Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P1P91_RS03010 Genome accession   NZ_CP119391
Coordinates   633646..634680 (+) Length   344 a.a.
NCBI ID   WP_311884424.1    Uniprot ID   -
Organism   Halomonas piscis strain SG2L-4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 628646..639680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P1P91_RS02980 (P1P91_02980) - 629066..629515 (-) 450 WP_311884418.1 DUF4426 domain-containing protein -
  P1P91_RS02985 (P1P91_02985) metW 629573..630169 (-) 597 WP_311885671.1 methionine biosynthesis protein MetW -
  P1P91_RS02990 (P1P91_02990) - 630169..631368 (-) 1200 WP_311884419.1 homoserine O-acetyltransferase -
  P1P91_RS02995 (P1P91_02995) - 631411..632004 (-) 594 WP_311884420.1 YggT family protein -
  P1P91_RS03000 (P1P91_03000) proC 632065..632892 (-) 828 WP_311884421.1 pyrroline-5-carboxylate reductase -
  P1P91_RS03005 (P1P91_03005) - 632923..633615 (-) 693 WP_311884423.1 YggS family pyridoxal phosphate-dependent enzyme -
  P1P91_RS03010 (P1P91_03010) pilT 633646..634680 (+) 1035 WP_311884424.1 type IV pilus twitching motility protein PilT Machinery gene
  P1P91_RS03015 (P1P91_03015) - 634752..635922 (+) 1171 Protein_600 PilT/PilU family type 4a pilus ATPase -
  P1P91_RS03020 (P1P91_03020) - 635934..637058 (-) 1125 WP_311884426.1 anhydro-N-acetylmuramic acid kinase -
  P1P91_RS03025 (P1P91_03025) tyrS 637194..638393 (+) 1200 WP_311884428.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37800.32 Da        Isoelectric Point: 6.2886

>NTDB_id=798768 P1P91_RS03010 WP_311884424.1 633646..634680(+) (pilT) [Halomonas piscis strain SG2L-4]
MDITELLAFAAKQNASDLHLSAGLPPMIRVDGDMRRLDVPEMDNTAVRRLIYDVMNDDQRRDYEEHLEADFSFELPGVAR
FRVNAFNQARGAGAVFRTIPSEVLSMEDLGMGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMVDYVNRHRHEHILTIED
PVEFVHESKRCLVNQREVHRDTRSFADALRSALREGPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRI
IDVFPGAEKGMVRSMLSESLQAVISQTLLKREGGGRVAAHEILVATPAVRNLIREDKIAQIHSAMQTGGSLGMQTLDAAL
IKLVRQGVIGREQAQSQAKAPLAL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=798768 P1P91_RS03010 WP_311884424.1 633646..634680(+) (pilT) [Halomonas piscis strain SG2L-4]
ATGGATATTACTGAACTGCTGGCATTTGCGGCAAAGCAGAACGCCTCTGACCTGCATCTGTCGGCCGGCTTGCCGCCCAT
GATACGCGTTGATGGCGACATGCGAAGGCTGGATGTGCCGGAAATGGATAATACCGCCGTGCGCCGGCTGATCTACGACG
TCATGAATGATGACCAGCGCCGGGACTACGAGGAGCACCTGGAGGCGGACTTCTCGTTCGAGCTGCCGGGCGTCGCGCGC
TTTCGCGTGAACGCTTTCAACCAGGCGCGCGGGGCGGGCGCGGTCTTTCGTACCATCCCAAGCGAAGTGCTGTCCATGGA
AGATCTGGGCATGGGGGAGGTGTTCGAGCGGCTCGCCATGCTGCCGCGGGGGCTGGTGCTGGTCACCGGCCCTACCGGGT
CGGGCAAGAGCACAACGCTTGCGGCCATGGTTGATTACGTCAATCGCCATCGCCACGAGCATATTCTGACCATTGAAGAC
CCGGTGGAATTTGTCCACGAGAGCAAGCGCTGCCTTGTCAACCAGCGCGAAGTGCACCGCGATACCCGCAGCTTTGCCGA
TGCCCTGCGCAGCGCTCTGCGCGAAGGCCCCGACGTGATTCTCGTGGGCGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCATCTGGTGTTTGGCACGCTGCATACGACCTCGGCGGCCAAGACCGTTGACCGTATC
ATCGACGTCTTCCCCGGGGCGGAGAAAGGCATGGTGCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATTTCCCAGAC
GCTGCTCAAGCGCGAGGGCGGCGGGCGCGTGGCCGCCCACGAAATCCTCGTGGCCACGCCGGCGGTGCGCAACCTCATCC
GCGAAGACAAGATTGCCCAGATTCACTCGGCGATGCAGACCGGCGGGAGCCTGGGCATGCAGACGCTTGACGCGGCTTTG
ATCAAGCTGGTGCGACAAGGCGTTATCGGCCGCGAGCAGGCGCAGTCCCAGGCCAAGGCACCGCTGGCGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.419

99.128

0.767
  pilT Pseudomonas aeruginosa PAK

77.419

99.128

0.767
  pilT Acinetobacter nosocomialis M2

73.314

99.128

0.727
  pilT Acinetobacter baumannii D1279779

73.314

99.128

0.727
  pilT Acinetobacter baumannii strain A118

73.314

99.128

0.727
  pilT Acinetobacter baylyi ADP1

72.727

99.128

0.721
  pilT Neisseria meningitidis 8013

69.118

98.837

0.683
  pilT Neisseria gonorrhoeae MS11

68.824

98.837

0.68
  pilT Legionella pneumophila strain Lp02

69.231

98.256

0.68
  pilT Legionella pneumophila strain ERS1305867

69.231

98.256

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.216

99.419

0.529
  pilU Pseudomonas stutzeri DSM 10701

42.687

97.384

0.416
  pilU Acinetobacter baylyi ADP1

41.108

99.709

0.41
  pilU Vibrio cholerae strain A1552

41.791

97.384

0.407