Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Herbaro_RS17820 Genome accession   NZ_CP118632
Coordinates   3942356..3943399 (+) Length   347 a.a.
NCBI ID   WP_275010954.1    Uniprot ID   -
Organism   Herbaspirillum sp. WKF16     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3937356..3948399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Herbaro_RS17790 (Herbaro_17790) ubiA 3937938..3938792 (+) 855 WP_275010948.1 4-hydroxybenzoate octaprenyltransferase -
  Herbaro_RS17795 (Herbaro_17795) - 3938841..3939500 (+) 660 WP_275010949.1 thiopurine S-methyltransferase -
  Herbaro_RS17800 (Herbaro_17800) - 3939686..3939913 (-) 228 WP_275010950.1 hypothetical protein -
  Herbaro_RS17805 (Herbaro_17805) - 3939931..3940683 (-) 753 WP_275010951.1 peptidoglycan DD-metalloendopeptidase family protein -
  Herbaro_RS17810 (Herbaro_17810) proC 3940683..3941495 (-) 813 WP_275010952.1 pyrroline-5-carboxylate reductase -
  Herbaro_RS17815 (Herbaro_17815) - 3941580..3942281 (-) 702 WP_275010953.1 YggS family pyridoxal phosphate-dependent enzyme -
  Herbaro_RS17820 (Herbaro_17820) pilT 3942356..3943399 (+) 1044 WP_275010954.1 type IV pilus twitching motility protein PilT Machinery gene
  Herbaro_RS17825 (Herbaro_17825) - 3943490..3943975 (+) 486 WP_275010955.1 glutathione peroxidase -
  Herbaro_RS17830 (Herbaro_17830) - 3943988..3944914 (-) 927 WP_275010956.1 phosphate/phosphite/phosphonate ABC transporter substrate-binding protein -
  Herbaro_RS17835 (Herbaro_17835) - 3944911..3947196 (-) 2286 WP_275014047.1 EAL domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37472.27 Da        Isoelectric Point: 8.5671

>NTDB_id=794119 Herbaro_RS17820 WP_275010954.1 3942356..3943399(+) (pilT) [Herbaspirillum sp. WKF16]
MDIAALLAFAVRNQASDLHLCAGLPPMLRVHGEIRRMDAPPLCDYRLRAMLHGLMDDVQRRRGAERMESDFAFRHEQLGR
FRVNVFRQLRGAAATIRHIPPQPPALAGIGAPAVCADLACRPRGLVLVTGPTGSGKSTTLAAMVHHINERRALHIVTIED
PIEFVHEPLRALVSQREVGTHTGSFADALRAALRQDPDVLLVGELRDAESIRLALTAAETGHLVLATLHTTSAAKTADRI
VDVFPAGERDGARAMLAESLLAVISQTLLPLRDGGGMVAAHEVMLGTPAIRNLIREGRTAQLQTAIQTSAGIGMQTLDAS
LAQLLQRQLISPESARRHGRALDFPSS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=794119 Herbaro_RS17820 WP_275010954.1 3942356..3943399(+) (pilT) [Herbaspirillum sp. WKF16]
ATGGACATTGCCGCGCTGCTGGCATTTGCCGTCAGAAACCAAGCCTCCGACCTGCATCTTTGCGCCGGCCTGCCGCCCAT
GCTGCGCGTGCACGGCGAGATCCGCCGCATGGATGCCCCGCCGCTGTGCGACTACCGGCTGCGCGCCATGCTGCATGGAC
TGATGGACGATGTCCAGCGGCGCCGCGGCGCCGAGCGCATGGAATCCGACTTCGCCTTCCGGCATGAACAGCTGGGACGC
TTTCGCGTCAACGTCTTCCGCCAGCTGCGCGGCGCCGCCGCGACCATCCGCCACATTCCCCCGCAACCGCCGGCGCTGGC
CGGGATCGGCGCGCCGGCCGTCTGCGCCGACCTGGCTTGTCGACCGCGCGGGCTGGTGCTCGTGACCGGGCCCACCGGGT
CGGGCAAGAGCACCACGCTGGCGGCGATGGTGCACCATATCAACGAGCGGCGGGCGCTGCACATCGTGACCATCGAGGAT
CCGATCGAGTTCGTCCACGAGCCGCTGCGCGCGCTGGTCAGCCAGCGCGAGGTGGGCACGCATACCGGCTCCTTCGCCGA
CGCGCTGCGCGCCGCCCTGCGCCAGGATCCGGACGTGCTGCTGGTGGGCGAGCTGCGCGATGCCGAGTCTATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGCTGGCCACGCTGCATACGACCTCGGCGGCCAAGACGGCGGACCGCATC
GTCGACGTCTTCCCCGCCGGCGAAAGGGACGGCGCGCGCGCCATGCTGGCCGAGTCGCTGCTGGCGGTGATCTCGCAGAC
CCTGCTGCCGTTGCGCGACGGCGGCGGCATGGTGGCGGCGCACGAGGTGATGCTGGGCACGCCGGCGATCCGCAACCTGA
TCCGCGAGGGCCGCACGGCGCAGCTCCAGACCGCCATCCAGACCTCGGCCGGGATCGGCATGCAGACGCTGGACGCCAGC
CTGGCCCAGCTGCTGCAGCGCCAACTGATCAGCCCGGAAAGCGCGCGCCGGCATGGGCGCGCGCTGGACTTCCCGAGCTC
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

61.012

96.83

0.591
  pilT Acinetobacter nosocomialis M2

59.821

96.83

0.579
  pilT Acinetobacter baumannii D1279779

59.821

96.83

0.579
  pilT Acinetobacter baumannii strain A118

59.821

96.83

0.579
  pilT Pseudomonas aeruginosa PAK

58.235

97.983

0.571
  pilT Pseudomonas stutzeri DSM 10701

57.647

97.983

0.565
  pilT Neisseria meningitidis 8013

56.38

97.118

0.548
  pilT Legionella pneumophila strain Lp02

55.102

98.847

0.545
  pilT Legionella pneumophila strain ERS1305867

55.102

98.847

0.545
  pilT Neisseria gonorrhoeae MS11

56.083

97.118

0.545
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.655

96.83

0.539
  pilT Vibrio cholerae strain A1552

55.655

96.83

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.146

98.847

0.496
  pilU Vibrio cholerae strain A1552

41.964

96.83

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.42

97.406

0.403
  pilU Acinetobacter baylyi ADP1

39.706

97.983

0.389