Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RJ646_RS04100 Genome accession   NZ_CP134358
Coordinates   844863..845843 (+) Length   326 a.a.
NCBI ID   WP_310984077.1    Uniprot ID   -
Organism   Escherichia coli strain TUM13936     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 839863..850843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ646_RS04070 (RJ646_04070) yggM 840290..841297 (+) 1008 WP_276270017.1 DUF1202 family protein -
  RJ646_RS04075 (RJ646_04075) hemW 841540..842676 (-) 1137 WP_001625906.1 radical SAM family heme chaperone HemW -
  RJ646_RS04080 (RJ646_04080) rdgB 842669..843262 (-) 594 WP_075843050.1 XTP/dITP diphosphatase -
  RJ646_RS04085 (RJ646_04085) yggU 843270..843560 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  RJ646_RS04090 (RJ646_04090) yggT 843557..844123 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  RJ646_RS04095 (RJ646_04095) yggS 844141..844845 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  RJ646_RS04100 (RJ646_04100) pilT 844863..845843 (+) 981 WP_310984077.1 type IV pilus twitching motility protein PilT Machinery gene
  RJ646_RS04105 (RJ646_04105) ruvX 846035..846451 (-) 417 WP_000017109.1 Holliday junction resolvase RuvX -
  RJ646_RS04110 (RJ646_04110) yqgE 846451..847014 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  RJ646_RS04115 (RJ646_04115) gshB 847123..848073 (-) 951 WP_000593273.1 glutathione synthase -
  RJ646_RS04120 (RJ646_04120) rsmE 848086..848817 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RJ646_RS04125 (RJ646_04125) endA 848897..849604 (-) 708 WP_098031165.1 deoxyribonuclease I -
  RJ646_RS04130 (RJ646_04130) yggI 849699..850196 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35903.04 Da        Isoelectric Point: 5.5860

>NTDB_id=802826 RJ646_RS04100 WP_310984077.1 844863..845843(+) (pilT) [Escherichia coli strain TUM13936]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLTENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGSHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=802826 RJ646_RS04100 WP_310984077.1 844863..845843(+) (pilT) [Escherichia coli strain TUM13936]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGACGGAAAACCAGCGATTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGTGGAAAAT
CTACCACGCTGGCGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTCGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTTGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371