Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PWO56_RS03365 Genome accession   NZ_CP118699
Coordinates   676429..677463 (-) Length   344 a.a.
NCBI ID   WP_011707389.1    Uniprot ID   A0A081UZ58
Organism   Aeromonas hydrophila strain K522     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 629078..679269 676429..677463 within 0


Gene organization within MGE regions


Location: 629078..679269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWO56_RS03140 (PWO56_03140) - 629561..629728 (+) 168 WP_270697470.1 hypothetical protein -
  PWO56_RS03145 (PWO56_03145) - 629725..630084 (+) 360 WP_042467308.1 hypothetical protein -
  PWO56_RS03150 (PWO56_03150) - 630081..630335 (+) 255 WP_270697471.1 hypothetical protein -
  PWO56_RS03155 (PWO56_03155) - 630332..633136 (+) 2805 WP_270697472.1 DUF927 domain-containing protein -
  PWO56_RS03160 (PWO56_03160) - 634085..635080 (+) 996 WP_270697473.1 hypothetical protein -
  PWO56_RS03165 (PWO56_03165) - 635326..635553 (+) 228 WP_226031356.1 hypothetical protein -
  PWO56_RS03170 (PWO56_03170) - 635890..636204 (+) 315 WP_270697474.1 hypothetical protein -
  PWO56_RS03175 (PWO56_03175) - 636230..637051 (+) 822 WP_270697475.1 hypothetical protein -
  PWO56_RS03180 (PWO56_03180) - 637142..639604 (+) 2463 WP_270697476.1 phage tail tape measure protein -
  PWO56_RS03185 (PWO56_03185) amt 640791..642227 (+) 1437 WP_077095151.1 ammonium transporter -
  PWO56_RS03190 (PWO56_03190) rlmD 642562..643914 (+) 1353 WP_077095150.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  PWO56_RS03195 (PWO56_03195) - 644120..644302 (+) 183 WP_077095149.1 DUF5363 family protein -
  PWO56_RS03200 (PWO56_03200) tppF 644271..644888 (-) 618 WP_011707416.1 type IVa pilus pseudopilin TppF -
  PWO56_RS03205 (PWO56_03205) - 644929..645717 (+) 789 WP_060390690.1 TatD family hydrolase -
  PWO56_RS03210 (PWO56_03210) - 645931..647205 (+) 1275 WP_011707414.1 NupC/NupG family nucleoside CNT transporter -
  PWO56_RS03215 (PWO56_03215) - 647329..647499 (+) 171 WP_005305103.1 DUF1427 family protein -
  PWO56_RS03220 (PWO56_03220) deoC 647891..648664 (+) 774 WP_077095148.1 deoxyribose-phosphate aldolase -
  PWO56_RS03225 (PWO56_03225) deoA 648774..650105 (+) 1332 WP_016351989.1 thymidine phosphorylase -
  PWO56_RS03230 (PWO56_03230) - 650120..651328 (+) 1209 WP_011707411.1 phosphopentomutase -
  PWO56_RS03235 (PWO56_03235) deoD 651406..652122 (+) 717 WP_011707410.1 purine-nucleoside phosphorylase -
  PWO56_RS03240 (PWO56_03240) - 652192..654438 (-) 2247 WP_190283793.1 PAS domain S-box protein -
  PWO56_RS03245 (PWO56_03245) - 654492..655100 (-) 609 WP_017411238.1 AhpA/YtjB family protein -
  PWO56_RS03250 (PWO56_03250) serB 655221..656231 (+) 1011 WP_077095146.1 phosphoserine phosphatase SerB -
  PWO56_RS03255 (PWO56_03255) - 656281..657186 (+) 906 WP_011707407.1 alpha/beta fold hydrolase -
  PWO56_RS03260 (PWO56_03260) - 657315..659693 (-) 2379 WP_077095145.1 PilZ domain-containing protein -
  PWO56_RS03265 (PWO56_03265) radA 659794..661158 (+) 1365 WP_024945847.1 DNA repair protein RadA -
  PWO56_RS03270 (PWO56_03270) - 661230..661610 (-) 381 WP_011707404.1 PilZ domain-containing protein -
  PWO56_RS03275 (PWO56_03275) - 661712..662194 (-) 483 WP_011707403.1 Rsd/AlgQ family anti-sigma factor -
  PWO56_RS03280 (PWO56_03280) - 662300..663331 (-) 1032 WP_214003924.1 IS630 family transposase -
