Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PVE91_RS02315 Genome accession   NZ_CP120400
Coordinates   506476..507603 (-) Length   375 a.a.
NCBI ID   WP_053516991.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain LH-B2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501476..512603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE91_RS02295 - 501879..502868 (+) 990 WP_065175362.1 fumarylacetoacetate hydrolase family protein -
  PVE91_RS02300 maiA 502883..503551 (+) 669 WP_065175361.1 maleylacetoacetate isomerase -
  PVE91_RS02305 - 503584..505080 (-) 1497 WP_065175360.1 UdgX family uracil-DNA binding protein -
  PVE91_RS02310 - 505061..506344 (-) 1284 WP_065175359.1 putative DNA modification/repair radical SAM protein -
  PVE91_RS02315 pilU 506476..507603 (-) 1128 WP_053516991.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVE91_RS02320 - 507725..508090 (+) 366 WP_053516990.1 DUF4398 domain-containing protein -
  PVE91_RS02325 - 508087..508971 (+) 885 WP_277381372.1 hypothetical protein -
  PVE91_RS02330 - 509296..509532 (+) 237 WP_053516987.1 YdcH family protein -
  PVE91_RS02335 - 509837..511207 (+) 1371 WP_053516986.1 pyridoxal-phosphate dependent enzyme -
  PVE91_RS02340 - 511331..512503 (+) 1173 WP_253120224.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41445.71 Da        Isoelectric Point: 6.3738

>NTDB_id=805493 PVE91_RS02315 WP_053516991.1 506476..507603(-) (pilU) [Stenotrophomonas maltophilia strain LH-B2]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKDGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKSGVIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADYSAGQPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=805493 PVE91_RS02315 WP_053516991.1 506476..507603(-) (pilU) [Stenotrophomonas maltophilia strain LH-B2]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACCACCGGAGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGCCCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGGCCGATGCCGGCCGT
TTCCGGGTGAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAATCGACCTCGCTGGCGTCGATGATCGACCATCGCAACAGCACCACGACCGGACACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGATGTCCATCGTCAACCAGCGTGAAGTGGGGCTGGACACCCATGCCTTCCACAA
TGCGCTGAAGAACGCGATGCGCGAGGCACCGGACGTCATCCTGATCGGCGAGATCCTGGATGCCGAGACGATGGAAGCGG
CCATCGCCTTCGCCGAGACCGGCCATCTGTGCCTGGCCACGCTGCATTCCAACAATGCCGACCAGACCATCGAGCGGATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGACGGCCGCCGTCTTCCAGCCACCGAGGTGCTGATCAACACCCCGATGATCCGCGACCTGCTGC
GACGCGGCCAGGTGCACGAGATCAAGGCAGCGATGGAAGCCTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAATGCCTG
TTCCGGCTCGCCAAGTCCGGCGTGATCGAGCAGGAGGAAGCCTTGCGCGCTGCCGATTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTCTCCGAAGGCAGCAGCGGCGAGCACGACCCGTACGCCGACTATTCAGCAGGCCAGCCGAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

95.733

0.493
  pilU Vibrio cholerae strain A1552

48.098

98.133

0.472
  pilU Acinetobacter baylyi ADP1

49.718

94.4

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.089

95.467

0.44
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.124

90.133

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363