Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PY250_RS12885 Genome accession   NZ_CP119334
Coordinates   2759810..2760850 (-) Length   346 a.a.
NCBI ID   WP_005381015.1    Uniprot ID   A0A2I3C9Z7
Organism   Vibrio alginolyticus strain 2-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2754810..2765850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY250_RS12855 (PY250_12855) endA 2754954..2755649 (+) 696 WP_017635582.1 deoxyribonuclease I -
  PY250_RS12860 (PY250_12860) rsmE 2755763..2756494 (+) 732 WP_205461128.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PY250_RS12865 (PY250_12865) gshB 2756508..2757458 (+) 951 WP_258513869.1 glutathione synthase -
  PY250_RS12870 (PY250_12870) - 2757587..2758150 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  PY250_RS12875 (PY250_12875) ruvX 2758186..2758611 (+) 426 WP_213905016.1 Holliday junction resolvase RuvX -
  PY250_RS12880 (PY250_12880) pilU 2758673..2759779 (-) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PY250_RS12885 (PY250_12885) pilT 2759810..2760850 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  PY250_RS12890 (PY250_12890) - 2760879..2761580 (+) 702 WP_005384521.1 YggS family pyridoxal phosphate-dependent enzyme -
  PY250_RS12895 (PY250_12895) proC 2761740..2762558 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  PY250_RS12900 (PY250_12900) - 2762611..2763168 (+) 558 WP_005381012.1 YggT family protein -
  PY250_RS12905 (PY250_12905) yggU 2763168..2763458 (+) 291 WP_065274862.1 DUF167 family protein YggU -
  PY250_RS12910 (PY250_12910) - 2763587..2764018 (+) 432 WP_213905015.1 DUF4426 domain-containing protein -
  PY250_RS12915 (PY250_12915) - 2764137..2764739 (+) 603 WP_005381009.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38532.02 Da        Isoelectric Point: 6.5039

>NTDB_id=798557 PY250_RS12885 WP_005381015.1 2759810..2760850(-) (pilT) [Vibrio alginolyticus strain 2-1]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=798557 PY250_RS12885 WP_005381015.1 2759810..2760850(-) (pilT) [Vibrio alginolyticus strain 2-1]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGT
TTCCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTATTTCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTAGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCATGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
TGCTTTGCGTAGTGCATTGCGCGAAGACCCAGACGTTATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGTCTAG
CGCTGACAGCGGCTGAAACCGGTCACCTTGTTTTCGGGACGTTGCACACCAGCTCGGCGGCAAAAACCATCGACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTTCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAACGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361