  PWO56_RS03285 (PWO56_03285) - 664002..664982 (+) 981 WP_026141915.1 IS5-like element IS5 family transposase -
  PWO56_RS03290 (PWO56_03290) - 665130..665345 (-) 216 Protein_620 helix-turn-helix domain-containing protein -
  PWO56_RS03295 (PWO56_03295) - 665466..665651 (+) 186 WP_130631666.1 hypothetical protein -
  PWO56_RS03300 (PWO56_03300) hupA 665710..665982 (+) 273 WP_005305063.1 nucleoid-associated protein HU-alpha -
  PWO56_RS03305 (PWO56_03305) - 666084..666509 (+) 426 WP_010636148.1 hypothetical protein -
  PWO56_RS03310 (PWO56_03310) - 666585..666734 (-) 150 WP_011707400.1 hypothetical protein -
  PWO56_RS03315 (PWO56_03315) dusA 666737..667723 (-) 987 WP_270697458.1 tRNA dihydrouridine(20/20a) synthase DusA -
  PWO56_RS03320 (PWO56_03320) zur 667862..668320 (+) 459 WP_075384504.1 zinc uptake transcriptional repressor Zur -
  PWO56_RS03325 (PWO56_03325) - 668310..668771 (+) 462 WP_016351979.1 chemotaxis protein CheX -
  PWO56_RS03330 (PWO56_03330) - 668776..669006 (+) 231 WP_179101286.1 hypothetical protein -
  PWO56_RS03335 (PWO56_03335) brnQ 669077..670354 (-) 1278 WP_232105266.1 branched-chain amino acid transport system II carrier protein -
  PWO56_RS03340 (PWO56_03340) brnQ 670748..672055 (-) 1308 WP_011707394.1 branched-chain amino acid transport system II carrier protein -
  PWO56_RS03345 (PWO56_03345) - 672247..672957 (-) 711 WP_077095142.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PWO56_RS03350 (PWO56_03350) srmB 673086..674309 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  PWO56_RS03355 (PWO56_03355) yaaA 674486..675259 (-) 774 WP_077095141.1 peroxide stress protein YaaA -
  PWO56_RS03360 (PWO56_03360) pilU 675291..676400 (-) 1110 WP_017411211.1 type IVa pilus ATPase TapU Machinery gene
  PWO56_RS03365 (PWO56_03365) pilT 676429..677463 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  PWO56_RS03370 (PWO56_03370) - 677503..678204 (+) 702 WP_077095140.1 YggS family pyridoxal phosphate-dependent enzyme -
  PWO56_RS03375 (PWO56_03375) proC 678263..679087 (+) 825 WP_077095139.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38287.06 Da        Isoelectric Point: 6.7439

>NTDB_id=794448 PWO56_RS03365 WP_011707389.1 676429..677463(-) (pilT) [Aeromonas hydrophila strain K522]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=794448 PWO56_RS03365 WP_011707389.1 676429..677463(-) (pilT) [Aeromonas hydrophila strain K522]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCACGCCCTCATCTACGACA
TCATGAACGATCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTGGACTTCTCGTTCGAGGTGCCGAACCTGGCTCGC
TTCCGGGTCAACGCCTTCCAGCAGGCACGCGGCTCAGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
AGATTTGGACGCGCCGGAGATCTTTCGCAAGATTGCCGAATACCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGTAAATCCACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTGCACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATTCGCCTCG
CCATGACCGCGGCCGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTCTCAGAGTCGCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCCGCCGTGCGTAACCTCATCC
GGGAAGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCGTAGTGGCGGCGCTGGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081UZ58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.558

97.965

0.75
  pilT Vibrio cholerae strain A1552

76.558

97.965

0.75
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407