Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FRH_RS07795 Genome accession   NZ_AP022850
Coordinates   1666697..1667740 (+) Length   347 a.a.
NCBI ID   WP_153843140.1    Uniprot ID   -
Organism   Halomonas sp. A020     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1661697..1672740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRH_RS07765 (HaloA020_15410) - 1662046..1662507 (-) 462 WP_139527990.1 DUF4426 domain-containing protein -
  FRH_RS07770 (HaloA020_15420) metW 1662544..1663140 (-) 597 WP_139527991.1 methionine biosynthesis protein MetW -
  FRH_RS07775 (HaloA020_15430) - 1663140..1664336 (-) 1197 WP_139527992.1 homoserine O-acetyltransferase -
  FRH_RS07780 (HaloA020_15440) - 1664411..1665004 (-) 594 WP_219862550.1 YggT family protein -
  FRH_RS07785 (HaloA020_15450) proC 1665065..1665892 (-) 828 WP_219862553.1 pyrroline-5-carboxylate reductase -
  FRH_RS07790 (HaloA020_15460) - 1665965..1666663 (-) 699 WP_153843141.1 YggS family pyridoxal phosphate-dependent enzyme -
  FRH_RS07795 (HaloA020_15470) pilT 1666697..1667740 (+) 1044 WP_153843140.1 type IV pilus twitching motility protein PilT Machinery gene
  FRH_RS07800 (HaloA020_15480) - 1667807..1668949 (-) 1143 WP_219863797.1 anhydro-N-acetylmuramic acid kinase -
  FRH_RS07805 (HaloA020_15490) tyrS 1669066..1670265 (+) 1200 WP_219862555.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38184.69 Da        Isoelectric Point: 6.5071

>NTDB_id=79448 FRH_RS07795 WP_153843140.1 1666697..1667740(+) (pilT) [Halomonas sp. A020]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMENDDVRRLIYDIMNDRQRRDYEEHLEADFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPNKVLSMDALGMGEVFQRLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRFEHILTIED
PVEFVHTSKRCLINQREVHRDTHSFSGALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKSMVRSMLSESLQAVVSQTLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQIYSAIQTGGALGMQTLNASL
AKLVKEGVVSMDDAQARAKGTLTLKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=79448 FRH_RS07795 WP_153843140.1 1666697..1667740(+) (pilT) [Halomonas sp. A020]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAATGCGTCCGACCTGCACCTTTCGGCGGGATTGCCGCCCAT
GATACGTGTTGATGGCGATATTCGTCGGCTTAATGTGCCCGCCATGGAGAACGACGACGTGCGGCGGCTGATCTACGACA
TCATGAACGATCGGCAGCGCCGTGACTACGAAGAGCACCTAGAAGCGGACTTTTCCTTCGAAGTGCCGGGCGTGGCGCGC
TTTAGGGTCAATGCCTTCAACCAGGCACGCGGGGCAGGGGCCGTCTTTCGTACCATTCCCAACAAGGTGCTTTCCATGGA
CGCCTTGGGCATGGGAGAGGTGTTCCAACGCCTGTCGATGCTGCCCAGGGGCTTGGTGCTAGTGACCGGGCCGACGGGGT
CGGGCAAAAGCACGACCCTGGCGGCAATGATCGACTACATCAACGATCACCGCTTCGAGCATATCCTGACCATCGAAGAC
CCGGTGGAGTTTGTGCACACCAGCAAGCGCTGCCTGATCAACCAGCGGGAGGTTCATCGCGATACCCATAGCTTTTCGGG
GGCGTTACGTAGCGCGCTGCGCGAAGATCCGGACGTGATTTTGGTCGGCGAGCTTCGTGATCTCGAAACGATCCGCCTGG
CGCTGACCGCGGCTGAAACGGGACACTTGGTATTTGGGACGCTGCACACCACCTCTGCGGCGAAAACCATTGACAGGATC
ATCGATGTGTTTCCCGGCGATGAGAAATCCATGGTGCGCTCGATGCTCTCCGAATCGCTCCAGGCGGTCGTTTCGCAAAC
GTTGCTGAAACGCCAGGGTGGCGGACGCGTGGCAGCCCACGAGATCCTGATCGCCACGTCGGCGGTTCGTAATCTGATCC
GTGAAGATAAAGTGGCACAGATCTACTCGGCCATTCAGACTGGCGGCGCCCTGGGGATGCAGACACTCAATGCGTCGCTC
GCCAAGTTGGTCAAGGAGGGCGTGGTGAGTATGGACGACGCCCAGGCACGCGCAAAAGGCACGCTGACGTTAAAAGACGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.761

97.695

0.769
  pilT Pseudomonas aeruginosa PAK

78.761

97.695

0.769
  pilT Acinetobacter nosocomialis M2

76.991

97.695

0.752
  pilT Acinetobacter baumannii D1279779

76.991

97.695

0.752
  pilT Acinetobacter baumannii strain A118

76.991

97.695

0.752
  pilT Acinetobacter baylyi ADP1

74.631

97.695

0.729
  pilT Legionella pneumophila strain Lp02

71.302

97.406

0.695
  pilT Legionella pneumophila strain ERS1305867

71.302

97.406

0.695
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.118

0.683
  pilT Vibrio cholerae strain A1552

70.326

97.118

0.683
  pilT Neisseria meningitidis 8013

66.087

99.424

0.657
  pilT Neisseria gonorrhoeae MS11

65.797

99.424

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.392

97.695

0.522
  pilU Acinetobacter baylyi ADP1

40.634

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.751

99.712

0.406
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392

Multiple sequence alignment