Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P3G59_RS27470 Genome accession   NZ_CP119967
Coordinates   6115932..6116966 (-) Length   344 a.a.
NCBI ID   WP_277759697.1    Uniprot ID   -
Organism   Pseudomonas sp. A34-9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6110932..6121966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3G59_RS27440 ruvX 6111104..6111541 (+) 438 WP_064589634.1 Holliday junction resolvase RuvX -
  P3G59_RS27445 pyrR 6111622..6112128 (+) 507 WP_034153653.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  P3G59_RS27450 - 6112156..6113160 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  P3G59_RS27455 - 6113157..6114428 (+) 1272 WP_277759695.1 dihydroorotase -
  P3G59_RS27460 - 6114512..6114943 (-) 432 WP_277759696.1 TM2 domain-containing protein -
  P3G59_RS27465 - 6115192..6115815 (+) 624 WP_129395899.1 C40 family peptidase -
  P3G59_RS27470 pilT 6115932..6116966 (-) 1035 WP_277759697.1 type IV pilus twitching motility protein PilT Machinery gene
  P3G59_RS27475 - 6117023..6117709 (+) 687 WP_277759698.1 YggS family pyridoxal phosphate-dependent enzyme -
  P3G59_RS27480 proC 6117733..6118551 (+) 819 WP_277759699.1 pyrroline-5-carboxylate reductase -
  P3G59_RS27485 - 6118562..6119152 (+) 591 WP_166221515.1 YggT family protein -
  P3G59_RS27490 - 6119149..6119445 (+) 297 WP_277759700.1 DUF167 domain-containing protein -
  P3G59_RS27495 - 6119673..6120812 (+) 1140 WP_277759701.1 homoserine O-acetyltransferase -
  P3G59_RS27500 metW 6120820..6121440 (+) 621 WP_277759702.1 methionine biosynthesis protein MetW -
  P3G59_RS27505 - 6121467..6121901 (+) 435 WP_277759703.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38069.76 Da        Isoelectric Point: 7.0156

>NTDB_id=801262 P3G59_RS27470 WP_277759697.1 6115932..6116966(-) (pilT) [Pseudomonas sp. A34-9]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDIQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISRDHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=801262 P3G59_RS27470 WP_277759697.1 6115932..6116966(-) (pilT) [Pseudomonas sp. A34-9]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGAGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACATCCAGCGGGTCGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGTGTCGCGCGG
TTCCGGGTTAACGCCTTTAACCAGAACCGGGGTGCCGGAGCGGTGTTCCGGACCATTCCGTCGAAAGTGCTGAGCATGGA
AGATCTAGCCATGGGCGACGTCTTTCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGATTACCTCAACACCCACCGTCATCACCATATCCTGACCATTGAAGAC
CCGATCGAATTCGTTCACGAATCGCGCAAGTGCCTGATCAATCAGCGCGAAGTCCACCGTGATACCCGCAGTTTCGCCAC
CGCGTTGCGTTCAGCGTTGCGCGAAGACCCCGACGTGATCCTGGTCGGCGAGATGCGCGATCTGGAAACCATCCGCCTGG
CGCTGACTGCTGCCGAGACCGGTCACCTGGTGTTCGGCACGCTGCACACCACCTCCGCGGCGAAAACCATCGACCGCGTG
GTCGACGTATTCCCCGGTGACGAGAAGTCGATGGTGCGCTCGATGTTGTCGGAGTCGTTACTGGCGGTGGTCTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGGCGGGTGGCGGCACACGAGATCATGCTGGGGACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACCGGTGGGTCGTTGGGGATGCAGACACTGGACATGTGCCTG
AAGGATCTGGTGACCAAGGGTTTGATCAGCCGCGACCATGCGCGGGAGAAGGCGCGTACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Vibrio cholerae strain A1552

39.71

100

0.398