Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PVT70_RS03855 Genome accession   NZ_CP118440
Coordinates   855833..856813 (-) Length   326 a.a.
NCBI ID   WP_212807543.1    Uniprot ID   -
Organism   Citrobacter amalonaticus strain DC     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 850833..861813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT70_RS03825 (PVT70_03810) - 851595..852095 (+) 501 WP_044255652.1 SprT family zinc-dependent metalloprotease -
  PVT70_RS03830 (PVT70_03815) endA 852187..852894 (+) 708 WP_061070560.1 deoxyribonuclease I -
  PVT70_RS03835 (PVT70_03820) rsmE 852974..853705 (+) 732 WP_061070561.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PVT70_RS03840 (PVT70_03825) gshB 853718..854665 (+) 948 WP_044255655.1 glutathione synthase -
  PVT70_RS03845 (PVT70_03830) - 854840..855403 (+) 564 WP_042998057.1 YqgE/AlgH family protein -
  PVT70_RS03850 (PVT70_03835) ruvX 855403..855819 (+) 417 WP_042998058.1 Holliday junction resolvase RuvX -
  PVT70_RS03855 (PVT70_03840) pilT 855833..856813 (-) 981 WP_212807543.1 type IV pilus twitching motility protein PilT Machinery gene
  PVT70_RS03860 (PVT70_03845) - 856831..857535 (+) 705 WP_061070564.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVT70_RS03865 (PVT70_03850) - 857554..858120 (+) 567 WP_042998061.1 YggT family protein -
  PVT70_RS03870 (PVT70_03855) yggU 858117..858407 (+) 291 WP_044255664.1 DUF167 family protein YggU -
  PVT70_RS03875 (PVT70_03860) - 858415..859008 (+) 594 WP_044263976.1 XTP/dITP diphosphatase -
  PVT70_RS03880 (PVT70_03865) hemW 859001..860137 (+) 1137 WP_042998064.1 radical SAM family heme chaperone HemW -
  PVT70_RS03885 (PVT70_03870) - 860169..861176 (-) 1008 WP_061070565.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35848.87 Da        Isoelectric Point: 7.0211

>NTDB_id=792568 PVT70_RS03855 WP_212807543.1 855833..856813(-) (pilT) [Citrobacter amalonaticus strain DC]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMERAPFTATDITGLLADWLDEGQQAALRQNGQLDFAVSLADNQRLRA
SAFHQRQGTSLALRLLPTQCPTLETLGTPSVLPELLRSENGLILVTGATGSGKSTTLAAMVTWLNQHIDGHILTLEDPIE
YIYTSQRCLIQQREAGLHCASFATGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDS
FPAQEKDPVRSQLAGSLRAVLSQKLEPDKQEGRVALYEQLINTPAAGNLIREGKSHQLPHVIQTGQQMGMMTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=792568 PVT70_RS03855 WP_212807543.1 855833..856813(-) (pilT) [Citrobacter amalonaticus strain DC]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCGCG
CTGGCGAAAACGGGGACGCATGGAGCGTGCACCCTTTACCGCAACCGACATTACCGGACTGCTGGCGGACTGGCTGGATG
AGGGGCAGCAGGCGGCGTTACGACAAAATGGGCAACTGGATTTCGCGGTTTCGCTCGCGGATAACCAGCGCCTGCGGGCC
AGCGCCTTTCACCAGCGGCAGGGGACGTCGCTGGCGCTACGGCTGCTGCCGACGCAGTGCCCGACGCTGGAAACGCTGGG
GACGCCTTCTGTGCTGCCCGAACTGCTCCGCAGCGAGAATGGTTTGATTCTGGTGACTGGGGCGACAGGCAGCGGAAAAT
CAACTACCCTGGCGGCGATGGTCACGTGGCTGAATCAGCATATCGATGGACATATTCTGACGCTGGAAGATCCCATCGAG
TATATCTACACCAGCCAGCGCTGCCTGATTCAGCAGCGTGAAGCAGGACTGCACTGCGCCTCATTTGCCACCGGATTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGAGAGCTACGCGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGTGCCGCGCAGGCGATCGAGCGACTGATTGATTCT
TTTCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCAGGCAGTCTGCGCGCCGTACTGTCGCAGAAACTGGAACC
CGACAAGCAGGAGGGGCGCGTTGCGCTGTATGAACAGCTGATCAACACGCCGGCAGCGGGGAATTTGATTCGGGAAGGGA
AGTCCCATCAGTTACCCCATGTCATTCAGACCGGGCAGCAGATGGGGATGATGACCTTTGCCCAGAGCCTGCAACAGCGA
CAGGCACAAGGACGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.902

97.239

0.417
  pilU Vibrio cholerae strain A1552

42.581

95.092

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Acinetobacter baylyi ADP1

39.077

99.693

0.39