pilT 2781679..2782716 (+) 1038 WP_391485223.1 type IV pilus twitching motility protein PilT Machinery gene
  RKQ62_RS13505 (RKQ62_13550) pilU 2782744..2783862 (+) 1119 WP_000347036.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RKQ62_RS13510 (RKQ62_13555) fur 2783932..2784369 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  RKQ62_RS13515 (RKQ62_13560) - 2784481..2784879 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  RKQ62_RS13520 (RKQ62_13565) - 2784934..2785257 (-) 324 WP_000442589.1 RnfH family protein -
  RKQ62_RS13525 (RKQ62_13570) - 2785257..2786324 (-) 1068 WP_004835750.1 hypothetical protein -
  RKQ62_RS13530 (RKQ62_13575) - 2786477..2786932 (-) 456 WP_017897575.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38284.86 Da        Isoelectric Point: 6.5776

>NTDB_id=805588 RKQ62_RS13500 WP_391485223.1 2781679..2782716(+) (pilT) [Acinetobacter baumannii strain XH1038]
MDITELLAFSVKNGASDLQLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIHENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=805588 RKQ62_RS13500 WP_391485223.1 2781679..2782716(+) (pilT) [Acinetobacter baumannii strain XH1038]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACAACTTTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACATCAATCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTAG
CATTAACTGCTGCCGAAACAGGTCACTTGGTGTTTGGTACGCTCCACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGCGGTGGCCGTGTCGCAGCACATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
ATGAAAACAAAGTCGCGCAAATGTACTCAGCCATTCAAACTGGTGCCAATCATGGCATGACCACACTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTAATTAGCCCGCAAACGGCTCGTACTGCAGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

99.42

100

0.994
  pilT Acinetobacter baumannii D1279779

99.42

100

0.994
  pilT Acinetobacter nosocomialis M2

99.13

100

0.991
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.131

97.391

0.693
  pilT Vibrio cholerae strain A1552

71.131

97.391

0.693
  pilT Neisseria meningitidis 8013

67.536

100

0.675
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.101

0.391
  pilU Acinetobacter baylyi ADP1

38.439

100

0.386